Restriction map for
pFN21AE6485
(Linear) (Six-base) MAPSORT of: KIEE6485.seq Check: 4481 from: 1 to: 1866
KIEE6485.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AccI GT'mk_AC
Cuts at: 0 1034 1866
Size: 1034 832
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1235 1254 1866
Size: 1235 19 612
Fragments arranged by size:
1235 612 19
AlwNI CAG_nnn'CTG
Cuts at: 0 1255 1749 1866
Size: 1255 494 117
Fragments arranged by size:
1255 494 117
ApaLI G'TGCA_C
Cuts at: 0 83 1866
Size: 83 1783
ApoI r'AATT_y
Cuts at: 0 179 345 1866
Size: 179 166 1521
Fragments arranged by size:
1521 179 166
BanII G_rGCy'C
Cuts at: 0 22 875 1122 1866
Size: 22 853 247 744
Fragments arranged by size:
853 744 247 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 958 1723 1866
Size: 958 765 143
Fragments arranged by size:
958 765 143
BciVI GTATCCnnnnn_n'
Cuts at: 0 913 1866
Size: 913 953
BclI T'GATC_A
Cuts at: 0 1356 1866
Size: 1356 510
Bme1580I G_kGCm'C
Cuts at: 0 87 1866
Size: 87 1779
BmrI ACTGGGnnnn_n'
Cuts at: 0 372 675 1866
Size: 372 303 1191
Fragments arranged by size:
1191 372 303
BmtI G_CTAG'C
Cuts at: 0 1579 1866
Size: 1579 287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 391 1866
Size: 41 350 1475
Fragments arranged by size:
1475 350 41
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 344 1866
Size: 344 1522
BseYI C'CCAG_C
Cuts at: 0 15 1836 1866
Size: 15 1821 30
Fragments arranged by size:
1821 30 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 437 1866
Size: 48 389 1429
Fragments arranged by size:
1429 389 48
BsiHKAI G_wGCw'C
Cuts at: 0 87 875 1866
Size: 87 788 991
Fragments arranged by size:
991 788 87
Bsp1286I G_dGCh'C
Cuts at: 0 22 87 875 1122 1866
Size: 22 65 788 247 744
Fragments arranged by size:
788 744 247 65 22
BsrGI T'GTAC_A
Cuts at: 0 1282 1866
Size: 1282 584
BstXI CCAn_nnnn'nTGG
Cuts at: 0 665 1866
Size: 665 1201
BstYI r'GATC_y
Cuts at: 0 285 1462 1866
Size: 285 1177 404
Fragments arranged by size:
1177 404 285
BstZ17I GTA'TAC
Cuts at: 0 1035 1866
Size: 1035 831
Bsu36I CC'TnA_GG
Cuts at: 0 1711 1866
Size: 1711 155
BtgI C'CryG_G
Cuts at: 0 42 1436 1823 1866
Size: 42 1394 387 43
Fragments arranged by size:
1394 387 43 42
BtsI GCAGTG_nn'
Cuts at: 0 31 1320 1362 1866
Size: 31 1289 42 504
Fragments arranged by size:
1289 504 42 31
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 414 449 1866
Size: 414 35 1417
Fragments arranged by size:
1417 414 35
DraI TTT'AAA
Cuts at: 0 344 608 956 1866
Size: 344 264 348 910
Fragments arranged by size:
910 348 344 264
EarI CTCTTCn'nnn_
Cuts at: 0 128 362 880 1866
Size: 128 234 518 986
Fragments arranged by size:
986 518 234 128
EcoICRI GAG'CTC
Cuts at: 0 873 1866
Size: 873 993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 391 1235 1254 1866
Size: 41 350 844 19 612
Fragments arranged by size:
844 612 350 41 19
EcoRV GAT'ATC
Cuts at: 0 732 1866
Size: 732 1134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 982 1866
Size: 982 884
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 206 238 1454 1486 1795 1827
Size: 62 32 112 32 1216 32 309 32
Cuts at: 1827 1866
Size: 39
Fragments arranged by size:
1216 309 112 62 39 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1601 1866
Size: 1601 265
HindIII A'AGCT_T
Cuts at: 0 703 1866
Size: 703 1163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 1866
Size: 396 1470
MslI CAynn'nnrTG
Cuts at: 0 41 1325 1866
Size: 41 1284 541
Fragments arranged by size:
1284 541 41
NcoI C'CATG_G
Cuts at: 0 42 1436 1823 1866
Size: 42 1394 387 43
Fragments arranged by size:
1394 387 43 42
NdeI CA'TA_TG
Cuts at: 0 800 1691 1866
Size: 800 891 175
Fragments arranged by size:
891 800 175
NheI G'CTAG_C
Cuts at: 0 1575 1866
Size: 1575 291
NspI r_CATG'y
Cuts at: 0 486 1866
Size: 486 1380
PflMI CCAn_nnn'nTGG
Cuts at: 0 64 1866
Size: 64 1802
PfoI T'CCnGG_A
Cuts at: 0 57 1866
Size: 57 1809
PsiI TTA'TAA
Cuts at: 0 1297 1866
Size: 1297 569
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1018 1050 1266 1298 1866
Size: 1018 32 216 32 568
Fragments arranged by size:
1018 568 216 32 32
PstI C_TGCA'G
Cuts at: 0 863 1676 1866
Size: 863 813 190
Fragments arranged by size:
863 813 190
SacI G_AGCT'C
Cuts at: 0 875 1866
Size: 875 991
SapI GCTCTTCn'nnn_
Cuts at: 0 128 880 1866
Size: 128 752 986
Fragments arranged by size:
986 752 128
SfcI C'TryA_G
Cuts at: 0 859 1672 1866
Size: 859 813 194
Fragments arranged by size:
859 813 194
StyI C'CwwG_G
Cuts at: 0 42 1003 1436 1552 1823 1866
Size: 42 961 433 116 271 43
Fragments arranged by size:
961 433 271 116 43 42
SwaI ATTT'AAAT
Cuts at: 0 344 1866
Size: 344 1522
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 1850 1866
Size: 322 1528 16
Fragments arranged by size:
1528 322 16
TatI w'GTAC_w
Cuts at: 0 1282 1817 1866
Size: 1282 535 49
Fragments arranged by size:
1282 535 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 506 538 1866
Size: 506 32 1328
Fragments arranged by size:
1328 506 32
Tth111I GACn'n_nGTC
Cuts at: 0 157 1704 1866
Size: 157 1547 162
Fragments arranged by size:
1547 162 157
XmnI GAAnn'nnTTC
Cuts at: 0 122 1866
Size: 122 1744
Enzymes that do cut:
AccI AcuI AlwNI ApaLI ApoI BanII BbsI BciVI
BclI Bme1580I BmrI BmtI BpmI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrGI BstXI BstYI BstZ17I Bsu36I BtgI
BtsI CspCI DraI EarI EcoICRI Eco57MI EcoRV FalI
Hin4I HincII HindIII MmeI MslI NcoI NdeI NheI
NspI PflMI PfoI PsiI PsrI PstI SacI SapI
SfcI StyI SwaI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BfrBI BglI BglII BlpI BmgBI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BtgZI ClaI DraIII
DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NgoMIV NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI XbaI
XcmI XhoI XmaI ZraI