Restriction map for
pFN21AE6474
(Linear) (Six-base) MAPSORT of: KIEE6474.seq Check: 4717 from: 1 to: 1410
KIEE6474.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AccI GT'mk_AC
Cuts at: 0 1268 1410
Size: 1268 142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 712 797 1410
Size: 460 252 85 613
Fragments arranged by size:
613 460 252 85
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 141 1410
Size: 141 1269
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 696 728 1410
Size: 696 32 682
Fragments arranged by size:
696 682 32
AlwNI CAG_nnn'CTG
Cuts at: 0 820 1410
Size: 820 590
ApoI r'AATT_y
Cuts at: 0 89 437 803 1410
Size: 89 348 366 607
Fragments arranged by size:
607 366 348 89
AseI AT'TA_AT
Cuts at: 0 71 1120 1410
Size: 71 1049 290
Fragments arranged by size:
1049 290 71
BamHI G'GATC_C
Cuts at: 0 1039 1410
Size: 1039 371
BanII G_rGCy'C
Cuts at: 0 237 1410
Size: 237 1173
BfrBI ATG'CAT
Cuts at: 0 722 1410
Size: 722 688
BglI GCCn_nnn'nGGC
Cuts at: 0 175 959 1410
Size: 175 784 451
Fragments arranged by size:
784 451 175
Bme1580I G_kGCm'C
Cuts at: 0 55 1410
Size: 55 1355
BmgBI CAC'GTC
Cuts at: 0 1218 1410
Size: 1218 192
BmtI G_CTAG'C
Cuts at: 0 488 1410
Size: 488 922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1410
Size: 551 859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 235 1410
Size: 10 225 1175
Fragments arranged by size:
1175 225 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 927 1410
Size: 927 483
BsaAI yAC'GTr
Cuts at: 0 1272 1410
Size: 1272 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 250 280 1410
Size: 250 30 1130
Fragments arranged by size:
1130 250 30
BseYI C'CCAG_C
Cuts at: 0 373 1410
Size: 373 1037
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1410
Size: 104 1306
BsiHKAI G_wGCw'C
Cuts at: 0 237 1410
Size: 237 1173
BsmI GAATG_Cn'
Cuts at: 0 588 1410
Size: 588 822
Bsp1286I G_dGCh'C
Cuts at: 0 55 237 1410
Size: 55 182 1173
Fragments arranged by size:
1173 182 55
BssSI C'ACGA_G
Cuts at: 0 449 1410
Size: 449 961
BstYI r'GATC_y
Cuts at: 0 1039 1410
Size: 1039 371
BtgI C'CryG_G
Cuts at: 0 269 1410
Size: 269 1141
BtsI GCAGTG_nn'
Cuts at: 0 234 1410
Size: 234 1176
DraI TTT'AAA
Cuts at: 0 924 1326 1410
Size: 924 402 84
Fragments arranged by size:
924 402 84
EaeI y'GGCC_r
Cuts at: 0 176 327 416 1410
Size: 176 151 89 994
Fragments arranged by size:
994 176 151 89
EarI CTCTTCn'nnn_
Cuts at: 0 816 1005 1410
Size: 816 189 405
Fragments arranged by size:
816 405 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1155 1410
Size: 1155 255
EcoICRI GAG'CTC
Cuts at: 0 235 1410
Size: 235 1175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 551 712 797 1410
Size: 460 91 161 85 613
Fragments arranged by size:
613 460 161 91 85
EcoRI G'AATT_C
Cuts at: 0 437 1410
Size: 437 973
EcoRV GAT'ATC
Cuts at: 0 609 1410
Size: 609 801
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 1355 1410
Size: 276 1079 55
Fragments arranged by size:
1079 276 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 174 206 1410
Size: 174 32 1204
Fragments arranged by size:
1204 174 32
HincII GTy'rAC
Cuts at: 0 768 1410
Size: 768 642
HindIII A'AGCT_T
Cuts at: 0 216 1410
Size: 216 1194
HpaI GTT'AAC
Cuts at: 0 768 1410
Size: 768 642
MscI TGG'CCA
Cuts at: 0 178 329 418 1410
Size: 178 151 89 992
Fragments arranged by size:
992 178 151 89
MslI CAynn'nnrTG
Cuts at: 0 133 861 1216 1410
Size: 133 728 355 194
Fragments arranged by size:
728 355 194 133
MspA1I CmG'CkG
Cuts at: 0 377 966 1015 1292 1410
Size: 377 589 49 277 118
Fragments arranged by size:
589 377 277 118 49
NheI G'CTAG_C
Cuts at: 0 484 1410
Size: 484 926
NsiI A_TGCA'T
Cuts at: 0 724 1410
Size: 724 686
NspI r_CATG'y
Cuts at: 0 668 1410
Size: 668 742
PasI CC'CwG_GG
Cuts at: 0 797 1410
Size: 797 613
PflMI CCAn_nnn'nTGG
Cuts at: 0 229 425 1410
Size: 229 196 985
Fragments arranged by size:
985 229 196
PsiI TTA'TAA
Cuts at: 0 1175 1410
Size: 1175 235
PstI C_TGCA'G
Cuts at: 0 204 1410
Size: 204 1206
PvuII CAG'CTG
Cuts at: 0 377 966 1015 1410
Size: 377 589 49 395
Fragments arranged by size:
589 395 377 49
SacI G_AGCT'C
Cuts at: 0 237 1410
Size: 237 1173
SfcI C'TryA_G
Cuts at: 0 200 749 821 1410
Size: 200 549 72 589
Fragments arranged by size:
589 549 200 72
SmlI C'TyrA_G
Cuts at: 0 25 250 1410
Size: 25 225 1160
Fragments arranged by size:
1160 225 25
SphI G_CATG'C
Cuts at: 0 668 1410
Size: 668 742
SspI AAT'ATT
Cuts at: 0 1383 1410
Size: 1383 27
StyI C'CwwG_G
Cuts at: 0 636 1410
Size: 636 774
SwaI ATTT'AAAT
Cuts at: 0 924 1410
Size: 924 486
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 810 1410
Size: 810 600
TatI w'GTAC_w
Cuts at: 0 350 1410
Size: 350 1060
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 1410
Size: 80 32 1298
Fragments arranged by size:
1298 80 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 1410
Size: 111 1299
XmnI GAAnn'nnTTC
Cuts at: 0 575 1410
Size: 575 835
Enzymes that do cut:
AccI AcuI AlfI AloI AlwNI ApoI AseI BamHI
BanII BfrBI BglI Bme1580I BmgBI BmtI BpmI BpuEI
BsaI BsaAI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I
BssSI BstYI BtgI BtsI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FalI Hin4I HincII HindIII
HpaI MscI MslI MspA1I NheI NsiI NspI PasI
PflMI PsiI PstI PvuII SacI SfcI SmlI SphI
SspI StyI SwaI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI BmrI BplI Bpu10I BsaBI BsaHI BsaWI
BseRI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI
EcoNI EcoO109I FseI FspI FspAI HaeII KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NotI NruI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI Tth111I
XbaI XhoI XmaI ZraI