(Linear) (Six-base) MAPSORT of: KIEE6553.seq  Check: 2198  from: 1  to: 441



KIEE6553.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     441 
   Size:         67     374
 
AseI AT'TA_AT

Cuts at:      0     265     441 
   Size:        265     176
 
BglII A'GATC_T

Cuts at:      0     340     441 
   Size:        340     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     441 
   Size:        241     200
 
BsaBI GATnn'nnATC

Cuts at:      0     122     441 
   Size:        122     319
 
BseYI C'CCAG_C

Cuts at:      0      53     330     411     441 
   Size:         53     277      81      30

  Fragments arranged by size: 

                 277      81      53      30
 
BsmI GAATG_Cn'

Cuts at:      0     221     441 
   Size:        221     220
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     289     441 
   Size:        289     152
 
BstYI r'GATC_y

Cuts at:      0     123     340     441 
   Size:        123     217     101

  Fragments arranged by size: 

                 217     123     101
 
BtsI GCAGTG_nn'

Cuts at:      0     120     441 
   Size:        120     321
 
EarI CTCTTCn'nnn_

Cuts at:      0      56     441 
   Size:         56     385
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     241     441 
   Size:         67     174     200

  Fragments arranged by size: 

                 200     174      67
 
EcoO109I rG'GnC_Cy

Cuts at:      0     406     441 
   Size:        406      35
 
EcoRV GAT'ATC

Cuts at:      0     198     441 
   Size:        198     243
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     115     147     187     219     332     364     441 
   Size:        115      32      40      32     113      32      77

  Fragments arranged by size: 

                 115     113      77      40      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0      32     441 
   Size:         32     409
 

 Enzymes that do cut:

     AcuI     AseI    BglII     BpmI    BsaBI    BseYI     BsmI    BsmBI
    BstYI     BtsI     EarI  Eco57MI EcoO109I    EcoRV    Hin4I  HindIII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI