Restriction map for
pFN21AE6468
(Linear) (Six-base) MAPSORT of: KIEE6468.seq Check: 2634 from: 1 to: 1776
KIEE6468.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 993 1289 1776
Size: 993 296 487
Fragments arranged by size:
993 487 296
Acc65I G'GTAC_C
Cuts at: 0 332 1776
Size: 332 1444
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1621 1776
Size: 1621 155
AflII C'TTAA_G
Cuts at: 0 1477 1776
Size: 1477 299
AflIII A'CryG_T
Cuts at: 0 80 677 1445 1776
Size: 80 597 768 331
Fragments arranged by size:
768 597 331 80
BamHI G'GATC_C
Cuts at: 0 354 1776
Size: 354 1422
BanI G'GyrC_C
Cuts at: 0 223 332 562 1776
Size: 223 109 230 1214
Fragments arranged by size:
1214 230 223 109
BbeI G_GCGC'C
Cuts at: 0 227 1776
Size: 227 1549
BbsI GAAGACnn'nnnn_
Cuts at: 0 1557 1776
Size: 1557 219
BbvCI CC'TCA_GC
Cuts at: 0 1456 1776
Size: 1456 320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 759 793 1776
Size: 759 34 983
Fragments arranged by size:
983 759 34
BglII A'GATC_T
Cuts at: 0 740 1776
Size: 740 1036
Bme1580I G_kGCm'C
Cuts at: 0 1111 1776
Size: 1111 665
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1314 1776
Size: 1314 462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 463 1018 1257 1776
Size: 249 214 555 239 519
Fragments arranged by size:
555 519 249 239 214
Bpu10I CC'TnA_GC
Cuts at: 0 1456 1573 1776
Size: 1456 117 203
Fragments arranged by size:
1456 203 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1732 1776
Size: 1732 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 285 1776
Size: 285 1491
BsaAI yAC'GTr
Cuts at: 0 1446 1776
Size: 1446 330
BsaHI Gr'CG_yC
Cuts at: 0 224 424 598 1619 1776
Size: 224 200 174 1021 157
Fragments arranged by size:
1021 224 200 174 157
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 511 1473 1683 1776
Size: 424 87 962 210 93
Fragments arranged by size:
962 424 210 93 87
BseYI C'CCAG_C
Cuts at: 0 1163 1203 1221 1354 1776
Size: 1163 40 18 133 422
Fragments arranged by size:
1163 422 133 40 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1776
Size: 517 1259
BsiEI CG_ry'CG
Cuts at: 0 16 1100 1494 1776
Size: 16 1084 394 282
Fragments arranged by size:
1084 394 282 16
BsiHKAI G_wGCw'C
Cuts at: 0 620 1181 1776
Size: 620 561 595
Fragments arranged by size:
620 595 561
BsiWI C'GTAC_G
Cuts at: 0 59 1776
Size: 59 1717
BsmI GAATG_Cn'
Cuts at: 0 170 1120 1776
Size: 170 950 656
Fragments arranged by size:
950 656 170
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1420 1751 1776
Size: 1420 331 25
Fragments arranged by size:
1420 331 25
Bsp1286I G_dGCh'C
Cuts at: 0 620 1111 1181 1776
Size: 620 491 70 595
Fragments arranged by size:
620 595 491 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 993 1289 1776
Size: 993 296 487
Fragments arranged by size:
993 487 296
BsrBI CCG'CTC
Cuts at: 0 171 1776
Size: 171 1605
BsrFI r'CCGG_y
Cuts at: 0 507 1489 1776
Size: 507 982 287
Fragments arranged by size:
982 507 287
BsrGI T'GTAC_A
Cuts at: 0 674 1776
Size: 674 1102
BssSI C'ACGA_G
Cuts at: 0 1636 1776
Size: 1636 140
BstAPI GCAn_nnn'nTGC
Cuts at: 0 204 1776
Size: 204 1572
BstEII G'GTnAC_C
Cuts at: 0 388 1776
Size: 388 1388
BstXI CCAn_nnnn'nTGG
Cuts at: 0 276 996 1111 1335 1776
Size: 276 720 115 224 441
Fragments arranged by size:
720 441 276 224 115
BstYI r'GATC_y
Cuts at: 0 354 740 1776
Size: 354 386 1036
Fragments arranged by size:
1036 386 354
BtgI C'CryG_G
Cuts at: 0 65 364 651 1769 1776
Size: 65 299 287 1118 7
Fragments arranged by size:
1118 299 287 65 7
BtsI GCAGTG_nn'
Cuts at: 0 505 601 1540 1776
Size: 505 96 939 236
Fragments arranged by size:
939 505 236 96
DraIII CAC_nnn'GTG
Cuts at: 0 1703 1776
Size: 1703 73
DrdI GACnn_nn'nnGTC
Cuts at: 0 1099 1776
Size: 1099 677
EaeI y'GGCC_r
Cuts at: 0 13 704 1097 1235 1391 1491 1776
Size: 13 691 393 138 156 100 285
Fragments arranged by size:
691 393 285 156 138 100 13
EagI C'GGCC_G
Cuts at: 0 13 1097 1491 1776
Size: 13 1084 394 285
Fragments arranged by size:
1084 394 285 13
EarI CTCTTCn'nnn_
Cuts at: 0 343 1776
Size: 343 1433
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 131 1776
Size: 131 1645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 463 1018 1257 1621 1776
Size: 249 214 555 239 364 155
Fragments arranged by size:
555 364 249 239 214 155
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1000 1285 1776
Size: 1000 285 491
Fragments arranged by size:
1000 491 285
EcoO109I rG'GnC_Cy
Cuts at: 0 639 1595 1776
Size: 639 956 181
Fragments arranged by size:
956 639 181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 924 1776
Size: 924 852
HaeII r_GCGC'y
Cuts at: 0 227 514 1776
Size: 227 287 1262
Fragments arranged by size:
1262 287 227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 1776
Size: 409 32 1335
Fragments arranged by size:
1335 409 32
HincII GTy'rAC
Cuts at: 0 1320 1776
Size: 1320 456
KasI G'GCGC_C
Cuts at: 0 223 1776
Size: 223 1553
KpnI G_GTAC'C
Cuts at: 0 336 1776
Size: 336 1440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1776
Size: 830 946
MscI TGG'CCA
Cuts at: 0 706 1776
Size: 706 1070
MslI CAynn'nnrTG
Cuts at: 0 145 676 832 1156 1333 1776
Size: 145 531 156 324 177 443
Fragments arranged by size:
531 443 324 177 156 145
MspA1I CmG'CkG
Cuts at: 0 18 702 1167 1234 1776
Size: 18 684 465 67 542
Fragments arranged by size:
684 542 465 67 18
NaeI GCC'GGC
Cuts at: 0 509 1776
Size: 509 1267
NarI GG'CG_CC
Cuts at: 0 224 1776
Size: 224 1552
NgoMIV G'CCGG_C
Cuts at: 0 507 1776
Size: 507 1269
NotI GC'GGCC_GC
Cuts at: 0 13 1776
Size: 13 1763
NspI r_CATG'y
Cuts at: 0 207 681 1221 1776
Size: 207 474 540 555
Fragments arranged by size:
555 540 474 207
PasI CC'CwG_GG
Cuts at: 0 1626 1722 1776
Size: 1626 96 54
Fragments arranged by size:
1626 96 54
PciI A'CATG_T
Cuts at: 0 677 1776
Size: 677 1099
PflMI CCAn_nnn'nTGG
Cuts at: 0 385 719 1776
Size: 385 334 1057
Fragments arranged by size:
1057 385 334
PfoI T'CCnGG_A
Cuts at: 0 350 479 1776
Size: 350 129 1297
Fragments arranged by size:
1297 350 129
PpuMI rG'GwC_Cy
Cuts at: 0 1595 1776
Size: 1595 181
PstI C_TGCA'G
Cuts at: 0 1075 1776
Size: 1075 701
PvuII CAG'CTG
Cuts at: 0 702 1167 1776
Size: 702 465 609
Fragments arranged by size:
702 609 465
SanDI GG'GwC_CC
Cuts at: 0 1595 1776
Size: 1595 181
ScaI AGT'ACT
Cuts at: 0 439 1776
Size: 439 1337
SfcI C'TryA_G
Cuts at: 0 34 1071 1776
Size: 34 1037 705
Fragments arranged by size:
1037 705 34
SfoI GGC'GCC
Cuts at: 0 225 1776
Size: 225 1551
SmlI C'TyrA_G
Cuts at: 0 1477 1747 1776
Size: 1477 270 29
Fragments arranged by size:
1477 270 29
SphI G_CATG'C
Cuts at: 0 207 1221 1776
Size: 207 1014 555
Fragments arranged by size:
1014 555 207
StuI AGG'CCT
Cuts at: 0 718 1776
Size: 718 1058
StyI C'CwwG_G
Cuts at: 0 713 1286 1776
Size: 713 573 490
Fragments arranged by size:
713 573 490
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 751 1776
Size: 751 1025
TatI w'GTAC_w
Cuts at: 0 299 437 674 1776
Size: 299 138 237 1102
Fragments arranged by size:
1102 299 237 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 579 1149 1302 1776
Size: 579 570 153 474
Fragments arranged by size:
579 570 474 153
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII BamHI BanI BbeI
BbsI BbvCI BcgI BglII Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PciI PflMI PfoI PpuMI PstI PvuII SanDI
ScaI SfcI SfoI SmlI SphI StuI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanII BciVI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BsaBI BsaWI BsaXI BspEI
BspHI BsrDI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI EcoICRI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI MfeI MluI NcoI NdeI NheI NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI