(Linear) (Six-base) MAPSORT of: KIEE6533.seq  Check: 5527  from: 1  to: 336



KIEE6533.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     161     336 
   Size:        161     175
 
AlwNI CAG_nnn'CTG

Cuts at:      0      55     336 
   Size:         55     281
 
AvaI C'yCGr_G

Cuts at:      0      62     336 
   Size:         62     274
 
BanII G_rGCy'C

Cuts at:      0      98     283     336 
   Size:         98     185      53

  Fragments arranged by size: 

                 185      98      53
 
Bme1580I G_kGCm'C

Cuts at:      0     114     336 
   Size:        114     222
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     108     336 
   Size:        108     228
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     336 
   Size:        103     233
 
BsaBI GATnn'nnATC

Cuts at:      0      13      73     336 
   Size:         13      60     263

  Fragments arranged by size: 

                 263      60      13
 
BseYI C'CCAG_C

Cuts at:      0     150     336 
   Size:        150     186
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     336 
   Size:        150     186
 
BsiHKAI G_wGCw'C

Cuts at:      0      98     199     336 
   Size:         98     101     137

  Fragments arranged by size: 

                 137     101      98
 
BsmI GAATG_Cn'

Cuts at:      0     138     336 
   Size:        138     198
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     114     199     283     336 
   Size:         98      16      85      84      53

  Fragments arranged by size: 

                  98      85      84      53      16
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     131     336 
   Size:        131     205
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     319     336 
   Size:        319      17
 
BstYI r'GATC_y

Cuts at:      0       8     336 
   Size:          8     328
 
BtgI C'CryG_G

Cuts at:      0     173     336 
   Size:        173     163
 
EaeI y'GGCC_r

Cuts at:      0     176     336 
   Size:        176     160
 
EcoICRI GAG'CTC

Cuts at:      0      96     336 
   Size:         96     240
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     336 
   Size:        103     233
 
MfeI C'AATT_G

Cuts at:      0     126     336 
   Size:        126     210
 
MscI TGG'CCA

Cuts at:      0     178     336 
   Size:        178     158
 
MslI CAynn'nnrTG

Cuts at:      0     143     172     336 
   Size:        143      29     164

  Fragments arranged by size: 

                 164     143      29
 
MspA1I CmG'CkG

Cuts at:      0     164     336 
   Size:        164     172
 
NcoI C'CATG_G

Cuts at:      0     173     336 
   Size:        173     163
 
SacI G_AGCT'C

Cuts at:      0      98     336 
   Size:         98     238
 
SmaI CCC'GGG

Cuts at:      0      64     336 
   Size:         64     272
 
StyI C'CwwG_G

Cuts at:      0     173     336 
   Size:        173     163
 
XmaI C'CCGG_G

Cuts at:      0      62     336 
   Size:         62     274
 

 Enzymes that do cut:

     AlfI    AlwNI     AvaI    BanII Bme1580I     BmrI     BpmI    BsaBI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I   BstAPI    BstXI    BstYI
     BtgI     EaeI  EcoICRI  Eco57MI     MfeI     MscI     MslI   MspA1I
     NcoI     SacI     SmaI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI