(Linear) (Six-base) MAPSORT of: KIEE6524.seq  Check: 9156  from: 1  to: 390



KIEE6524.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     162     390 
   Size:        162     228
 
AhdI GACnn_n'nnGTC

Cuts at:      0     268     390 
   Size:        268     122
 
AleI CACnn'nnGTG

Cuts at:      0     204     390 
   Size:        204     186
 
AlwNI CAG_nnn'CTG

Cuts at:      0      14     390 
   Size:         14     376
 
ApaI G_GGCC'C

Cuts at:      0     183     390 
   Size:        183     207
 
AvaI C'yCGr_G

Cuts at:      0     159     390 
   Size:        159     231
 
BanII G_rGCy'C

Cuts at:      0     183     390 
   Size:        183     207
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     363     390 
   Size:        363      27
 
BglII A'GATC_T

Cuts at:      0     131     390 
   Size:        131     259
 
Bme1580I G_kGCm'C

Cuts at:      0     183     390 
   Size:        183     207
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     346     390 
   Size:        113     233      44

  Fragments arranged by size: 

                 233     113      44
 
BsaWI w'CCGG_w

Cuts at:      0     168     390 
   Size:        168     222
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       4      34     390 
   Size:          4      30     356

  Fragments arranged by size: 

                 356      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     177     390 
   Size:        177     213
 
BseYI C'CCAG_C

Cuts at:      0     182     390 
   Size:        182     208
 
Bsp1286I G_dGCh'C

Cuts at:      0     183     390 
   Size:        183     207
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     162     390 
   Size:        162     228
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      14     390 
   Size:         14     376
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     314     390 
   Size:        314      76
 
BstYI r'GATC_y

Cuts at:      0     131     390 
   Size:        131     259
 
BtsI GCAGTG_nn'

Cuts at:      0      12     390 
   Size:         12     378
 
EaeI y'GGCC_r

Cuts at:      0     316     390 
   Size:        316      74
 
EarI CTCTTCn'nnn_

Cuts at:      0      68     390 
   Size:         68     322
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     346     390 
   Size:        113     233      44

  Fragments arranged by size: 

                 233     113      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     179     390 
   Size:        179     211
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     254     286     390 
   Size:        254      32     104

  Fragments arranged by size: 

                 254     104      32
 
MscI TGG'CCA

Cuts at:      0     318     390 
   Size:        318      72
 
MslI CAynn'nnrTG

Cuts at:      0     204     390 
   Size:        204     186
 
PfoI T'CCnGG_A

Cuts at:      0      90     390 
   Size:         90     300
 
PspOMI G'GGCC_C

Cuts at:      0     179     390 
   Size:        179     211
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     216     390 
   Size:        216     174
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       4      36     390 
   Size:          4      32     354

  Fragments arranged by size: 

                 354      32       4
 

 Enzymes that do cut:

     AarI     AhdI     AleI    AlwNI     ApaI     AvaI    BanII     BbsI
    BglII Bme1580I     BpmI    BsaWI    BsaXI    BseRI    BseYI Bsp1286I
    BspMI   BstAPI    BstXI    BstYI     BtsI     EaeI     EarI  Eco57MI
 EcoO109I    Hin4I     MscI     MslI     PfoI   PspOMI    TaqII     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI