Restriction map for
pFN21AE6490
(Linear) (Six-base) MAPSORT of: KIEE6490.seq Check: 585 from: 1 to: 1776
KIEE6490.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 16 1776
Size: 16 1760
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1671 1776
Size: 1222 449 105
Fragments arranged by size:
1222 449 105
AflII C'TTAA_G
Cuts at: 0 247 1776
Size: 247 1529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 951 1776
Size: 951 825
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 775 807 1776
Size: 775 32 969
Fragments arranged by size:
969 775 32
AlwNI CAG_nnn'CTG
Cuts at: 0 21 1740 1776
Size: 21 1719 36
Fragments arranged by size:
1719 36 21
ApaI G_GGCC'C
Cuts at: 0 970 1776
Size: 970 806
ApaLI G'TGCA_C
Cuts at: 0 147 1416 1776
Size: 147 1269 360
Fragments arranged by size:
1269 360 147
AvaI C'yCGr_G
Cuts at: 0 1034 1610 1776
Size: 1034 576 166
Fragments arranged by size:
1034 576 166
BanI G'GyrC_C
Cuts at: 0 444 715 1776
Size: 444 271 1061
Fragments arranged by size:
1061 444 271
BanII G_rGCy'C
Cuts at: 0 536 970 1776
Size: 536 434 806
Fragments arranged by size:
806 536 434
BbsI GAAGACnn'nnnn_
Cuts at: 0 1237 1566 1776
Size: 1237 329 210
Fragments arranged by size:
1237 329 210
BbvCI CC'TCA_GC
Cuts at: 0 432 1776
Size: 432 1344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 483 517 987 1021 1431 1465 1776
Size: 483 34 470 34 410 34 311
Fragments arranged by size:
483 470 410 311 34 34 34
BclI T'GATC_A
Cuts at: 0 1646 1776
Size: 1646 130
BglI GCCn_nnn'nGGC
Cuts at: 0 25 1468 1776
Size: 25 1443 308
Fragments arranged by size:
1443 308 25
Bme1580I G_kGCm'C
Cuts at: 0 62 151 449 624 970 1420 1716 1776
Size: 62 89 298 175 346 450 296 60
Fragments arranged by size:
450 346 298 296 175 89 62 60
BmgBI CAC'GTC
Cuts at: 0 599 1776
Size: 599 1177
BmrI ACTGGGnnnn_n'
Cuts at: 0 89 648 1728 1776
Size: 89 559 1080 48
Fragments arranged by size:
1080 559 89 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1352 1776
Size: 520 832 424
Fragments arranged by size:
832 520 424
Bpu10I CC'TnA_GC
Cuts at: 0 432 1776
Size: 432 1344
BsaBI GATnn'nnATC
Cuts at: 0 211 1776
Size: 211 1565
BsaWI w'CCGG_w
Cuts at: 0 881 1776
Size: 881 895
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 775 805 1776
Size: 775 30 971
Fragments arranged by size:
971 775 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 520 1210 1615 1776
Size: 331 189 690 405 161
Fragments arranged by size:
690 405 331 189 161
BseYI C'CCAG_C
Cuts at: 0 963 1058 1272 1329 1670 1776
Size: 963 95 214 57 341 106
Fragments arranged by size:
963 341 214 106 95 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1497 1678 1776
Size: 460 1037 181 98
Fragments arranged by size:
1037 460 181 98
BsiEI CG_ry'CG
Cuts at: 0 1312 1776
Size: 1312 464
BsiHKAI G_wGCw'C
Cuts at: 0 151 536 956 1420 1776
Size: 151 385 420 464 356
Fragments arranged by size:
464 420 385 356 151
Bsp1286I G_dGCh'C
Cuts at: 0 62 151 449 536 624 956 970 1420
Size: 62 89 298 87 88 332 14 450
Cuts at: 1420 1716 1776
Size: 296 60
Fragments arranged by size:
450 332 298 296 89 88 87 62
60 14
BspEI T'CCGG_A
Cuts at: 0 881 1776
Size: 881 895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 320 1776
Size: 16 304 1456
Fragments arranged by size:
1456 304 16
BsrDI GCAATG_nn'
Cuts at: 0 1373 1776
Size: 1373 403
BstAPI GCAn_nnn'nTGC
Cuts at: 0 960 1359 1776
Size: 960 399 417
Fragments arranged by size:
960 417 399
BstEII G'GTnAC_C
Cuts at: 0 561 1776
Size: 561 1215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 297 1530 1776
Size: 297 1233 246
Fragments arranged by size:
1233 297 246
BstYI r'GATC_y
Cuts at: 0 857 1776
Size: 857 919
BtgI C'CryG_G
Cuts at: 0 818 1252 1466 1776
Size: 818 434 214 310
Fragments arranged by size:
818 434 310 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 276 309 435 684 1635 1776
Size: 276 33 126 249 951 141
Fragments arranged by size:
951 276 249 141 126 33
BtsI GCAGTG_nn'
Cuts at: 0 173 1308 1348 1776
Size: 173 1135 40 428
Fragments arranged by size:
1135 428 173 40
DraIII CAC_nnn'GTG
Cuts at: 0 656 1415 1776
Size: 656 759 361
Fragments arranged by size:
759 656 361
EaeI y'GGCC_r
Cuts at: 0 2 167 287 425 1118 1292 1400 1430
Size: 2 165 120 138 693 174 108 30
Cuts at: 1430 1520 1550 1658 1751 1776
Size: 90 30 108 93 25
Fragments arranged by size:
693 174 165 138 120 108 108 93
90 30 30 25 2
EarI CTCTTCn'nnn_
Cuts at: 0 145 187 612 763 868 1690 1776
Size: 145 42 425 151 105 822 86
Fragments arranged by size:
822 425 151 145 105 86 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 672 1776
Size: 672 1104
EcoICRI GAG'CTC
Cuts at: 0 534 1776
Size: 534 1242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1222 1352 1671 1776
Size: 520 702 130 319 105
Fragments arranged by size:
702 520 319 130 105
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1294 1776
Size: 1294 482
EcoO109I rG'GnC_Cy
Cuts at: 0 18 156 585 967 1179 1206 1587 1776
Size: 18 138 429 382 212 27 381 189
Fragments arranged by size:
429 382 381 212 189 138 27 18
HaeII r_GCGC'y
Cuts at: 0 139 1776
Size: 139 1637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 609 641 1610 1631 1642 1663 1776
Size: 609 32 969 21 11 21 113
Fragments arranged by size:
969 609 113 32 21 21 11
HindIII A'AGCT_T
Cuts at: 0 568 1776
Size: 568 1208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1580 1745 1776
Size: 281 1299 165 31
Fragments arranged by size:
1299 281 165 31
MscI TGG'CCA
Cuts at: 0 4 289 1120 1294 1402 1432 1522 1552
Size: 4 285 831 174 108 30 90 30
Cuts at: 1552 1660 1753 1776
Size: 108 93 23
Fragments arranged by size:
831 285 174 108 108 93 90 30
30 23 4
MslI CAynn'nnrTG
Cuts at: 0 130 295 442 1015 1138 1776
Size: 130 165 147 573 123 638
Fragments arranged by size:
638 573 165 147 130 123
MspA1I CmG'CkG
Cuts at: 0 474 1670 1776
Size: 474 1196 106
Fragments arranged by size:
1196 474 106
NcoI C'CATG_G
Cuts at: 0 818 1466 1776
Size: 818 648 310
Fragments arranged by size:
818 648 310
NspI r_CATG'y
Cuts at: 0 460 1776
Size: 460 1316
PasI CC'CwG_GG
Cuts at: 0 1165 1192 1776
Size: 1165 27 584
Fragments arranged by size:
1165 584 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 11 1776
Size: 11 1765
PfoI T'CCnGG_A
Cuts at: 0 464 536 1776
Size: 464 72 1240
Fragments arranged by size:
1240 464 72
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 775 807 1776
Size: 775 32 969
Fragments arranged by size:
969 775 32
PpuMI rG'GwC_Cy
Cuts at: 0 1179 1587 1776
Size: 1179 408 189
Fragments arranged by size:
1179 408 189
PspOMI G'GGCC_C
Cuts at: 0 966 1776
Size: 966 810
PstI C_TGCA'G
Cuts at: 0 857 1355 1776
Size: 857 498 421
Fragments arranged by size:
857 498 421
PvuI CG_AT'CG
Cuts at: 0 1312 1776
Size: 1312 464
PvuII CAG'CTG
Cuts at: 0 474 1670 1776
Size: 474 1196 106
Fragments arranged by size:
1196 474 106
SacI G_AGCT'C
Cuts at: 0 536 1776
Size: 536 1240
SapI GCTCTTCn'nnn_
Cuts at: 0 145 763 1776
Size: 145 618 1013
Fragments arranged by size:
1013 618 145
SexAI A'CCwGG_T
Cuts at: 0 728 1776
Size: 728 1048
SfcI C'TryA_G
Cuts at: 0 853 1351 1776
Size: 853 498 425
Fragments arranged by size:
853 498 425
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 25 1776
Size: 25 1751
SmaI CCC'GGG
Cuts at: 0 1036 1612 1776
Size: 1036 576 164
Fragments arranged by size:
1036 576 164
SmlI C'TyrA_G
Cuts at: 0 247 1776
Size: 247 1529
SrfI GCCC'GGGC
Cuts at: 0 1612 1776
Size: 1612 164
StuI AGG'CCT
Cuts at: 0 106 1776
Size: 106 1670
StyI C'CwwG_G
Cuts at: 0 818 1085 1295 1466 1776
Size: 818 267 210 171 310
Fragments arranged by size:
818 310 267 210 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 667 738 964 1264 1776
Size: 667 71 226 300 512
Fragments arranged by size:
667 512 300 226 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 446 478 1776
Size: 446 32 1298
Fragments arranged by size:
1298 446 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 129 1776
Size: 129 1647
XmaI C'CCGG_G
Cuts at: 0 1034 1610 1776
Size: 1034 576 166
Fragments arranged by size:
1034 576 166
Enzymes that do cut:
AarI AcuI AflII AlfI AloI AlwNI ApaI ApaLI
AvaI BanI BanII BbsI BbvCI BcgI BclI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI
NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuI PvuII SacI SapI SexAI SfcI SfiI SmaI
SmlI SrfI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BfrBI BglII BlpI BmtI BplI BpuEI
BsaI BsaAI BsaHI BsiWI BsmI BsmBI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI
RsrII SacII SalI SanDI SbfI ScaI SfoI SgfI
SgrAI SnaBI SpeI SphI SspI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI