Restriction map for
pFN21AE6477
(Linear) (Six-base) MAPSORT of: KIEE6477.seq Check: 2701 from: 1 to: 1515
KIEE6477.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AatII G_ACGT'C
Cuts at: 0 1412 1515
Size: 1412 103
AccI GT'mk_AC
Cuts at: 0 1009 1442 1515
Size: 1009 433 73
Fragments arranged by size:
1009 433 73
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 1515
Size: 1285 230
AflIII A'CryG_T
Cuts at: 0 110 1515
Size: 110 1405
AleI CACnn'nnGTG
Cuts at: 0 565 731 983 1515
Size: 565 166 252 532
Fragments arranged by size:
565 532 252 166
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 43 75 1515
Size: 43 32 1440
Fragments arranged by size:
1440 43 32
AlwNI CAG_nnn'CTG
Cuts at: 0 782 1515
Size: 782 733
ApaI G_GGCC'C
Cuts at: 0 228 1515
Size: 228 1287
ApoI r'AATT_y
Cuts at: 0 552 1515
Size: 552 963
BamHI G'GATC_C
Cuts at: 0 878 1515
Size: 878 637
BanI G'GyrC_C
Cuts at: 0 1312 1515
Size: 1312 203
BanII G_rGCy'C
Cuts at: 0 228 428 586 1058 1358 1515
Size: 228 200 158 472 300 157
Fragments arranged by size:
472 300 228 200 158 157
BbsI GAAGACnn'nnnn_
Cuts at: 0 1377 1515
Size: 1377 138
BbvCI CC'TCA_GC
Cuts at: 0 722 1515
Size: 722 793
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 495 529 829 863 1515
Size: 495 34 300 34 652
Fragments arranged by size:
652 495 300 34 34
BclI T'GATC_A
Cuts at: 0 1181 1515
Size: 1181 334
BglI GCCn_nnn'nGGC
Cuts at: 0 973 994 1268 1515
Size: 973 21 274 247
Fragments arranged by size:
973 274 247 21
BglII A'GATC_T
Cuts at: 0 1451 1515
Size: 1451 64
Bme1580I G_kGCm'C
Cuts at: 0 228 1515
Size: 228 1287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 1515
Size: 30 1485
Bpu10I CC'TnA_GC
Cuts at: 0 722 1474 1515
Size: 722 752 41
Fragments arranged by size:
752 722 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1515
Size: 826 689
BsaBI GATnn'nnATC
Cuts at: 0 1180 1515
Size: 1180 335
BsaHI Gr'CG_yC
Cuts at: 0 665 1409 1515
Size: 665 744 106
Fragments arranged by size:
744 665 106
BsaWI w'CCGG_w
Cuts at: 0 875 1515
Size: 875 640
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 710 1515
Size: 710 805
BseYI C'CCAG_C
Cuts at: 0 221 997 1351 1515
Size: 221 776 354 164
Fragments arranged by size:
776 354 221 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 237 1515
Size: 133 104 1278
Fragments arranged by size:
1278 133 104
BsiHKAI G_wGCw'C
Cuts at: 0 428 1515
Size: 428 1087
BsmI GAATG_Cn'
Cuts at: 0 368 1515
Size: 368 1147
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1065 1515
Size: 1065 450
Bsp1286I G_dGCh'C
Cuts at: 0 228 428 586 1058 1358 1515
Size: 228 200 158 472 300 157
Fragments arranged by size:
472 300 228 200 158 157
BspEI T'CCGG_A
Cuts at: 0 875 1515
Size: 875 640
BsrBI CCG'CTC
Cuts at: 0 330 339 800 1515
Size: 330 9 461 715
Fragments arranged by size:
715 461 330 9
BsrFI r'CCGG_y
Cuts at: 0 595 1315 1433 1515
Size: 595 720 118 82
Fragments arranged by size:
720 595 118 82
BsrGI T'GTAC_A
Cuts at: 0 1133 1515
Size: 1133 382
BssSI C'ACGA_G
Cuts at: 0 421 1515
Size: 421 1094
BstEII G'GTnAC_C
Cuts at: 0 492 1515
Size: 492 1023
BstYI r'GATC_y
Cuts at: 0 878 1451 1515
Size: 878 573 64
Fragments arranged by size:
878 573 64
BstZ17I GTA'TAC
Cuts at: 0 1443 1515
Size: 1443 72
Bsu36I CC'TnA_GG
Cuts at: 0 761 1515
Size: 761 754
BtgI C'CryG_G
Cuts at: 0 729 1289 1367 1515
Size: 729 560 78 148
Fragments arranged by size:
729 560 148 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 270 948 1515
Size: 270 678 567
Fragments arranged by size:
678 567 270
BtsI GCAGTG_nn'
Cuts at: 0 622 1226 1515
Size: 622 604 289
Fragments arranged by size:
622 604 289
DraIII CAC_nnn'GTG
Cuts at: 0 212 1515
Size: 212 1303
EaeI y'GGCC_r
Cuts at: 0 815 1146 1298 1515
Size: 815 331 152 217
Fragments arranged by size:
815 331 217 152
EarI CTCTTCn'nnn_
Cuts at: 0 685 836 1021 1515
Size: 685 151 185 494
Fragments arranged by size:
685 494 185 151
EcoICRI GAG'CTC
Cuts at: 0 426 1515
Size: 426 1089
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 1285 1515
Size: 30 1255 230
Fragments arranged by size:
1255 230 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 45 1515
Size: 45 1470
EcoO109I rG'GnC_Cy
Cuts at: 0 225 621 765 1002 1194 1339 1515
Size: 225 396 144 237 192 145 176
Fragments arranged by size:
396 237 225 192 176 145 144
EcoRI G'AATT_C
Cuts at: 0 552 1515
Size: 552 963
HaeII r_GCGC'y
Cuts at: 0 874 1515
Size: 874 641
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 1515
Size: 56 32 1427
Fragments arranged by size:
1427 56 32
MscI TGG'CCA
Cuts at: 0 817 1148 1515
Size: 817 331 367
Fragments arranged by size:
817 367 331
MslI CAynn'nnrTG
Cuts at: 0 301 565 604 731 983 1174 1515
Size: 301 264 39 127 252 191 341
Fragments arranged by size:
341 301 264 252 191 127 39
MspA1I CmG'CkG
Cuts at: 0 56 221 358 742 1487 1515
Size: 56 165 137 384 745 28
Fragments arranged by size:
745 384 165 137 56 28
NaeI GCC'GGC
Cuts at: 0 597 1515
Size: 597 918
NgoMIV G'CCGG_C
Cuts at: 0 595 1515
Size: 595 920
NspI r_CATG'y
Cuts at: 0 114 306 1395 1515
Size: 114 192 1089 120
Fragments arranged by size:
1089 192 120 114
PasI CC'CwG_GG
Cuts at: 0 769 1515
Size: 769 746
PciI A'CATG_T
Cuts at: 0 110 1515
Size: 110 1405
PflMI CCAn_nnn'nTGG
Cuts at: 0 479 1373 1515
Size: 479 894 142
Fragments arranged by size:
894 479 142
PfoI T'CCnGG_A
Cuts at: 0 18 115 1515
Size: 18 97 1400
Fragments arranged by size:
1400 97 18
PpuMI rG'GwC_Cy
Cuts at: 0 765 1002 1515
Size: 765 237 513
Fragments arranged by size:
765 513 237
PspOMI G'GGCC_C
Cuts at: 0 224 1515
Size: 224 1291
PvuII CAG'CTG
Cuts at: 0 56 221 358 742 1515
Size: 56 165 137 384 773
Fragments arranged by size:
773 384 165 137 56
SacI G_AGCT'C
Cuts at: 0 428 1515
Size: 428 1087
SapI GCTCTTCn'nnn_
Cuts at: 0 836 1515
Size: 836 679
SmlI C'TyrA_G
Cuts at: 0 805 1515
Size: 805 710
StuI AGG'CCT
Cuts at: 0 68 1515
Size: 68 1447
StyI C'CwwG_G
Cuts at: 0 827 989 1515
Size: 827 162 526
Fragments arranged by size:
827 526 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 594 710 1515
Size: 594 116 805
Fragments arranged by size:
805 594 116
TatI w'GTAC_w
Cuts at: 0 1133 1515
Size: 1133 382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 454 1515
Size: 454 1061
ZraI GAC'GTC
Cuts at: 0 1410 1515
Size: 1410 105
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AloI AlwNI ApaI
ApoI BamHI BanI BanII BbsI BbvCI BcgI BclI
BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BsrBI BsrFI BsrGI BssSI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MscI MslI
MspA1I NaeI NgoMIV NspI PasI PciI PflMI PfoI
PpuMI PspOMI PvuII SacI SapI SmlI StuI StyI
TaqII TatI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BciVI BfrBI BlpI BmgBI BmrI BmtI BplI BsaI
BsaAI BsaXI BsiEI BsiWI BspHI BspMI BsrDI BssHII
BstAPI BstBI BstXI ClaI CspCI DraI DrdI EagI
EciI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI