Restriction map for
pFN21AE6479
(Linear) (Six-base) MAPSORT of: KIEE6479.seq Check: 8088 from: 1 to: 1425
KIEE6479.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
Acc65I G'GTAC_C
Cuts at: 0 929 1425
Size: 929 496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 1149 1425
Size: 1070 79 276
Fragments arranged by size:
1070 276 79
AleI CACnn'nnGTG
Cuts at: 0 352 1425
Size: 352 1073
AlwNI CAG_nnn'CTG
Cuts at: 0 508 1425
Size: 508 917
ApaI G_GGCC'C
Cuts at: 0 406 1425
Size: 406 1019
BanI G'GyrC_C
Cuts at: 0 293 929 961 1425
Size: 293 636 32 464
Fragments arranged by size:
636 464 293 32
BanII G_rGCy'C
Cuts at: 0 172 259 344 406 703 745 1332 1425
Size: 172 87 85 62 297 42 587 93
Fragments arranged by size:
587 297 172 93 87 85 62 42
BbeI G_GCGC'C
Cuts at: 0 965 1425
Size: 965 460
BbsI GAAGACnn'nnnn_
Cuts at: 0 280 1210 1425
Size: 280 930 215
Fragments arranged by size:
930 280 215
BglI GCCn_nnn'nGGC
Cuts at: 0 488 632 1425
Size: 488 144 793
Fragments arranged by size:
793 488 144
Bme1580I G_kGCm'C
Cuts at: 0 296 406 1425
Size: 296 110 1019
Fragments arranged by size:
1019 296 110
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 522 1425
Size: 522 903
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1425
Size: 340 1085
BsaI GGTCTCn'nnnn_
Cuts at: 0 1161 1413 1425
Size: 1161 252 12
Fragments arranged by size:
1161 252 12
BsaBI GATnn'nnATC
Cuts at: 0 38 1425
Size: 38 1387
BsaHI Gr'CG_yC
Cuts at: 0 962 1425
Size: 962 463
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 188 218 1425
Size: 188 30 1207
Fragments arranged by size:
1207 188 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 829 868 1425
Size: 511 318 39 557
Fragments arranged by size:
557 511 318 39
BseYI C'CCAG_C
Cuts at: 0 252 329 594 1186 1412 1425
Size: 252 77 265 592 226 13
Fragments arranged by size:
592 265 252 226 77 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 684 874 1425
Size: 325 359 190 551
Fragments arranged by size:
551 359 325 190
BsiHKAI G_wGCw'C
Cuts at: 0 821 1425
Size: 821 604
BsmBI CGTCTCn'nnnn_
Cuts at: 0 356 941 1425
Size: 356 585 484
Fragments arranged by size:
585 484 356
Bsp1286I G_dGCh'C
Cuts at: 0 172 259 296 344 406 703 745 821
Size: 172 87 37 48 62 297 42 76
Cuts at: 821 1332 1425
Size: 511 93
Fragments arranged by size:
511 297 172 93 87 76 62 48
42 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1056 1425
Size: 1056 369
BsrBI CCG'CTC
Cuts at: 0 228 1425
Size: 228 1197
BsrFI r'CCGG_y
Cuts at: 0 412 1425
Size: 412 1013
BsrGI T'GTAC_A
Cuts at: 0 1127 1425
Size: 1127 298
BssSI C'ACGA_G
Cuts at: 0 1030 1425
Size: 1030 395
BstXI CCAn_nnnn'nTGG
Cuts at: 0 870 1005 1386 1425
Size: 870 135 381 39
Fragments arranged by size:
870 381 135 39
BstYI r'GATC_y
Cuts at: 0 221 881 1425
Size: 221 660 544
Fragments arranged by size:
660 544 221
Bsu36I CC'TnA_GG
Cuts at: 0 628 1425
Size: 628 797
BtgI C'CryG_G
Cuts at: 0 794 948 1137 1280 1425
Size: 794 154 189 143 145
Fragments arranged by size:
794 189 154 145 143
