(Linear) (Six-base) MAPSORT of: KIEE6558.seq  Check: 7138  from: 1  to: 468



KIEE6558.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     445     468 
   Size:        445      23
 
ApaLI G'TGCA_C

Cuts at:      0     349     468 
   Size:        349     119
 
AvaI C'yCGr_G

Cuts at:      0      44     468 
   Size:         44     424
 
BanII G_rGCy'C

Cuts at:      0     130     468 
   Size:        130     338
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     281     468 
   Size:        281     187
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     358     392     468 
   Size:        358      34      76

  Fragments arranged by size: 

                 358      76      34
 
Bme1580I G_kGCm'C

Cuts at:      0     353     468 
   Size:        353     115
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     138     468 
   Size:        138     330
 
BseYI C'CCAG_C

Cuts at:      0      83     321     468 
   Size:         83     238     147

  Fragments arranged by size: 

                 238     147      83
 
BsiHKAI G_wGCw'C

Cuts at:      0     353     468 
   Size:        353     115
 
Bsp1286I G_dGCh'C

Cuts at:      0     130     353     468 
   Size:        130     223     115

  Fragments arranged by size: 

                 223     130     115
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     419     468 
   Size:        419      49
 
BsrDI GCAATG_nn'

Cuts at:      0     379     468 
   Size:        379      89
 
BsrFI r'CCGG_y

Cuts at:      0       3      93     468 
   Size:          3      90     375

  Fragments arranged by size: 

                 375      90       3
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     420     468 
   Size:        420      48
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     192     468 
   Size:        192     276
 
BtgI C'CryG_G

Cuts at:      0      59     191     468 
   Size:         59     132     277

  Fragments arranged by size: 

                 277     132      59
 
BtsI GCAGTG_nn'

Cuts at:      0     421     468 
   Size:        421      47
 
EaeI y'GGCC_r

Cuts at:      0     365     468 
   Size:        365     103
 
HaeII r_GCGC'y

Cuts at:      0      10     207     468 
   Size:         10     197     261

  Fragments arranged by size: 

                 261     197      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      94     126     468 
   Size:         94      32     342

  Fragments arranged by size: 

                 342      94      32
 
HincII GTy'rAC

Cuts at:      0     222     468 
   Size:        222     246
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     468 
   Size:        103     365
 
NaeI GCC'GGC

Cuts at:      0       5      95     468 
   Size:          5      90     373

  Fragments arranged by size: 

                 373      90       5
 
NcoI C'CATG_G

Cuts at:      0      59     468 
   Size:         59     409
 
NgoMIV G'CCGG_C

Cuts at:      0       3      93     468 
   Size:          3      90     375

  Fragments arranged by size: 

                 375      90       3
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     391     423     468 
   Size:        391      32      45

  Fragments arranged by size: 

                 391      45      32
 
PstI C_TGCA'G

Cuts at:      0     141     416     468 
   Size:        141     275      52

  Fragments arranged by size: 

                 275     141      52
 
SfcI C'TryA_G

Cuts at:      0     137     412     468 
   Size:        137     275      56

  Fragments arranged by size: 

                 275     137      56
 
StyI C'CwwG_G

Cuts at:      0      59     173     314     468 
   Size:         59     114     141     154

  Fragments arranged by size: 

                 154     141     114      59
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     120     408     468 
   Size:        120     288      60

  Fragments arranged by size: 

                 288     120      60
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     192     468 
   Size:        192     276
 

 Enzymes that do cut:

    AlwNI    ApaLI     AvaI    BanII     BbsI     BcgI Bme1580I     BsaI
    BseYI  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI   BstAPI    BstXI
     BtgI     BtsI     EaeI    HaeII    Hin4I   HincII     MmeI     NaeI
     NcoI   NgoMIV     PsrI     PstI     SfcI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NarI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI