(Linear) (Six-base) MAPSORT of: KIEE6536.seq  Check: 5909  from: 1  to: 213



KIEE6536.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AflIII A'CryG_T

Cuts at:      0      62     213 
   Size:         62     151
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     104     136     213 
   Size:        104      32      77

  Fragments arranged by size: 

                 104      77      32
 
BanII G_rGCy'C

Cuts at:      0      59     213 
   Size:         59     154
 
BsaAI yAC'GTr

Cuts at:      0      63     213 
   Size:         63     150
 
BsaHI Gr'CG_yC

Cuts at:      0      77     213 
   Size:         77     136
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     104     134     213 
   Size:        104      30      79

  Fragments arranged by size: 

                 104      79      30
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      99     213 
   Size:         99     114
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     213 
   Size:         59     154
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      43     213 
   Size:         43     170
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      52     213 
   Size:         52     161
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      87     213 
   Size:         87     126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     123     155     213 
   Size:        123      32      58

  Fragments arranged by size: 

                 123      58      32
 
MslI CAynn'nnrTG

Cuts at:      0      50     213 
   Size:         50     163
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     213 
   Size:        104      32      77

  Fragments arranged by size: 

                 104      77      32
 
SfcI C'TryA_G

Cuts at:      0      16     165     213 
   Size:         16     149      48

  Fragments arranged by size: 

                 149      48      16
 
SspI AAT'ATT

Cuts at:      0     177     213 
   Size:        177      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     191     213 
   Size:        191      22
 

 Enzymes that do cut:

   AflIII     AloI    BanII    BsaAI    BsaHI    BsaXI    BsmBI Bsp1286I
    BspMI    BstXI     EciI    Hin4I     MslI     PpiI     SfcI     SspI
    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI