(Linear) (Six-base) MAPSORT of: KIEE6542.seq  Check: 3576  from: 1  to: 294



KIEE6542.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

BanII G_rGCy'C

Cuts at:      0     220     294 
   Size:        220      74
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     294 
   Size:         85     209
 
BsaBI GATnn'nnATC

Cuts at:      0     161     294 
   Size:        161     133
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      44      74     294 
   Size:         44      30     220

  Fragments arranged by size: 

                 220      44      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82     294 
   Size:         82     212
 
BsiHKAI G_wGCw'C

Cuts at:      0     220     294 
   Size:        220      74
 
Bsp1286I G_dGCh'C

Cuts at:      0     220     294 
   Size:        220      74
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      99     294 
   Size:         99     195
 
EcoICRI GAG'CTC

Cuts at:      0     218     294 
   Size:        218      76
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     294 
   Size:         85     209
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     165     294 
   Size:        165     129
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     294 
   Size:        116     178
 
PfoI T'CCnGG_A

Cuts at:      0      62     152     220     294 
   Size:         62      90      68      74

  Fragments arranged by size: 

                  90      74      68      62
 
SacI G_AGCT'C

Cuts at:      0     220     294 
   Size:        220      74
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      99     294 
   Size:         99     195
 

 Enzymes that do cut:

    BanII     BpmI    BsaBI    BsaXI    BseRI  BsiHKAI Bsp1286I    BstXI
  EcoICRI  Eco57MI     FalI     MmeI     PfoI     SacI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI