Restriction map for
pFN21AE6504
(Linear) (Six-base) MAPSORT of: KIEE6504.seq Check: 772 from: 1 to: 537
KIEE6504.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 466 537
Size: 466 71
ApaI G_GGCC'C
Cuts at: 0 167 537
Size: 167 370
ApoI r'AATT_y
Cuts at: 0 12 537
Size: 12 525
AvaI C'yCGr_G
Cuts at: 0 90 122 219 537
Size: 90 32 97 318
Fragments arranged by size:
318 97 90 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 123 156 519 537
Size: 123 33 363 18
Fragments arranged by size:
363 123 33 18
BanII G_rGCy'C
Cuts at: 0 167 537
Size: 167 370
BglI GCCn_nnn'nGGC
Cuts at: 0 162 537
Size: 162 375
Bme1580I G_kGCm'C
Cuts at: 0 167 537
Size: 167 370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 537
Size: 255 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 242 537
Size: 242 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 247 277 537
Size: 247 30 260
Fragments arranged by size:
260 247 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 20 68 537
Size: 20 48 469
Fragments arranged by size:
469 48 20
BseYI C'CCAG_C
Cuts at: 0 290 537
Size: 290 247
BsiEI CG_ry'CG
Cuts at: 0 112 537
Size: 112 425
BsiWI C'GTAC_G
Cuts at: 0 158 179 537
Size: 158 21 358
Fragments arranged by size:
358 158 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 119 537
Size: 119 418
Bsp1286I G_dGCh'C
Cuts at: 0 167 537
Size: 167 370
BsrFI r'CCGG_y
Cuts at: 0 227 537
Size: 227 310
BstYI r'GATC_y
Cuts at: 0 438 537
Size: 438 99
BtgI C'CryG_G
Cuts at: 0 106 281 537
Size: 106 175 256
Fragments arranged by size:
256 175 106
EaeI y'GGCC_r
Cuts at: 0 109 537
Size: 109 428
EagI C'GGCC_G
Cuts at: 0 109 537
Size: 109 428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 116 537
Size: 116 421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 537
Size: 255 282
EcoNI CCTnn'n_nnAGG
Cuts at: 0 270 537
Size: 270 267
EcoO109I rG'GnC_Cy
Cuts at: 0 164 275 287 537
Size: 164 111 12 250
Fragments arranged by size:
250 164 111 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 537
Size: 386 151
MspA1I CmG'CkG
Cuts at: 0 283 537
Size: 283 254
PasI CC'CwG_GG
Cuts at: 0 313 336 537
Size: 313 23 201
Fragments arranged by size:
313 201 23
PpuMI rG'GwC_Cy
Cuts at: 0 275 287 537
Size: 275 12 250
Fragments arranged by size:
275 250 12
PspOMI G'GGCC_C
Cuts at: 0 163 537
Size: 163 374
SacII CC_GC'GG
Cuts at: 0 284 537
Size: 284 253
SanDI GG'GwC_CC
Cuts at: 0 287 537
Size: 287 250
SfcI C'TryA_G
Cuts at: 0 467 537
Size: 467 70
SmaI CCC'GGG
Cuts at: 0 92 221 537
Size: 92 129 316
Fragments arranged by size:
316 129 92
SmlI C'TyrA_G
Cuts at: 0 122 537
Size: 122 415
StyI C'CwwG_G
Cuts at: 0 346 442 537
Size: 346 96 95
Fragments arranged by size:
346 96 95
XhoI C'TCGA_G
Cuts at: 0 122 537
Size: 122 415
XmaI C'CCGG_G
Cuts at: 0 90 219 537
Size: 90 129 318
Fragments arranged by size:
318 129 90
Enzymes that do cut:
AlwNI ApaI ApoI AvaI BaeI BanII BglI Bme1580I
BpmI BsaI BsaXI BseRI BseYI BsiEI BsiWI BsmBI
Bsp1286I BsrFI BstYI BtgI EaeI EagI EciI Eco57MI
EcoNI EcoO109I MmeI MspA1I PasI PpuMI PspOMI SacII
SanDI SfcI SmaI SmlI StyI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsgI BsiHKAI BsmI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmnI ZraI