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 56 91 1425
Size: 56 35 1334
Fragments arranged by size:
1334 56 35
EaeI y'GGCC_r
Cuts at: 0 100 410 797 1388 1425
Size: 100 310 387 591 37
Fragments arranged by size:
591 387 310 100 37
EarI CTCTTCn'nnn_
Cuts at: 0 103 806 1188 1425
Size: 103 703 382 237
Fragments arranged by size:
703 382 237 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1070 1149 1425
Size: 340 730 79 276
Fragments arranged by size:
730 340 276 79
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1402 1425
Size: 1402 23
EcoO109I rG'GnC_Cy
Cuts at: 0 402 513 1425
Size: 402 111 912
Fragments arranged by size:
912 402 111
EcoRV GAT'ATC
Cuts at: 0 189 1425
Size: 189 1236
HaeII r_GCGC'y
Cuts at: 0 965 1425
Size: 965 460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 104 136 467 499 1425
Size: 104 32 331 32 926
Fragments arranged by size:
926 331 104 32 32
HincII GTy'rAC
Cuts at: 0 468 1425
Size: 468 957
KasI G'GCGC_C
Cuts at: 0 961 1425
Size: 961 464
KpnI G_GTAC'C
Cuts at: 0 933 1425
Size: 933 492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1184 1425
Size: 284 900 241
Fragments arranged by size:
900 284 241
MslI CAynn'nnrTG
Cuts at: 0 299 352 655 913 1425
Size: 299 53 303 258 512
Fragments arranged by size:
512 303 299 258 53
MspA1I CmG'CkG
Cuts at: 0 1318 1416 1425
Size: 1318 98 9
Fragments arranged by size:
1318 98 9
NaeI GCC'GGC
Cuts at: 0 414 1425
Size: 414 1011
NarI GG'CG_CC
Cuts at: 0 962 1425
Size: 962 463
NcoI C'CATG_G
Cuts at: 0 948 1425
Size: 948 477
NgoMIV G'CCGG_C
Cuts at: 0 412 1425
Size: 412 1013
PasI CC'CwG_GG
Cuts at: 0 1151 1425
Size: 1151 274
PshAI GACnn'nnGTC
Cuts at: 0 1166 1425
Size: 1166 259
PspOMI G'GGCC_C
Cuts at: 0 402 1425
Size: 402 1023
PstI C_TGCA'G
Cuts at: 0 150 315 1425
Size: 150 165 1110
Fragments arranged by size:
1110 165 150
PvuII CAG'CTG
Cuts at: 0 1416 1425
Size: 1416 9
SapI GCTCTTCn'nnn_
Cuts at: 0 1188 1425
Size: 1188 237
SfcI C'TryA_G
Cuts at: 0 146 311 1425
Size: 146 165 1114
Fragments arranged by size:
1114 165 146
SfoI GGC'GCC
Cuts at: 0 963 1425
Size: 963 462
StyI C'CwwG_G
Cuts at: 0 863 948 1425
Size: 863 85 477
Fragments arranged by size:
863 477 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 63 1425
Size: 63 1362
TatI w'GTAC_w
Cuts at: 0 26 1127 1425
Size: 26 1101 298
Fragments arranged by size:
1101 298 26
Tth111I GACn'n_nGTC
Cuts at: 0 568 1425
Size: 568 857
Enzymes that do cut:
Acc65I AcuI AleI AlwNI ApaI BanI BanII BbeI
BbsI BglI Bme1580I BplI BpmI BsaI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI
CspCI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV HaeII
Hin4I HincII KasI KpnI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PshAI PspOMI PstI PvuII
SapI SfcI SfoI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI
BsaAI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrDI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoRI FalI
FseI FspI FspAI HindIII HpaI MfeI MluI MscI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XcmI XhoI XmaI XmnI ZraI