Restriction map for
pFN21AE6554
(Linear) (Six-base) MAPSORT of: KIEE6554.seq Check: 3912 from: 1 to: 456
KIEE6554.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 456
Size: 211 245
AflIII A'CryG_T
Cuts at: 0 179 456
Size: 179 277
ApaI G_GGCC'C
Cuts at: 0 104 214 456
Size: 104 110 242
Fragments arranged by size:
242 110 104
AvaI C'yCGr_G
Cuts at: 0 6 456
Size: 6 450
BanI G'GyrC_C
Cuts at: 0 444 456
Size: 444 12
BanII G_rGCy'C
Cuts at: 0 29 104 214 456
Size: 29 75 110 242
Fragments arranged by size:
242 110 75 29
BglI GCCn_nnn'nGGC
Cuts at: 0 76 456
Size: 76 380
Bme1580I G_kGCm'C
Cuts at: 0 52 104 214 236 456
Size: 52 52 110 22 220
Fragments arranged by size:
220 110 52 52 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 456
Size: 111 345
BsaHI Gr'CG_yC
Cuts at: 0 68 456
Size: 68 388
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 456
Size: 31 425
BseYI C'CCAG_C
Cuts at: 0 83 185 214 449 456
Size: 83 102 29 235 7
Fragments arranged by size:
235 102 83 29 7
BsiHKAI G_wGCw'C
Cuts at: 0 29 456
Size: 29 427
Bsp1286I G_dGCh'C
Cuts at: 0 29 52 104 214 236 456
Size: 29 23 52 110 22 220
Fragments arranged by size:
220 110 52 29 23 22
BsrFI r'CCGG_y
Cuts at: 0 75 456
Size: 75 381
BstXI CCAn_nnnn'nTGG
Cuts at: 0 372 456
Size: 372 84
BtgI C'CryG_G
Cuts at: 0 365 456
Size: 365 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 144 456
Size: 144 312
EaeI y'GGCC_r
Cuts at: 0 173 326 362 456
Size: 173 153 36 94
Fragments arranged by size:
173 153 94 36
EarI CTCTTCn'nnn_
Cuts at: 0 127 456
Size: 127 329
EcoICRI GAG'CTC
Cuts at: 0 27 456
Size: 27 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 211 456
Size: 111 100 245
Fragments arranged by size:
245 111 100
EcoO109I rG'GnC_Cy
Cuts at: 0 210 211 456
Size: 210 1 245
Fragments arranged by size:
245 210 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 456
Size: 363 93
HincII GTy'rAC
Cuts at: 0 396 456
Size: 396 60
MluI A'CGCG_T
Cuts at: 0 179 456
Size: 179 277
MscI TGG'CCA
Cuts at: 0 175 364 456
Size: 175 189 92
Fragments arranged by size:
189 175 92
MslI CAynn'nnrTG
Cuts at: 0 109 385 456
Size: 109 276 71
Fragments arranged by size:
276 109 71
MspA1I CmG'CkG
Cuts at: 0 83 189 456
Size: 83 106 267
Fragments arranged by size:
267 106 83
NaeI GCC'GGC
Cuts at: 0 77 456
Size: 77 379
NgoMIV G'CCGG_C
Cuts at: 0 75 456
Size: 75 381
NspI r_CATG'y
Cuts at: 0 153 456
Size: 153 303
PspOMI G'GGCC_C
Cuts at: 0 100 210 456
Size: 100 110 246
Fragments arranged by size:
246 110 100
PstI C_TGCA'G
Cuts at: 0 272 456
Size: 272 184
PvuII CAG'CTG
Cuts at: 0 189 456
Size: 189 267
SacI G_AGCT'C
Cuts at: 0 29 456
Size: 29 427
SapI GCTCTTCn'nnn_
Cuts at: 0 127 456
Size: 127 329
SfcI C'TryA_G
Cuts at: 0 268 456
Size: 268 188
SphI G_CATG'C
Cuts at: 0 153 456
Size: 153 303
Enzymes that do cut:
AcuI AflIII ApaI AvaI BanI BanII BglI Bme1580I
BpmI BsaHI BseRI BseYI BsiHKAI Bsp1286I BsrFI BstXI
BtgI BtgZI EaeI EarI EcoICRI Eco57MI EcoO109I FalI
HincII MluI MscI MslI MspA1I NaeI NgoMIV NspI
PspOMI PstI PvuII SacI SapI SfcI SphI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MmeI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI