(Linear) (Six-base) MAPSORT of: KIEE6550.seq  Check: 7183  from: 1  to: 366



KIEE6550.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AleI CACnn'nnGTG

Cuts at:      0     142     366 
   Size:        142     224
 
AvaI C'yCGr_G

Cuts at:      0     332     366 
   Size:        332      34
 
BanII G_rGCy'C

Cuts at:      0     136     366 
   Size:        136     230
 
Bme1580I G_kGCm'C

Cuts at:      0     256     366 
   Size:        256     110
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     366 
   Size:        193     173
 
BsaHI Gr'CG_yC

Cuts at:      0     296     366 
   Size:        296      70
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     366 
   Size:         43     323
 
Bsp1286I G_dGCh'C

Cuts at:      0     136     256     366 
   Size:        136     120     110

  Fragments arranged by size: 

                 136     120     110
 
BspHI T'CATG_A

Cuts at:      0     282     366 
   Size:        282      84
 
BsrDI GCAATG_nn'

Cuts at:      0      64     366 
   Size:         64     302
 
BsrFI r'CCGG_y

Cuts at:      0     235     366 
   Size:        235     131
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     144     366 
   Size:        144     222
 
Bsu36I CC'TnA_GG

Cuts at:      0      55     366 
   Size:         55     311
 
BtgI C'CryG_G

Cuts at:      0       8     155     366 
   Size:          8     147     211

  Fragments arranged by size: 

                 211     147       8
 
BtsI GCAGTG_nn'

Cuts at:      0      89     112     208     366 
   Size:         89      23      96     158

  Fragments arranged by size: 

                 158      96      89      23
 
EaeI y'GGCC_r

Cuts at:      0     205     272     366 
   Size:        205      67      94

  Fragments arranged by size: 

                 205      94      67
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     222     366 
   Size:        222     144
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     366 
   Size:        193     173
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     110     142     366 
   Size:        110      32     224

  Fragments arranged by size: 

                 224     110      32
 
MscI TGG'CCA

Cuts at:      0     207     274     366 
   Size:        207      67      92

  Fragments arranged by size: 

                 207      92      67
 
MslI CAynn'nnrTG

Cuts at:      0     142     366 
   Size:        142     224
 
NaeI GCC'GGC

Cuts at:      0     237     366 
   Size:        237     129
 
NgoMIV G'CCGG_C

Cuts at:      0     235     366 
   Size:        235     131
 
PasI CC'CwG_GG

Cuts at:      0     249     366 
   Size:        249     117
 
PstI C_TGCA'G

Cuts at:      0     314     366 
   Size:        314      52
 
SfcI C'TryA_G

Cuts at:      0     310     366 
   Size:        310      56
 
StyI C'CwwG_G

Cuts at:      0     164     366 
   Size:        164     202
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     132     366 
   Size:        132     234
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     187     219     366 
   Size:        187      32     147

  Fragments arranged by size: 

                 187     147      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     162     366 
   Size:        162     204
 

 Enzymes that do cut:

     AleI     AvaI    BanII Bme1580I     BpmI    BsaHI    BseRI Bsp1286I
    BspHI    BsrDI    BsrFI    BstXI   Bsu36I     BtgI     BtsI     EaeI
     EciI  Eco57MI    Hin4I     MscI     MslI     NaeI   NgoMIV     PasI
     PstI     SfcI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI