(Linear) (Six-base) MAPSORT of: KIEE6502.seq  Check: 9972  from: 1  to: 621



KIEE6502.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     565     621 
   Size:        245     320      56

  Fragments arranged by size: 

                 320     245      56
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     555     621 
   Size:        555      66
 
ApoI r'AATT_y

Cuts at:      0     484     621 
   Size:        484     137
 
AseI AT'TA_AT

Cuts at:      0     200     621 
   Size:        200     421
 
BanI G'GyrC_C

Cuts at:      0     106     621 
   Size:        106     515
 
BanII G_rGCy'C

Cuts at:      0      23     221     337     621 
   Size:         23     198     116     284

  Fragments arranged by size: 

                 284     198     116      23
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     573     621 
   Size:        573      48
 
BsaWI w'CCGG_w

Cuts at:      0     122     315     621 
   Size:        122     193     306

  Fragments arranged by size: 

                 306     193     122
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     363     393     621 
   Size:        363      30     228

  Fragments arranged by size: 

                 363     228      30
 
BseYI C'CCAG_C

Cuts at:      0     602     621 
   Size:        602      19
 
BsiEI CG_ry'CG

Cuts at:      0     123     621 
   Size:        123     498
 
BsiHKAI G_wGCw'C

Cuts at:      0      23     337     621 
   Size:         23     314     284

  Fragments arranged by size: 

                 314     284      23
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     139     621 
   Size:        139     482
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     221     337     621 
   Size:         23     198     116     284

  Fragments arranged by size: 

                 284     198     116      23
 
DraI TTT'AAA

Cuts at:      0     171     621 
   Size:        171     450
 
EarI CTCTTCn'nnn_

Cuts at:      0     209     621 
   Size:        209     412
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     621 
   Size:         19     602
 
EcoICRI GAG'CTC

Cuts at:      0      21     335     621 
   Size:         21     314     286

  Fragments arranged by size: 

                 314     286      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     565     573     621 
   Size:        245     320       8      48

  Fragments arranged by size: 

                 320     245      48       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0       9     621 
   Size:          9     612
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     202     621 
   Size:        202     419
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     576     608     621 
   Size:        576      32      13

  Fragments arranged by size: 

                 576      32      13
 
NdeI CA'TA_TG

Cuts at:      0     534     621 
   Size:        534      87
 
NspI r_CATG'y

Cuts at:      0     380     480     621 
   Size:        380     100     141

  Fragments arranged by size: 

                 380     141     100
 
PpuMI rG'GwC_Cy

Cuts at:      0       9     621 
   Size:          9     612
 
SacI G_AGCT'C

Cuts at:      0      23     337     621 
   Size:         23     314     284

  Fragments arranged by size: 

                 314     284      23
 
SapI GCTCTTCn'nnn_

Cuts at:      0     209     621 
   Size:        209     412
 
SspI AAT'ATT

Cuts at:      0     363     621 
   Size:        363     258
 
StuI AGG'CCT

Cuts at:      0     296     621 
   Size:        296     325
 
StyI C'CwwG_G

Cuts at:      0     134     297     324     621 
   Size:        134     163      27     297

  Fragments arranged by size: 

                 297     163     134      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     124     621 
   Size:        124     497
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     363     395     433     465     621 
   Size:        363      32      38      32     156

  Fragments arranged by size: 

                 363     156      38      32      32
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     AseI     BanI    BanII     BpmI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I     DraI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I     NdeI     NspI
    PpuMI     SacI     SapI     SspI     StuI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BseRI     BsgI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SwaI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI