(Linear) (Six-base) MAPSORT of: KIEE6567.seq  Check: 5895  from: 1  to: 543



KIEE6567.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AvaI C'yCGr_G

Cuts at:      0      56     166     543 
   Size:         56     110     377

  Fragments arranged by size: 

                 377     110      56
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     456     543 
   Size:        456      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330     543 
   Size:        330     213
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25     498     528     543 
   Size:         25     473      30      15

  Fragments arranged by size: 

                 473      30      25      15
 
BseYI C'CCAG_C

Cuts at:      0     224     468     543 
   Size:        224     244      75

  Fragments arranged by size: 

                 244     224      75
 
BsiEI CG_ry'CG

Cuts at:      0     316     543 
   Size:        316     227
 
BtgI C'CryG_G

Cuts at:      0     310     449     543 
   Size:        310     139      94

  Fragments arranged by size: 

                 310     139      94
 
BtsI GCAGTG_nn'

Cuts at:      0     463     543 
   Size:        463      80
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      23     543 
   Size:         23     520
 
EaeI y'GGCC_r

Cuts at:      0     313     543 
   Size:        313     230
 
EagI C'GGCC_G

Cuts at:      0     313     543 
   Size:        313     230
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330     543 
   Size:        330     213
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     323     543 
   Size:        323     220
 
EcoO109I rG'GnC_Cy

Cuts at:      0     521     543 
   Size:        521      22
 
EcoRV GAT'ATC

Cuts at:      0     129     543 
   Size:        129     414
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     543 
   Size:        386     157
 
MspA1I CmG'CkG

Cuts at:      0     182     543 
   Size:        182     361
 
PpuMI rG'GwC_Cy

Cuts at:      0     521     543 
   Size:        521      22
 
PstI C_TGCA'G

Cuts at:      0     324     543 
   Size:        324     219
 
SbfI CC_TGCA'GG

Cuts at:      0     324     543 
   Size:        324     219
 
SfcI C'TryA_G

Cuts at:      0     320     543 
   Size:        320     223
 
StyI C'CwwG_G

Cuts at:      0     199     543 
   Size:        199     344
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27     543 
   Size:         27     516
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     394     447     543 
   Size:        394      53      96

  Fragments arranged by size: 

                 394      96      53
 

 Enzymes that do cut:

     AvaI    BciVI     BpmI    BsaXI    BseYI    BsiEI     BtgI     BtsI
     DrdI     EaeI     EagI  Eco57MI    EcoNI EcoO109I    EcoRV     MmeI
   MspA1I    PpuMI     PstI     SbfI     SfcI     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     EarI     EciI  EcoICRI    EcoRI     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI