Restriction map for
pFN21AE6501
(Linear) (Six-base) MAPSORT of: KIEE6501.seq Check: 844 from: 1 to: 408
KIEE6501.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 255 287 408
Size: 255 32 121
Fragments arranged by size:
255 121 32
ApaI G_GGCC'C
Cuts at: 0 139 408
Size: 139 269
AvaI C'yCGr_G
Cuts at: 0 392 408
Size: 392 16
BamHI G'GATC_C
Cuts at: 0 26 267 408
Size: 26 241 141
Fragments arranged by size:
241 141 26
BanI G'GyrC_C
Cuts at: 0 168 408
Size: 168 240
BanII G_rGCy'C
Cuts at: 0 139 408
Size: 139 269
Bme1580I G_kGCm'C
Cuts at: 0 139 171 408
Size: 139 32 237
Fragments arranged by size:
237 139 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 136 200 408
Size: 136 64 208
Fragments arranged by size:
208 136 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 132 262 408
Size: 132 130 146
Fragments arranged by size:
146 132 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 257 408
Size: 237 20 151
Fragments arranged by size:
237 151 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 215 408
Size: 215 193
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 255 285 408
Size: 255 30 123
Fragments arranged by size:
255 123 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 263 385 408
Size: 121 142 122 23
Fragments arranged by size:
142 122 121 23
BseYI C'CCAG_C
Cuts at: 0 314 408
Size: 314 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 408
Size: 28 380
Bsp1286I G_dGCh'C
Cuts at: 0 139 171 408
Size: 139 32 237
Fragments arranged by size:
237 139 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 233 408
Size: 233 175
BsrDI GCAATG_nn'
Cuts at: 0 124 408
Size: 124 284
BsrFI r'CCGG_y
Cuts at: 0 303 408
Size: 303 105
BstEII G'GTnAC_C
Cuts at: 0 151 408
Size: 151 257
BstYI r'GATC_y
Cuts at: 0 26 186 267 408
Size: 26 160 81 141
Fragments arranged by size:
160 141 81 26
Bsu36I CC'TnA_GG
Cuts at: 0 131 347 365 408
Size: 131 216 18 43
Fragments arranged by size:
216 131 43 18
EaeI y'GGCC_r
Cuts at: 0 160 301 408
Size: 160 141 107
Fragments arranged by size:
160 141 107
EarI CTCTTCn'nnn_
Cuts at: 0 392 408
Size: 392 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 182 408
Size: 182 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 257 408
Size: 237 20 151
Fragments arranged by size:
237 151 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 161 408
Size: 161 247
EcoO109I rG'GnC_Cy
Cuts at: 0 135 136 310 408
Size: 135 1 174 98
Fragments arranged by size:
174 135 98 1
MscI TGG'CCA
Cuts at: 0 162 408
Size: 162 246
NaeI GCC'GGC
Cuts at: 0 305 408
Size: 305 103
NgoMIV G'CCGG_C
Cuts at: 0 303 408
Size: 303 105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 255 287 408
Size: 255 32 121
Fragments arranged by size:
255 121 32
PpuMI rG'GwC_Cy
Cuts at: 0 310 408
Size: 310 98
PspOMI G'GGCC_C
Cuts at: 0 135 408
Size: 135 273
SmaI CCC'GGG
Cuts at: 0 394 408
Size: 394 14
StyI C'CwwG_G
Cuts at: 0 157 298 408
Size: 157 141 110
Fragments arranged by size:
157 141 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 101 116 408
Size: 101 15 292
Fragments arranged by size:
292 101 15
TatI w'GTAC_w
Cuts at: 0 401 408
Size: 401 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 329 408
Size: 329 79
XmaI C'CCGG_G
Cuts at: 0 392 408
Size: 392 16
Enzymes that do cut:
AloI ApaI AvaI BamHI BanI BanII Bme1580I BmrI
BplI BpmI BsaI BsaXI BseRI BseYI BsgI Bsp1286I
BspMI BsrDI BsrFI BstEII BstYI Bsu36I EaeI EarI
EciI Eco57MI EcoNI EcoO109I MscI NaeI NgoMIV PpiI
PpuMI PspOMI SmaI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI MspA1I NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmnI ZraI