Restriction map for
pFN21AE6537
(Linear) (Six-base) MAPSORT of: KIEE6537.seq Check: 3001 from: 1 to: 234
KIEE6537.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 10 42 234
Size: 10 32 192
Fragments arranged by size:
192 32 10
ApaI G_GGCC'C
Cuts at: 0 166 172 234
Size: 166 6 62
Fragments arranged by size:
166 62 6
AvaI C'yCGr_G
Cuts at: 0 165 200 234
Size: 165 35 34
Fragments arranged by size:
165 35 34
BanI G'GyrC_C
Cuts at: 0 73 234
Size: 73 161
BanII G_rGCy'C
Cuts at: 0 166 172 234
Size: 166 6 62
Fragments arranged by size:
166 62 6
BbeI G_GCGC'C
Cuts at: 0 77 234
Size: 77 157
BglI GCCn_nnn'nGGC
Cuts at: 0 82 161 234
Size: 82 79 73
Fragments arranged by size:
82 79 73
Bme1580I G_kGCm'C
Cuts at: 0 166 172 234
Size: 166 6 62
Fragments arranged by size:
166 62 6
BsaHI Gr'CG_yC
Cuts at: 0 74 234
Size: 74 160
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 179 209 234
Size: 10 30 139 30 25
Fragments arranged by size:
139 30 30 25 10
BseYI C'CCAG_C
Cuts at: 0 190 223 234
Size: 190 33 11
Fragments arranged by size:
190 33 11
BsiEI CG_ry'CG
Cuts at: 0 86 156 234
Size: 86 70 78
Fragments arranged by size:
86 78 70
Bsp1286I G_dGCh'C
Cuts at: 0 166 172 234
Size: 166 6 62
Fragments arranged by size:
166 62 6
BsrBI CCG'CTC
Cuts at: 0 143 234
Size: 143 91
EaeI y'GGCC_r
Cuts at: 0 83 153 234
Size: 83 70 81
Fragments arranged by size:
83 81 70
EagI C'GGCC_G
Cuts at: 0 83 153 234
Size: 83 70 81
Fragments arranged by size:
83 81 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 200 234
Size: 200 34
EcoO109I rG'GnC_Cy
Cuts at: 0 162 169 178 234
Size: 162 7 9 56
Fragments arranged by size:
162 56 9 7
HaeII r_GCGC'y
Cuts at: 0 77 234
Size: 77 157
KasI G'GCGC_C
Cuts at: 0 73 234
Size: 73 161
MspA1I CmG'CkG
Cuts at: 0 60 227 234
Size: 60 167 7
Fragments arranged by size:
167 60 7
NarI GG'CG_CC
Cuts at: 0 74 234
Size: 74 160
NotI GC'GGCC_GC
Cuts at: 0 83 234
Size: 83 151
PasI CC'CwG_GG
Cuts at: 0 172 234
Size: 172 62
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 234
Size: 10 32 192
Fragments arranged by size:
192 32 10
PspOMI G'GGCC_C
Cuts at: 0 162 168 234
Size: 162 6 66
Fragments arranged by size:
162 66 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 161 234
Size: 161 73
SfoI GGC'GCC
Cuts at: 0 75 234
Size: 75 159
SmaI CCC'GGG
Cuts at: 0 167 202 234
Size: 167 35 32
Fragments arranged by size:
167 35 32
SrfI GCCC'GGGC
Cuts at: 0 167 234
Size: 167 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 234
Size: 179 32 23
Fragments arranged by size:
179 32 23
XbaI T'CTAG_A
Cuts at: 0 48 234
Size: 48 186
XmaI C'CCGG_G
Cuts at: 0 165 200 234
Size: 165 35 34
Fragments arranged by size:
165 35 34
Enzymes that do cut:
AloI ApaI AvaI BanI BanII BbeI BglI Bme1580I
BsaHI BsaXI BseYI BsiEI Bsp1286I BsrBI EaeI EagI
EciI EcoO109I HaeII KasI MspA1I NarI NotI PasI
PpiI PspOMI SfiI SfoI SmaI SrfI TstI XbaI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NaeI NcoI NdeI NgoMIV NheI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI Tth111I XcmI
XhoI XmnI ZraI