(Linear) (Six-base) MAPSORT of: KIEE6518.seq  Check: 7446  from: 1  to: 585



KIEE6518.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AleI CACnn'nnGTG

Cuts at:      0     195     585 
   Size:        195     390
 
BanI G'GyrC_C

Cuts at:      0     382     585 
   Size:        382     203
 
BanII G_rGCy'C

Cuts at:      0      23     311     585 
   Size:         23     288     274

  Fragments arranged by size: 

                 288     274      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     408     503     585 
   Size:        408      95      82

  Fragments arranged by size: 

                 408      95      82
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      94     128     585 
   Size:         94      34     457

  Fragments arranged by size: 

                 457      94      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      52     585 
   Size:         52     533
 
BlpI GC'TnA_GC

Cuts at:      0     452     585 
   Size:        452     133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     337     585 
   Size:         44     293     248

  Fragments arranged by size: 

                 293     248      44
 
Bpu10I CC'TnA_GC

Cuts at:      0      80      86     143     585 
   Size:         80       6      57     442

  Fragments arranged by size: 

                 442      80      57       6
 
BsaAI yAC'GTr

Cuts at:      0     465     585 
   Size:        465     120
 
BsaBI GATnn'nnATC

Cuts at:      0     208     585 
   Size:        208     377
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121     585 
   Size:         91      30     464

  Fragments arranged by size: 

                 464      91      30
 
BseYI C'CCAG_C

Cuts at:      0     246     477     520     585 
   Size:        246     231      43      65

  Fragments arranged by size: 

                 246     231      65      43
 
BsiEI CG_ry'CG

Cuts at:      0     186     585 
   Size:        186     399
 
BsiHKAI G_wGCw'C

Cuts at:      0     278     311     585 
   Size:        278      33     274

  Fragments arranged by size: 

                 278     274      33
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     278     311     585 
   Size:         23     255      33     274

  Fragments arranged by size: 

                 274     255      33      23
 
BsrDI GCAATG_nn'

Cuts at:      0     438     585 
   Size:        438     147
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     324     585 
   Size:        324     261
 
Bsu36I CC'TnA_GG

Cuts at:      0      95     585 
   Size:         95     490
 
BtsI GCAGTG_nn'

Cuts at:      0     229     585 
   Size:        229     356
 
EaeI y'GGCC_r

Cuts at:      0     470     585 
   Size:        470     115
 
EarI CTCTTCn'nnn_

Cuts at:      0     149     299     585 
   Size:        149     150     286

  Fragments arranged by size: 

                 286     150     149
 
EcoICRI GAG'CTC

Cuts at:      0     309     585 
   Size:        309     276
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     337     585 
   Size:         44     293     248

  Fragments arranged by size: 

                 293     248      44
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     186     585 
   Size:        186     399
 
HaeII r_GCGC'y

Cuts at:      0      93     247     585 
   Size:         93     154     338

  Fragments arranged by size: 

                 338     154      93
 
MscI TGG'CCA

Cuts at:      0     472     585 
   Size:        472     113
 
MslI CAynn'nnrTG

Cuts at:      0     195     442     585 
   Size:        195     247     143

  Fragments arranged by size: 

                 247     195     143
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     468     585 
   Size:        468     117
 
PmlI CAC'GTG

Cuts at:      0     465     585 
   Size:        465     120
 
SacI G_AGCT'C

Cuts at:      0     311     585 
   Size:        311     274
 
SapI GCTCTTCn'nnn_

Cuts at:      0     299     585 
   Size:        299     286
 
StyI C'CwwG_G

Cuts at:      0      38      50     585 
   Size:         38      12     535

  Fragments arranged by size: 

                 535      38      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     207     436     469     585 
   Size:        207     229      33     116

  Fragments arranged by size: 

                 229     207     116      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     562     585 
   Size:        562      23
 

 Enzymes that do cut:

     AleI     BanI    BanII     BbsI     BcgI     BglI     BlpI     BpmI
   Bpu10I    BsaAI    BsaBI    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BsrDI    BstXI   Bsu36I     BtsI     EaeI     EarI  EcoICRI  Eco57MI
     FalI    HaeII     MscI     MslI    PflMI     PmlI     SacI     SapI
     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaHI    BsaWI    BseRI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI