(Linear) (Six-base) MAPSORT of: KIEE6514.seq  Check: 2376  from: 1  to: 441



KIEE6514.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AccI GT'mk_AC

Cuts at:      0     120     441 
   Size:        120     321
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     441 
   Size:        130     311
 
AleI CACnn'nnGTG

Cuts at:      0     415     441 
   Size:        415      26
 
BglI GCCn_nnn'nGGC

Cuts at:      0     349     441 
   Size:        349      92
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      19     441 
   Size:         19     422
 
BsiHKAI G_wGCw'C

Cuts at:      0     183     410     441 
   Size:        183     227      31

  Fragments arranged by size: 

                 227     183      31
 
BsmI GAATG_Cn'

Cuts at:      0     170     441 
   Size:        170     271
 
Bsp1286I G_dGCh'C

Cuts at:      0     183     410     441 
   Size:        183     227      31

  Fragments arranged by size: 

                 227     183      31
 
BsrDI GCAATG_nn'

Cuts at:      0     244     441 
   Size:        244     197
 
BtgI C'CryG_G

Cuts at:      0     320     441 
   Size:        320     121
 
BtsI GCAGTG_nn'

Cuts at:      0     256     410     430     441 
   Size:        256     154      20      11

  Fragments arranged by size: 

                 256     154      20      11
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     441 
   Size:        130     311
 
HincII GTy'rAC

Cuts at:      0     219     441 
   Size:        219     222
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     368     441 
   Size:        239     129      73

  Fragments arranged by size: 

                 239     129      73
 
MslI CAynn'nnrTG

Cuts at:      0      34     249     415     441 
   Size:         34     215     166      26

  Fragments arranged by size: 

                 215     166      34      26
 
NcoI C'CATG_G

Cuts at:      0     320     441 
   Size:        320     121
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     348     380     441 
   Size:        348      32      61

  Fragments arranged by size: 

                 348      61      32
 
PstI C_TGCA'G

Cuts at:      0     425     441 
   Size:        425      16
 
SfcI C'TryA_G

Cuts at:      0     121     421     441 
   Size:        121     300      20

  Fragments arranged by size: 

                 300     121      20
 
StyI C'CwwG_G

Cuts at:      0     320     441 
   Size:        320     121
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     345     441 
   Size:        345      96
 

 Enzymes that do cut:

     AccI     AcuI     AleI     BglI     BsaI  BsiHKAI     BsmI Bsp1286I
    BsrDI     BtgI     BtsI  Eco57MI   HincII     MmeI     MslI     NcoI
     PpiI     PstI     SfcI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI