(Linear) (Six-base) MAPSORT of: KIEE6566.seq  Check: 5018  from: 1  to: 552



KIEE6566.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AccI GT'mk_AC

Cuts at:      0     404     552 
   Size:        404     148
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     552 
   Size:        341     211
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     377     409     552 
   Size:        377      32     143

  Fragments arranged by size: 

                 377     143      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      56     552 
   Size:         56     496
 
ApoI r'AATT_y

Cuts at:      0     442     518     552 
   Size:        442      76      34

  Fragments arranged by size: 

                 442      76      34
 
AvaI C'yCGr_G

Cuts at:      0     365     552 
   Size:        365     187
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      60      93     552 
   Size:         60      33     459

  Fragments arranged by size: 

                 459      60      33
 
BamHI G'GATC_C

Cuts at:      0     120     552 
   Size:        120     432
 
Bme1580I G_kGCm'C

Cuts at:      0     319     552 
   Size:        319     233
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      68     442     552 
   Size:         68     374     110

  Fragments arranged by size: 

                 374     110      68
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     552 
   Size:        264     288
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     552 
   Size:        316     236
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     325     552 
   Size:        325     227
 
Bsp1286I G_dGCh'C

Cuts at:      0     319     552 
   Size:        319     233
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     105     552 
   Size:        105     447
 
BstYI r'GATC_y

Cuts at:      0     120     495     552 
   Size:        120     375      57

  Fragments arranged by size: 

                 375     120      57
 
BstZ17I GTA'TAC

Cuts at:      0     405     552 
   Size:        405     147
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     341     552 
   Size:        264      77     211

  Fragments arranged by size: 

                 264     211      77
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     310     552 
   Size:        310     242
 
HaeII r_GCGC'y

Cuts at:      0     277     552 
   Size:        277     275
 
MfeI C'AATT_G

Cuts at:      0     309     552 
   Size:        309     243
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     552 
   Size:         86     466
 
PspXI vC'TCGA_Gb

Cuts at:      0     365     552 
   Size:        365     187
 
SmlI C'TyrA_G

Cuts at:      0     295     365     552 
   Size:        295      70     187

  Fragments arranged by size: 

                 295     187      70
 
StuI AGG'CCT

Cuts at:      0     479     552 
   Size:        479      73
 
StyI C'CwwG_G

Cuts at:      0      80     552 
   Size:         80     472
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     364     552 
   Size:        364     188
 
XhoI C'TCGA_G

Cuts at:      0     365     552 
   Size:        365     187
 

 Enzymes that do cut:

     AccI     AcuI     AloI    AlwNI     ApoI     AvaI     BaeI    BamHI
 Bme1580I     BmrI     BpmI    BpuEI     BsaI Bsp1286I   BstAPI    BstYI
  BstZ17I  Eco57MI     FalI    HaeII     MfeI     MmeI    PspXI     SmlI
     StuI     StyI    TaqII     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI