(Linear) (Six-base) MAPSORT of: KIEE6516.seq  Check: 4321  from: 1  to: 555



KIEE6516.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     489     555 
   Size:        489      66
 
AlwNI CAG_nnn'CTG

Cuts at:      0     126     555 
   Size:        126     429
 
ApoI r'AATT_y

Cuts at:      0     274     456     555 
   Size:        274     182      99

  Fragments arranged by size: 

                 274     182      99
 
BanI G'GyrC_C

Cuts at:      0     496     555 
   Size:        496      59
 
BanII G_rGCy'C

Cuts at:      0     554     555 
   Size:        554       1
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     501     535     555 
   Size:        501      34      20

  Fragments arranged by size: 

                 501      34      20
 
BclI T'GATC_A

Cuts at:      0     314     555 
   Size:        314     241
 
BglI GCCn_nnn'nGGC

Cuts at:      0     529     555 
   Size:        529      26
 
Bme1580I G_kGCm'C

Cuts at:      0     410     555 
   Size:        410     145
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     446     555 
   Size:        446     109
 
BsaWI w'CCGG_w

Cuts at:      0     283     555 
   Size:        283     272
 
BseYI C'CCAG_C

Cuts at:      0     116     555 
   Size:        116     439
 
BsiHKAI G_wGCw'C

Cuts at:      0     399     554     555 
   Size:        399     155       1

  Fragments arranged by size: 

                 399     155       1
 
BsmI GAATG_Cn'

Cuts at:      0       7     555 
   Size:          7     548
 
Bsp1286I G_dGCh'C

Cuts at:      0     399     410     554     555 
   Size:        399      11     144       1

  Fragments arranged by size: 

                 399     144      11       1
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      23     489     555 
   Size:         23     466      66

  Fragments arranged by size: 

                 466      66      23
 
BstYI r'GATC_y

Cuts at:      0     216     555 
   Size:        216     339
 
Bsu36I CC'TnA_GG

Cuts at:      0      72     555 
   Size:         72     483
 
BtsI GCAGTG_nn'

Cuts at:      0     498     519     555 
   Size:        498      21      36

  Fragments arranged by size: 

                 498      36      21
 
DraIII CAC_nnn'GTG

Cuts at:      0     312     369     555 
   Size:        312      57     186

  Fragments arranged by size: 

                 312     186      57
 
EcoICRI GAG'CTC

Cuts at:      0     552     555 
   Size:        552       3
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     126     555 
   Size:        126     429
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     457     555 
   Size:        457      98
 
PfoI T'CCnGG_A

Cuts at:      0     219     555 
   Size:        219     336
 
PstI C_TGCA'G

Cuts at:      0     152     555 
   Size:        152     403
 
SacI G_AGCT'C

Cuts at:      0     554     555 
   Size:        554       1
 
SfcI C'TryA_G

Cuts at:      0     148     555 
   Size:        148     407
 
StyI C'CwwG_G

Cuts at:      0     402     555 
   Size:        402     153
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     193     555 
   Size:        193     362
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     168     200     555 
   Size:        168      32     355

  Fragments arranged by size: 

                 355     168      32
 

 Enzymes that do cut:

     AarI    AlwNI     ApoI     BanI    BanII     BcgI     BclI     BglI
 Bme1580I     BmrI    BsaWI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI
    BstYI   Bsu36I     BtsI   DraIII  EcoICRI     FalI     MmeI     PfoI
     PstI     SacI     SfcI     StyI    TaqII     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI    BciVI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI