(Linear) (Six-base) MAPSORT of: KIEE6568.seq  Check: 9259  from: 1  to: 564



KIEE6568.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AatII G_ACGT'C

Cuts at:      0     266     564 
   Size:        266     298
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     564 
   Size:         60     504
 
AfeI AGC'GCT

Cuts at:      0     286     564 
   Size:        286     278
 
AvaI C'yCGr_G

Cuts at:      0      45     564 
   Size:         45     519
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      43      77     564 
   Size:         43      34     487

  Fragments arranged by size: 

                 487      43      34
 
BfrBI ATG'CAT

Cuts at:      0     409     564 
   Size:        409     155
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     564 
   Size:        463     101
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     564 
   Size:        415     149
 
BsaBI GATnn'nnATC

Cuts at:      0     400     564 
   Size:        400     164
 
BsaHI Gr'CG_yC

Cuts at:      0     263     564 
   Size:        263     301
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     197     227     344     374     564 
   Size:        197      30     117      30     190

  Fragments arranged by size: 

                 197     190     117      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     365     564 
   Size:        365     199
 
BsiEI CG_ry'CG

Cuts at:      0      57      70     564 
   Size:         57      13     494

  Fragments arranged by size: 

                 494      57      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     125     564 
   Size:        125     439
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     141     564 
   Size:        141     423
 
EaeI y'GGCC_r

Cuts at:      0      67     564 
   Size:         67     497
 
EagI C'GGCC_G

Cuts at:      0      67     564 
   Size:         67     497
 
EarI CTCTTCn'nnn_

Cuts at:      0     466     564 
   Size:        466      98
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     463     564 
   Size:         60     403     101

  Fragments arranged by size: 

                 403     101      60
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     283     564 
   Size:        283     281
 
HaeII r_GCGC'y

Cuts at:      0     288     564 
   Size:        288     276
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     344     376     564 
   Size:        344      32     188

  Fragments arranged by size: 

                 344     188      32
 
NsiI A_TGCA'T

Cuts at:      0     411     564 
   Size:        411     153
 
PfoI T'CCnGG_A

Cuts at:      0     494     564 
   Size:        494      70
 
PvuI CG_AT'CG

Cuts at:      0      57     564 
   Size:         57     507
 
SexAI A'CCwGG_T

Cuts at:      0     140     564 
   Size:        140     424
 
SmaI CCC'GGG

Cuts at:      0      47     564 
   Size:         47     517
 
SmlI C'TyrA_G

Cuts at:      0     430     564 
   Size:        430     134
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     197     200     229     232     564 
   Size:        197       3      29       3     332

  Fragments arranged by size: 

                 332     197      29       3       3
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      26     141     564 
   Size:         26     115     423

  Fragments arranged by size: 

                 423     115      26
 
XmaI C'CCGG_G

Cuts at:      0      45     564 
   Size:         45     519
 
ZraI GAC'GTC

Cuts at:      0     264     564 
   Size:        264     300
 

 Enzymes that do cut:

    AatII     AcuI     AfeI     AvaI     BcgI    BfrBI     BpmI    BpuEI
    BsaBI    BsaHI    BsaXI    BseRI    BsiEI    BspMI    BstXI     EaeI
     EagI     EarI  Eco57MI     FalI    HaeII    Hin4I     NsiI     PfoI
     PvuI    SexAI     SmaI     SmlI     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaWI
    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
  Tth111I     XbaI     XhoI     XmnI