(Linear) (Six-base) MAPSORT of: KIEE6520.seq  Check: 7129  from: 1  to: 162



KIEE6520.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

BamHI G'GATC_C

Cuts at:      0      29     162 
   Size:         29     133
 
BstYI r'GATC_y

Cuts at:      0      29      78     162 
   Size:         29      49      84

  Fragments arranged by size: 

                  84      49      29
 
EaeI y'GGCC_r

Cuts at:      0     138     162 
   Size:        138      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      24     162 
   Size:         24     138
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      21      53     162 
   Size:         21      32     109

  Fragments arranged by size: 

                 109      32      21
 
MscI TGG'CCA

Cuts at:      0     140     162 
   Size:        140      22
 
MspA1I CmG'CkG

Cuts at:      0     114     162 
   Size:        114      48
 
PvuII CAG'CTG

Cuts at:      0     114     162 
   Size:        114      48
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     133     162 
   Size:        133      29
 

 Enzymes that do cut:

    BamHI    BstYI     EaeI    EcoNI    Hin4I     MscI   MspA1I    PvuII
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI