Restriction map for
pFN21AE6487
(Linear) (Six-base) MAPSORT of: KIEE6487.seq Check: 208 from: 1 to: 1521
KIEE6487.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 355 865 1521
Size: 184 171 510 656
Fragments arranged by size:
656 510 184 171
AflIII A'CryG_T
Cuts at: 0 1025 1521
Size: 1025 496
AleI CACnn'nnGTG
Cuts at: 0 466 1521
Size: 466 1055
AlwNI CAG_nnn'CTG
Cuts at: 0 1311 1521
Size: 1311 210
ApaI G_GGCC'C
Cuts at: 0 126 1521
Size: 126 1395
ApaLI G'TGCA_C
Cuts at: 0 1034 1521
Size: 1034 487
ApoI r'AATT_y
Cuts at: 0 1066 1521
Size: 1066 455
AvaI C'yCGr_G
Cuts at: 0 515 637 1124 1521
Size: 515 122 487 397
Fragments arranged by size:
515 487 397 122
BamHI G'GATC_C
Cuts at: 0 1314 1521
Size: 1314 207
BanI G'GyrC_C
Cuts at: 0 315 1301 1521
Size: 315 986 220
Fragments arranged by size:
986 315 220
BanII G_rGCy'C
Cuts at: 0 117 126 476 826 1521
Size: 117 9 350 350 695
Fragments arranged by size:
695 350 350 117 9
BbvCI CC'TCA_GC
Cuts at: 0 76 775 1521
Size: 76 699 746
Fragments arranged by size:
746 699 76
Bme1580I G_kGCm'C
Cuts at: 0 126 215 1038 1521
Size: 126 89 823 483
Fragments arranged by size:
823 483 126 89
BmgBI CAC'GTC
Cuts at: 0 1100 1521
Size: 1100 421
BmrI ACTGGGnnnn_n'
Cuts at: 0 790 1521
Size: 790 731
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1150 1521
Size: 1150 371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 367 1039 1521
Size: 178 189 672 482
Fragments arranged by size:
672 482 189 178
Bpu10I CC'TnA_GC
Cuts at: 0 76 775 1521
Size: 76 699 746
Fragments arranged by size:
746 699 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 1521
Size: 927 594
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 172 680 1521
Size: 7 165 508 841
Fragments arranged by size:
841 508 165 7
BsaHI Gr'CG_yC
Cuts at: 0 760 1043 1521
Size: 760 283 478
Fragments arranged by size:
760 478 283
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 535 565 1180 1210 1521
Size: 535 30 615 30 311
Fragments arranged by size:
615 535 311 30 30
BseYI C'CCAG_C
Cuts at: 0 57 1350 1521
Size: 57 1293 171
Fragments arranged by size:
1293 171 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1006 1080 1434 1521
Size: 475 531 74 354 87
Fragments arranged by size:
531 475 354 87 74
BsiHKAI G_wGCw'C
Cuts at: 0 117 630 1038 1229 1521
Size: 117 513 408 191 292
Fragments arranged by size:
513 408 292 191 117
Bsp1286I G_dGCh'C
Cuts at: 0 117 126 215 476 630 826 1038 1229
Size: 117 9 89 261 154 196 212 191
Cuts at: 1229 1521
Size: 292
Fragments arranged by size:
292 261 212 196 191 154 117 89
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1028 1241 1367 1521
Size: 1028 213 126 154
Fragments arranged by size:
1028 213 154 126
BsrDI GCAATG_nn'
Cuts at: 0 656 668 809 1521
Size: 656 12 141 712
Fragments arranged by size:
712 656 141 12
BsrFI r'CCGG_y
Cuts at: 0 247 1521
Size: 247 1274
BsrGI T'GTAC_A
Cuts at: 0 1408 1521
Size: 1408 113
BstAPI GCAn_nnn'nTGC
Cuts at: 0 589 1521
Size: 589 932
BstEII G'GTnAC_C
Cuts at: 0 204 1521
Size: 204 1317
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 468 1521
Size: 156 312 1053
Fragments arranged by size:
1053 312 156
BstYI r'GATC_y
Cuts at: 0 839 1314 1521
Size: 839 475 207
Fragments arranged by size:
839 475 207
BtgI C'CryG_G
Cuts at: 0 875 1376 1521
Size: 875 501 145
Fragments arranged by size:
875 501 145
BtsI GCAGTG_nn'
Cuts at: 0 567 569 1326 1385 1521
Size: 567 2 757 59 136
Fragments arranged by size:
757 567 136 59 2
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 651 686 1358 1393 1521
Size: 651 35 672 35 128
Fragments arranged by size:
672 651 128 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 186 1521
Size: 186 1335
EaeI y'GGCC_r
Cuts at: 0 872 989 1521
Size: 872 117 532
Fragments arranged by size:
872 532 117
EcoICRI GAG'CTC
Cuts at: 0 115 1521
Size: 115 1406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 184 355 367 865 1039 1521
Size: 178 6 171 12 498 174 482
Fragments arranged by size:
498 482 178 174 171 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 448 1186 1249 1521
Size: 448 738 63 272
Fragments arranged by size:
738 448 272 63
EcoO109I rG'GnC_Cy
Cuts at: 0 122 123 804 1308 1356 1428 1521
Size: 122 1 681 504 48 72 93
Fragments arranged by size:
681 504 122 93 72 48 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 612 644 677 709 887 919 1180 1212
Size: 612 32 33 32 178 32 261 32
Cuts at: 1212 1415 1447 1521
Size: 203 32 74
Fragments arranged by size:
612 261 203 178 74 33 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 645 1521
Size: 645 876
MscI TGG'CCA
Cuts at: 0 874 991 1521
Size: 874 117 530
Fragments arranged by size:
874 530 117
MslI CAynn'nnrTG
Cuts at: 0 451 466 1521
Size: 451 15 1055
Fragments arranged by size:
1055 451 15
MspA1I CmG'CkG
Cuts at: 0 24 38 379 765 1521
Size: 24 14 341 386 756
Fragments arranged by size:
756 386 341 24 14
NaeI GCC'GGC
Cuts at: 0 249 1521
Size: 249 1272
NcoI C'CATG_G
Cuts at: 0 875 1521
Size: 875 646
NdeI CA'TA_TG
Cuts at: 0 964 1521
Size: 964 557
NgoMIV G'CCGG_C
Cuts at: 0 247 1521
Size: 247 1274
NspI r_CATG'y
Cuts at: 0 255 1029 1521
Size: 255 774 492
Fragments arranged by size:
774 492 255
PasI CC'CwG_GG
Cuts at: 0 594 1521
Size: 594 927
PciI A'CATG_T
Cuts at: 0 1025 1521
Size: 1025 496
PflMI CCAn_nnn'nTGG
Cuts at: 0 1311 1521
Size: 1311 210
PpuMI rG'GwC_Cy
Cuts at: 0 804 1356 1521
Size: 804 552 165
Fragments arranged by size:
804 552 165
PspOMI G'GGCC_C
Cuts at: 0 122 1521
Size: 122 1399
PspXI vC'TCGA_Gb
Cuts at: 0 1124 1521
Size: 1124 397
PvuII CAG'CTG
Cuts at: 0 38 379 1521
Size: 38 341 1142
Fragments arranged by size:
1142 341 38
SacI G_AGCT'C
Cuts at: 0 117 1521
Size: 117 1404
SexAI A'CCwGG_T
Cuts at: 0 1181 1521
Size: 1181 340
SfcI C'TryA_G
Cuts at: 0 95 732 1521
Size: 95 637 789
Fragments arranged by size:
789 637 95
SmlI C'TyrA_G
Cuts at: 0 515 906 1124 1521
Size: 515 391 218 397
Fragments arranged by size:
515 397 391 218
SphI G_CATG'C
Cuts at: 0 255 1521
Size: 255 1266
StuI AGG'CCT
Cuts at: 0 197 230 1244 1521
Size: 197 33 1014 277
Fragments arranged by size:
1014 277 197 33
StyI C'CwwG_G
Cuts at: 0 536 875 1521
Size: 536 339 646
Fragments arranged by size:
646 536 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 843 1521
Size: 843 678
TatI w'GTAC_w
Cuts at: 0 932 1408 1521
Size: 932 476 113
Fragments arranged by size:
932 476 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 535 567 1028 1060 1521
Size: 535 32 461 32 461
Fragments arranged by size:
535 461 461 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 711 1521
Size: 711 810
XhoI C'TCGA_G
Cuts at: 0 515 1124 1521
Size: 515 609 397
Fragments arranged by size:
609 515 397
XmnI GAAnn'nnTTC
Cuts at: 0 1066 1521
Size: 1066 455
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbvCI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI BtgI BtsI CspCI DrdI EaeI EcoICRI
Eco57MI EcoNI EcoO109I Hin4I HincII MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PpuMI PspOMI PspXI PvuII SacI SexAI SfcI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmtI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI EarI
EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PpiI PshAI PsiI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XmaI ZraI