(Linear) (Six-base) MAPSORT of: KIEE6509.seq  Check: 4330  from: 1  to: 528



KIEE6509.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     378     528 
   Size:        378     150
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     528 
   Size:         36     492
 
AhdI GACnn_n'nnGTC

Cuts at:      0     466     528 
   Size:        466      62
 
AvaI C'yCGr_G

Cuts at:      0     165     528 
   Size:        165     363
 
BanII G_rGCy'C

Cuts at:      0     416     528 
   Size:        416     112
 
BglI GCCn_nnn'nGGC

Cuts at:      0     151     337     528 
   Size:        151     186     191

  Fragments arranged by size: 

                 191     186     151
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     435     528 
   Size:        435      93
 
Bpu10I CC'TnA_GC

Cuts at:      0      13     528 
   Size:         13     515
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     528 
   Size:        409     119
 
BsaWI w'CCGG_w

Cuts at:      0     107     528 
   Size:        107     421
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     481     528 
   Size:        481      47
 
BseYI C'CCAG_C

Cuts at:      0     121     528 
   Size:        121     407
 
BsiHKAI G_wGCw'C

Cuts at:      0     395     416     491     528 
   Size:        395      21      75      37

  Fragments arranged by size: 

                 395      75      37      21
 
Bsp1286I G_dGCh'C

Cuts at:      0     395     416     491     528 
   Size:        395      21      75      37

  Fragments arranged by size: 

                 395      75      37      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     378     528 
   Size:        378     150
 
BsrDI GCAATG_nn'

Cuts at:      0     429     528 
   Size:        429      99
 
BsrFI r'CCGG_y

Cuts at:      0     316     528 
   Size:        316     212
 
BstEII G'GTnAC_C

Cuts at:      0     468     528 
   Size:        468      60
 
BtsI GCAGTG_nn'

Cuts at:      0     125     528 
   Size:        125     403
 
EaeI y'GGCC_r

Cuts at:      0      29     528 
   Size:         29     499
 
EarI CTCTTCn'nnn_

Cuts at:      0      55     528 
   Size:         55     473
 
EcoICRI GAG'CTC

Cuts at:      0     414     528 
   Size:        414     114
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     528 
   Size:         36     492
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     528 
   Size:        114     414
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     161     193     452     484     528 
   Size:        161      32     259      32      44

  Fragments arranged by size: 

                 259     161      44      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     528 
   Size:        425     103
 
MscI TGG'CCA

Cuts at:      0      31     528 
   Size:         31     497
 
NspI r_CATG'y

Cuts at:      0      45     528 
   Size:         45     483
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     245     528 
   Size:        245     283
 
PfoI T'CCnGG_A

Cuts at:      0     407     528 
   Size:        407     121
 
SacI G_AGCT'C

Cuts at:      0     416     528 
   Size:        416     112
 
SapI GCTCTTCn'nnn_

Cuts at:      0      55     528 
   Size:         55     473
 
SmaI CCC'GGG

Cuts at:      0     167     528 
   Size:        167     361
 
SmlI C'TyrA_G

Cuts at:      0     424     528 
   Size:        424     104
 
SphI G_CATG'C

Cuts at:      0      45     528 
   Size:         45     483
 
StyI C'CwwG_G

Cuts at:      0     299     528 
   Size:        299     229
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     349     528 
   Size:        349     179
 
XmaI C'CCGG_G

Cuts at:      0     165     528 
   Size:        165     363
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AvaI    BanII     BglI     BplI   Bpu10I
    BpuEI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrDI
    BsrFI   BstEII     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    Hin4I     MmeI     MscI     NspI    PflMI     PfoI     SacI     SapI
     SmaI     SmlI     SphI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI