(Linear) (Six-base) MAPSORT of: KIEE6512.seq  Check: 6225  from: 1  to: 489



KIEE6512.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     489 
   Size:        115     374
 
AfeI AGC'GCT

Cuts at:      0     199     489 
   Size:        199     290
 
AflIII A'CryG_T

Cuts at:      0      81     489 
   Size:         81     408
 
AlwNI CAG_nnn'CTG

Cuts at:      0      60     297     489 
   Size:         60     237     192

  Fragments arranged by size: 

                 237     192      60
 
BanI G'GyrC_C

Cuts at:      0     244     489 
   Size:        244     245
 
BanII G_rGCy'C

Cuts at:      0     452     489 
   Size:        452      37
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     388     489 
   Size:        388     101
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     489 
   Size:        445      44
 
BsaHI Gr'CG_yC

Cuts at:      0     262     362     489 
   Size:        262     100     127

  Fragments arranged by size: 

                 262     127     100
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     139     169     370     400     489 
   Size:        139      30     201      30      89

  Fragments arranged by size: 

                 201     139      89      30      30
 
BseYI C'CCAG_C

Cuts at:      0     173     489 
   Size:        173     316
 
Bsp1286I G_dGCh'C

Cuts at:      0     452     489 
   Size:        452      37
 
BspHI T'CATG_A

Cuts at:      0     281     489 
   Size:        281     208
 
BstEII G'GTnAC_C

Cuts at:      0     231     489 
   Size:        231     258
 
BstYI r'GATC_y

Cuts at:      0     401     489 
   Size:        401      88
 
BtgI C'CryG_G

Cuts at:      0     470     489 
   Size:        470      19
 
BtsI GCAGTG_nn'

Cuts at:      0     305     489 
   Size:        305     184
 
EaeI y'GGCC_r

Cuts at:      0     395     489 
   Size:        395      94
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     489 
   Size:         19     470
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     388     489 
   Size:        115     273     101

  Fragments arranged by size: 

                 273     115     101
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      52     489 
   Size:         52     437
 
EcoO109I rG'GnC_Cy

Cuts at:      0      57     489 
   Size:         57     432
 
HaeII r_GCGC'y

Cuts at:      0     117     201     384     489 
   Size:        117      84     183     105

  Fragments arranged by size: 

                 183     117     105      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     370     402     489 
   Size:        370      32      87

  Fragments arranged by size: 

                 370      87      32
 
MluI A'CGCG_T

Cuts at:      0      81     489 
   Size:         81     408
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     368     489 
   Size:        368     121
 
MscI TGG'CCA

Cuts at:      0     397     489 
   Size:        397      92
 
MspA1I CmG'CkG

Cuts at:      0      30      42     145     183     294     489 
   Size:         30      12     103      38     111     195

  Fragments arranged by size: 

                 195     111     103      38      30      12
 
NspI r_CATG'y

Cuts at:      0      39     489 
   Size:         39     450
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     297     317     347     489 
   Size:        297      20      30     142

  Fragments arranged by size: 

                 297     142      30      20
 
PfoI T'CCnGG_A

Cuts at:      0      47     489 
   Size:         47     442
 
PstI C_TGCA'G

Cuts at:      0     140     489 
   Size:        140     349
 
PvuII CAG'CTG

Cuts at:      0      30     294     489 
   Size:         30     264     195

  Fragments arranged by size: 

                 264     195      30
 
SfcI C'TryA_G

Cuts at:      0     136     187     489 
   Size:        136      51     302

  Fragments arranged by size: 

                 302     136      51
 
SmlI C'TyrA_G

Cuts at:      0     460     489 
   Size:        460      29
 
StuI AGG'CCT

Cuts at:      0     466     489 
   Size:        466      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     187     489 
   Size:        187     302
 
TatI w'GTAC_w

Cuts at:      0     416     489 
   Size:        416      73
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII    AlwNI     BanI    BanII     BpmI    BpuEI
    BsaHI    BsaXI    BseYI Bsp1286I    BspHI   BstEII    BstYI     BtgI
     BtsI     EaeI     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
     MluI     MmeI     MscI   MspA1I     NspI    PflMI     PfoI     PstI
    PvuII     SfcI     SmlI     StuI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI