(Linear) (Six-base) MAPSORT of: KIEE6547.seq  Check: 2395  from: 1  to: 303



KIEE6547.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AleI CACnn'nnGTG

Cuts at:      0     115     303 
   Size:        115     188
 
AlwNI CAG_nnn'CTG

Cuts at:      0     140     171     303 
   Size:        140      31     132

  Fragments arranged by size: 

                 140     132      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     187     303 
   Size:        187     116
 
Bme1580I G_kGCm'C

Cuts at:      0       7     303 
   Size:          7     296
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     150     303 
   Size:        150     153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     303 
   Size:        117     186
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      29     303 
   Size:         29     274
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       4      97     303 
   Size:          4      93     206

  Fragments arranged by size: 

                 206      93       4
 
BseYI C'CCAG_C

Cuts at:      0      18      77     303 
   Size:         18      59     226

  Fragments arranged by size: 

                 226      59      18
 
BsiHKAI G_wGCw'C

Cuts at:      0     250     303 
   Size:        250      53
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     250     303 
   Size:          7     243      53

  Fragments arranged by size: 

                 243      53       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     197     303 
   Size:        197     106
 
BsrGI T'GTAC_A

Cuts at:      0     221     303 
   Size:        221      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     303 
   Size:        117     186
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     112     303 
   Size:        112     191
 
EcoO109I rG'GnC_Cy

Cuts at:      0      65     303 
   Size:         65     238
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      41      73     303 
   Size:         41      32     230

  Fragments arranged by size: 

                 230      41      32
 
MslI CAynn'nnrTG

Cuts at:      0     115     303 
   Size:        115     188
 
MspA1I CmG'CkG

Cuts at:      0     196     303 
   Size:        196     107
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     140     303 
   Size:        140     163
 
PpuMI rG'GwC_Cy

Cuts at:      0      65     303 
   Size:         65     238
 
SanDI GG'GwC_CC

Cuts at:      0      65     303 
   Size:         65     238
 
SfcI C'TryA_G

Cuts at:      0     197     303 
   Size:        197     106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     127     303 
   Size:        127     176
 
TatI w'GTAC_w

Cuts at:      0     221     303 
   Size:        221      82
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      31     303 
   Size:         31     272
 

 Enzymes that do cut:

     AleI    AlwNI     BbsI Bme1580I     BmrI     BpmI    BsaXI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspMI    BsrGI  Eco57MI    EcoNI EcoO109I
    Hin4I     MslI   MspA1I    PflMI    PpuMI    SanDI     SfcI    TaqII
     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI