(Linear) (Six-base) MAPSORT of: KIEE6539.seq  Check: 32  from: 1  to: 273



KIEE6539.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

ApoI r'AATT_y

Cuts at:      0     137     273 
   Size:        137     136
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      34     273 
   Size:         34     239
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     121     240     273 
   Size:        121     119      33

  Fragments arranged by size: 

                 121     119      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     273 
   Size:        103     170
 
Bpu10I CC'TnA_GC

Cuts at:      0     158     273 
   Size:        158     115
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     108     273 
   Size:        108     165
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      78     273 
   Size:         78     195
 
BtsI GCAGTG_nn'

Cuts at:      0      96     108     273 
   Size:         96      12     165

  Fragments arranged by size: 

                 165      96      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     273 
   Size:        103     170
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      24      56     273 
   Size:         24      32     217

  Fragments arranged by size: 

                 217      32      24
 
PstI C_TGCA'G

Cuts at:      0     115     273 
   Size:        115     158
 
SfcI C'TryA_G

Cuts at:      0     111     273 
   Size:        111     162
 
StyI C'CwwG_G

Cuts at:      0     183     273 
   Size:        183      90
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     246     273 
   Size:        246      27
 
XmnI GAAnn'nnTTC

Cuts at:      0     217     273 
   Size:        217      56
 

 Enzymes that do cut:

     ApoI     BbsI     BmrI     BpmI   Bpu10I     BsgI   BstAPI     BtsI
  Eco57MI    Hin4I     PstI     SfcI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI