Restriction map for
pFN21AE6528
(Linear) (Six-base) MAPSORT of: KIEE6528.seq Check: 3128 from: 1 to: 525
KIEE6528.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 95 435 525
Size: 95 340 90
Fragments arranged by size:
340 95 90
ApoI r'AATT_y
Cuts at: 0 362 525
Size: 362 163
BamHI G'GATC_C
Cuts at: 0 395 525
Size: 395 130
BanII G_rGCy'C
Cuts at: 0 317 480 525
Size: 317 163 45
Fragments arranged by size:
317 163 45
BbvCI CC'TCA_GC
Cuts at: 0 214 439 525
Size: 214 225 86
Fragments arranged by size:
225 214 86
BglII A'GATC_T
Cuts at: 0 275 525
Size: 275 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 525
Size: 403 122
Bpu10I CC'TnA_GC
Cuts at: 0 214 439 525
Size: 214 225 86
Fragments arranged by size:
225 214 86
BsiHKAI G_wGCw'C
Cuts at: 0 317 525
Size: 317 208
BsmI GAATG_Cn'
Cuts at: 0 58 525
Size: 58 467
Bsp1286I G_dGCh'C
Cuts at: 0 317 480 525
Size: 317 163 45
Fragments arranged by size:
317 163 45
BsrBI CCG'CTC
Cuts at: 0 119 230 525
Size: 119 111 295
Fragments arranged by size:
295 119 111
BsrDI GCAATG_nn'
Cuts at: 0 208 525
Size: 208 317
BstAPI GCAn_nnn'nTGC
Cuts at: 0 435 525
Size: 435 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 111 525
Size: 111 414
BstYI r'GATC_y
Cuts at: 0 275 395 525
Size: 275 120 130
Fragments arranged by size:
275 130 120
EcoICRI GAG'CTC
Cuts at: 0 315 525
Size: 315 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 525
Size: 403 122
EcoO109I rG'GnC_Cy
Cuts at: 0 29 525
Size: 29 496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 30 525
Size: 30 495
HaeII r_GCGC'y
Cuts at: 0 144 263 525
Size: 144 119 262
Fragments arranged by size:
262 144 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 417 449 525
Size: 316 32 69 32 76
Fragments arranged by size:
316 76 69 32 32
MslI CAynn'nnrTG
Cuts at: 0 109 525
Size: 109 416
MspA1I CmG'CkG
Cuts at: 0 95 392 525
Size: 95 297 133
Fragments arranged by size:
297 133 95
PshAI GACnn'nnGTC
Cuts at: 0 306 326 525
Size: 306 20 199
Fragments arranged by size:
306 199 20
PvuII CAG'CTG
Cuts at: 0 392 525
Size: 392 133
SacI G_AGCT'C
Cuts at: 0 317 525
Size: 317 208
ScaI AGT'ACT
Cuts at: 0 166 525
Size: 166 359
SfcI C'TryA_G
Cuts at: 0 417 525
Size: 417 108
TatI w'GTAC_w
Cuts at: 0 164 452 525
Size: 164 288 73
Fragments arranged by size:
288 164 73
Enzymes that do cut:
AlwNI ApoI BamHI BanII BbvCI BglII BpmI Bpu10I
BsiHKAI BsmI Bsp1286I BsrBI BsrDI BstAPI BstXI BstYI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I MslI MspA1I
PshAI PvuII SacI ScaI SfcI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI
BbsI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI