(Linear) (Six-base) MAPSORT of: KIEE6551.seq  Check: 687  from: 1  to: 396



KIEE6551.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      77     396 
   Size:         77     319
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     333     396 
   Size:        333      63
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     157     189     396 
   Size:        157      32     207

  Fragments arranged by size: 

                 207     157      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     365     396 
   Size:        365      31
 
BanI G'GyrC_C

Cuts at:      0     309     396 
   Size:        309      87
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      30     396 
   Size:         30     366
 
BglII A'GATC_T

Cuts at:      0     211     396 
   Size:        211     185
 
BlpI GC'TnA_GC

Cuts at:      0     382     396 
   Size:        382      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     108     396 
   Size:         94      14     288

  Fragments arranged by size: 

                 288      94      14
 
BsiHKAI G_wGCw'C

Cuts at:      0     383     396 
   Size:        383      13
 
Bsp1286I G_dGCh'C

Cuts at:      0     383     396 
   Size:        383      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      77     396 
   Size:         77     319
 
BsrFI r'CCGG_y

Cuts at:      0     306     396 
   Size:        306      90
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     296     365     396 
   Size:        296      69      31

  Fragments arranged by size: 

                 296      69      31
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      33     396 
   Size:         33     363
 
BstYI r'GATC_y

Cuts at:      0     211     396 
   Size:        211     185
 
Bsu36I CC'TnA_GG

Cuts at:      0      77     396 
   Size:         77     319
 
BtsI GCAGTG_nn'

Cuts at:      0      53     396 
   Size:         53     343
 
EaeI y'GGCC_r

Cuts at:      0      23     281     396 
   Size:         23     258     115

  Fragments arranged by size: 

                 258     115      23
 
HaeII r_GCGC'y

Cuts at:      0     163     365     396 
   Size:        163     202      31

  Fragments arranged by size: 

                 202     163      31
 
MscI TGG'CCA

Cuts at:      0      25     283     396 
   Size:         25     258     113

  Fragments arranged by size: 

                 258     113      25
 
MspA1I CmG'CkG

Cuts at:      0     296     396 
   Size:        296     100
 
NaeI GCC'GGC

Cuts at:      0     308     396 
   Size:        308      88
 
NgoMIV G'CCGG_C

Cuts at:      0     306     396 
   Size:        306      90
 
PvuII CAG'CTG

Cuts at:      0     296     396 
   Size:        296     100
 

 Enzymes that do cut:

     AarI     AlfI     AloI    AlwNI     BanI     BbsI    BglII     BlpI
    BseRI  BsiHKAI Bsp1286I    BspMI    BsrFI   BstAPI    BstXI    BstYI
   Bsu36I     BtsI     EaeI    HaeII     MscI   MspA1I     NaeI   NgoMIV
    PvuII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI