(Linear) (Six-base) MAPSORT of: KIEE6505.seq  Check: 8249  from: 1  to: 600



KIEE6505.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     261     523     600 
   Size:        261     262      77

  Fragments arranged by size: 

                 262     261      77
 
AvaI C'yCGr_G

Cuts at:      0     473     600 
   Size:        473     127
 
BamHI G'GATC_C

Cuts at:      0     100     600 
   Size:        100     500
 
BanI G'GyrC_C

Cuts at:      0     281     600 
   Size:        281     319
 
BanII G_rGCy'C

Cuts at:      0     575     600 
   Size:        575      25
 
BbvCI CC'TCA_GC

Cuts at:      0      35     540     600 
   Size:         35     505      60

  Fragments arranged by size: 

                 505      60      35
 
BclI T'GATC_A

Cuts at:      0      15     600 
   Size:         15     585
 
BfrBI ATG'CAT

Cuts at:      0      11     600 
   Size:         11     589
 
Bme1580I G_kGCm'C

Cuts at:      0     286     600 
   Size:        286     314
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     600 
   Size:        484     116
 
Bpu10I CC'TnA_GC

Cuts at:      0      35     452     540     600 
   Size:         35     417      88      60

  Fragments arranged by size: 

                 417      88      60      35
 
BseYI C'CCAG_C

Cuts at:      0     193     523     600 
   Size:        193     330      77

  Fragments arranged by size: 

                 330     193      77
 
BsmI GAATG_Cn'

Cuts at:      0      13     593     600 
   Size:         13     580       7

  Fragments arranged by size: 

                 580      13       7
 
Bsp1286I G_dGCh'C

Cuts at:      0     286     575     600 
   Size:        286     289      25

  Fragments arranged by size: 

                 289     286      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     269     600 
   Size:        269     331
 
BstEII G'GTnAC_C

Cuts at:      0      85     600 
   Size:         85     515
 
BstYI r'GATC_y

Cuts at:      0     100     146     600 
   Size:        100      46     454

  Fragments arranged by size: 

                 454     100      46
 
BtgI C'CryG_G

Cuts at:      0     236     443     600 
   Size:        236     207     157

  Fragments arranged by size: 

                 236     207     157
 
EaeI y'GGCC_r

Cuts at:      0     233     600 
   Size:        233     367
 
EarI CTCTTCn'nnn_

Cuts at:      0     514     600 
   Size:        514      86
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     600 
   Size:        484     116
 
EcoO109I rG'GnC_Cy

Cuts at:      0     258     600 
   Size:        258     342
 
MscI TGG'CCA

Cuts at:      0     235     600 
   Size:        235     365
 
MslI CAynn'nnrTG

Cuts at:      0     401     600 
   Size:        401     199
 
MspA1I CmG'CkG

Cuts at:      0     193     250     600 
   Size:        193      57     350

  Fragments arranged by size: 

                 350     193      57
 
NcoI C'CATG_G

Cuts at:      0     236     600 
   Size:        236     364
 
NsiI A_TGCA'T

Cuts at:      0      13     600 
   Size:         13     587
 
PasI CC'CwG_GG

Cuts at:      0     130     600 
   Size:        130     470
 
PpuMI rG'GwC_Cy

Cuts at:      0     258     600 
   Size:        258     342
 
PstI C_TGCA'G

Cuts at:      0     266     600 
   Size:        266     334
 
PvuII CAG'CTG

Cuts at:      0     193     250     600 
   Size:        193      57     350

  Fragments arranged by size: 

                 350     193      57
 
SfcI C'TryA_G

Cuts at:      0      53     262     600 
   Size:         53     209     338

  Fragments arranged by size: 

                 338     209      53
 
StyI C'CwwG_G

Cuts at:      0     236     600 
   Size:        236     364
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     104     600 
   Size:        104     496
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     441     600 
   Size:        441     159
 
XmnI GAAnn'nnTTC

Cuts at:      0     371     600 
   Size:        371     229
 

 Enzymes that do cut:

    AlwNI     AvaI    BamHI     BanI    BanII    BbvCI     BclI    BfrBI
 Bme1580I     BpmI   Bpu10I    BseYI     BsmI Bsp1286I    BspMI   BstEII
    BstYI     BtgI     EaeI     EarI  Eco57MI EcoO109I     MscI     MslI
   MspA1I     NcoI     NsiI     PasI    PpuMI     PstI    PvuII     SfcI
     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI
     BcgI    BciVI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI