Restriction map for
pFN21AE6489
(Linear) (Six-base) MAPSORT of: KIEE6489.seq Check: 1872 from: 1 to: 1779
KIEE6489.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1456 1779
Size: 1456 323
AccI GT'mk_AC
Cuts at: 0 1046 1779
Size: 1046 733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 1779
Size: 626 1153
AflIII A'CryG_T
Cuts at: 0 729 1589 1779
Size: 729 860 190
Fragments arranged by size:
860 729 190
AleI CACnn'nnGTG
Cuts at: 0 178 1380 1779
Size: 178 1202 399
Fragments arranged by size:
1202 399 178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 569 1779
Size: 569 1210
AlwNI CAG_nnn'CTG
Cuts at: 0 397 1779
Size: 397 1382
ApoI r'AATT_y
Cuts at: 0 156 197 353 439 577 1779
Size: 156 41 156 86 138 1202
Fragments arranged by size:
1202 156 156 138 86 41
AseI AT'TA_AT
Cuts at: 0 871 1779
Size: 871 908
AvaI C'yCGr_G
Cuts at: 0 752 1779
Size: 752 1027
BanI G'GyrC_C
Cuts at: 0 998 1779
Size: 998 781
BbsI GAAGACnn'nnnn_
Cuts at: 0 194 433 1779
Size: 194 239 1346
Fragments arranged by size:
1346 239 194
BbvCI CC'TCA_GC
Cuts at: 0 895 1779
Size: 895 884
BfrBI ATG'CAT
Cuts at: 0 501 505 1779
Size: 501 4 1274
Fragments arranged by size:
1274 501 4
BmgBI CAC'GTC
Cuts at: 0 1378 1779
Size: 1378 401
BmrI ACTGGGnnnn_n'
Cuts at: 0 85 1779
Size: 85 1694
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1360 1779
Size: 1360 419
Bpu10I CC'TnA_GC
Cuts at: 0 474 895 1779
Size: 474 421 884
Fragments arranged by size:
884 474 421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 603 1779
Size: 603 1176
BsaI GGTCTCn'nnnn_
Cuts at: 0 406 1154 1779
Size: 406 748 625
Fragments arranged by size:
748 625 406
BsaHI Gr'CG_yC
Cuts at: 0 7 1672 1779
Size: 7 1665 107
Fragments arranged by size:
1665 107 7
BsaWI w'CCGG_w
Cuts at: 0 884 959 1163 1779
Size: 884 75 204 616
Fragments arranged by size:
884 616 204 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1172 1202 1221 1251 1299 1329 1454 1484
Size: 1172 30 19 30 48 30 125 30
Cuts at: 1484 1779
Size: 295
Fragments arranged by size:
1172 295 125 48 30 30 30 30
19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1323 1779
Size: 1323 456
BseYI C'CCAG_C
Cuts at: 0 390 452 1779
Size: 390 62 1327
Fragments arranged by size:
1327 390 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1050 1614 1637 1779
Size: 627 423 564 23 142
Fragments arranged by size:
627 564 423 142 23
BsiHKAI G_wGCw'C
Cuts at: 0 1450 1735 1779
Size: 1450 285 44
Fragments arranged by size:
1450 285 44
BsmI GAATG_Cn'
Cuts at: 0 976 1405 1779
Size: 976 429 374
Fragments arranged by size:
976 429 374
Bsp1286I G_dGCh'C
Cuts at: 0 1450 1735 1779
Size: 1450 285 44
Fragments arranged by size:
1450 285 44
BspEI T'CCGG_A
Cuts at: 0 884 959 1779
Size: 884 75 820
Fragments arranged by size:
884 820 75
BspHI T'CATG_A
Cuts at: 0 134 1509 1779
Size: 134 1375 270
Fragments arranged by size:
1375 270 134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1456 1779
Size: 1456 323
BsrDI GCAATG_nn'
Cuts at: 0 839 1779
Size: 839 940
BsrGI T'GTAC_A
Cuts at: 0 375 1779
Size: 375 1404
BstAPI GCAn_nnn'nTGC
Cuts at: 0 809 1779
Size: 809 970
BstEII G'GTnAC_C
Cuts at: 0 737 1779
Size: 737 1042
BstXI CCAn_nnnn'nTGG
Cuts at: 0 924 1779
Size: 924 855
Bsu36I CC'TnA_GG
Cuts at: 0 319 1779
Size: 319 1460
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 182 1779
Size: 182 1597
BtsI GCAGTG_nn'
Cuts at: 0 84 344 810 1320 1779
Size: 84 260 466 510 459
Fragments arranged by size:
510 466 459 260 84
DraI TTT'AAA
Cuts at: 0 984 1779
Size: 984 795
DrdI GACnn_nn'nnGTC
Cuts at: 0 1716 1779
Size: 1716 63
EaeI y'GGCC_r
Cuts at: 0 221 1779
Size: 221 1558
EarI CTCTTCn'nnn_
Cuts at: 0 641 773 871 1779
Size: 641 132 98 908
Fragments arranged by size:
908 641 132 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1470 1779
Size: 1470 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 1779
Size: 626 1153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 87 119 1357 1389 1779
Size: 87 32 1238 32 390
Fragments arranged by size:
1238 390 87 32 32
HincII GTy'rAC
Cuts at: 0 468 1779
Size: 468 1311
HindIII A'AGCT_T
Cuts at: 0 122 1779
Size: 122 1657
MfeI C'AATT_G
Cuts at: 0 176 1779
Size: 176 1603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1314 1623 1779
Size: 1314 309 156
Fragments arranged by size:
1314 309 156
MslI CAynn'nnrTG
Cuts at: 0 178 1380 1779
Size: 178 1202 399
Fragments arranged by size:
1202 399 178
MspA1I CmG'CkG
Cuts at: 0 15 397 1779
Size: 15 382 1382
Fragments arranged by size:
1382 382 15
NdeI CA'TA_TG
Cuts at: 0 113 1779
Size: 113 1666
NsiI A_TGCA'T
Cuts at: 0 503 507 1779
Size: 503 4 1272
Fragments arranged by size:
1272 503 4
NspI r_CATG'y
Cuts at: 0 29 505 509 733 1593 1779
Size: 29 476 4 224 860 186
Fragments arranged by size:
860 476 224 186 29 4
PciI A'CATG_T
Cuts at: 0 729 1589 1779
Size: 729 860 190
Fragments arranged by size:
860 729 190
PflMI CCAn_nnn'nTGG
Cuts at: 0 397 1779
Size: 397 1382
PfoI T'CCnGG_A
Cuts at: 0 1021 1779
Size: 1021 758
PsiI TTA'TAA
Cuts at: 0 1688 1779
Size: 1688 91
PstI C_TGCA'G
Cuts at: 0 351 1779
Size: 351 1428
PvuII CAG'CTG
Cuts at: 0 397 1779
Size: 397 1382
SfcI C'TryA_G
Cuts at: 0 228 347 1106 1352 1499 1779
Size: 228 119 759 246 147 280
Fragments arranged by size:
759 280 246 228 147 119
SmlI C'TyrA_G
Cuts at: 0 618 1779
Size: 618 1161
SphI G_CATG'C
Cuts at: 0 29 505 1779
Size: 29 476 1274
Fragments arranged by size:
1274 476 29
SspI AAT'ATT
Cuts at: 0 760 1684 1779
Size: 760 924 95
Fragments arranged by size:
924 760 95
StyI C'CwwG_G
Cuts at: 0 106 1779
Size: 106 1673
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 666 918 1315 1779
Size: 666 252 397 464
Fragments arranged by size:
666 464 397 252
TatI w'GTAC_w
Cuts at: 0 375 564 1231 1779
Size: 375 189 667 548
Fragments arranged by size:
667 548 375 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1172 1204 1299 1331 1779
Size: 1172 32 95 32 448
Fragments arranged by size:
1172 448 95 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 924 1779
Size: 924 855
XmnI GAAnn'nnTTC
Cuts at: 0 124 578 1779
Size: 124 454 1201
Fragments arranged by size:
1201 454 124
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApoI
AseI AvaI BanI BbsI BbvCI BfrBI BmgBI BmrI
BplI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrGI BstAPI BstEII BstXI Bsu36I BtgZI BtsI
DraI DrdI EaeI EarI EciI Eco57MI Hin4I HincII
HindIII MfeI MmeI MslI MspA1I NdeI NsiI NspI
PciI PflMI PfoI PsiI PstI PvuII SfcI SmlI
SphI SspI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmtI BpmI BsaAI BsaBI BsiEI BsiWI BsmBI BsrBI
BsrFI BssHII BssSI BstBI BstYI BstZ17I BtgI ClaI
CspCI DraIII EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I
XbaI XhoI XmaI ZraI