Restriction map for
pFN21AE6457
(Linear) (Six-base) MAPSORT of: KIEE6457.seq Check: 5639 from: 1 to: 1428
KIEE6457.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
Acc65I G'GTAC_C
Cuts at: 0 881 1428
Size: 881 547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1428
Size: 1207 221
AflIII A'CryG_T
Cuts at: 0 1179 1428
Size: 1179 249
AhdI GACnn_n'nnGTC
Cuts at: 0 294 1428
Size: 294 1134
ApoI r'AATT_y
Cuts at: 0 174 253 270 565 813 1288 1428
Size: 174 79 17 295 248 475 140
Fragments arranged by size:
475 295 248 174 140 79 17
AseI AT'TA_AT
Cuts at: 0 478 1428
Size: 478 950
BanI G'GyrC_C
Cuts at: 0 881 1428
Size: 881 547
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 1428
Size: 197 1231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 149 1428
Size: 115 34 1279
Fragments arranged by size:
1279 115 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 356 915 1428
Size: 356 559 513
Fragments arranged by size:
559 513 356
BglII A'GATC_T
Cuts at: 0 17 1428
Size: 17 1411
Bme1580I G_kGCm'C
Cuts at: 0 1258 1428
Size: 1258 170
Bpu10I CC'TnA_GC
Cuts at: 0 426 604 1428
Size: 426 178 824
Fragments arranged by size:
824 426 178
BsaBI GATnn'nnATC
Cuts at: 0 22 1428
Size: 22 1406
BsaWI w'CCGG_w
Cuts at: 0 7 1428
Size: 7 1421
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 49 79 397 427 1428
Size: 49 30 318 30 1001
Fragments arranged by size:
1001 318 49 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 317 1046 1280 1370 1428
Size: 317 729 234 90 58
Fragments arranged by size:
729 317 234 90 58
BseYI C'CCAG_C
Cuts at: 0 449 1428
Size: 449 979
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 1428
Size: 402 1026
BsiHKAI G_wGCw'C
Cuts at: 0 1272 1428
Size: 1272 156
BsmI GAATG_Cn'
Cuts at: 0 237 1428
Size: 237 1191
Bsp1286I G_dGCh'C
Cuts at: 0 1258 1272 1428
Size: 1258 14 156
Fragments arranged by size:
1258 156 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 642 962 1428
Size: 642 320 466
Fragments arranged by size:
642 466 320
BsrDI GCAATG_nn'
Cuts at: 0 442 1428
Size: 442 986
BstAPI GCAn_nnn'nTGC
Cuts at: 0 416 1428
Size: 416 1012
BstYI r'GATC_y
Cuts at: 0 17 1040 1428
Size: 17 1023 388
Fragments arranged by size:
1023 388 17
BtgI C'CryG_G
Cuts at: 0 962 1428
Size: 962 466
BtsI GCAGTG_nn'
Cuts at: 0 740 1428
Size: 740 688
ClaI AT'CG_AT
Cuts at: 0 866 1428
Size: 866 562
DraI TTT'AAA
Cuts at: 0 564 1428
Size: 564 864
EaeI y'GGCC_r
Cuts at: 0 794 1428
Size: 794 634
EarI CTCTTCn'nnn_
Cuts at: 0 305 377 1077 1428
Size: 305 72 700 351
Fragments arranged by size:
700 351 305 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1428
Size: 1207 221
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2 34 280 312 1428
Size: 2 32 246 32 1116
Fragments arranged by size:
1116 246 32 32 2
HincII GTy'rAC
Cuts at: 0 373 1428
Size: 373 1055
HindIII A'AGCT_T
Cuts at: 0 1219 1428
Size: 1219 209
KpnI G_GTAC'C
Cuts at: 0 885 1428
Size: 885 543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 1394 1428
Size: 1100 294 34
Fragments arranged by size:
1100 294 34
NcoI C'CATG_G
Cuts at: 0 962 1428
Size: 962 466
NspI r_CATG'y
Cuts at: 0 425 457 917 1000 1183 1234 1428
Size: 425 32 460 83 183 51 194
Fragments arranged by size:
460 425 194 183 83 51 32
PciI A'CATG_T
Cuts at: 0 1179 1428
Size: 1179 249
PflMI CCAn_nnn'nTGG
Cuts at: 0 1310 1428
Size: 1310 118
PsiI TTA'TAA
Cuts at: 0 674 1160 1428
Size: 674 486 268
Fragments arranged by size:
674 486 268
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 449 481 1428
Size: 449 32 947
Fragments arranged by size:
947 449 32
SfcI C'TryA_G
Cuts at: 0 41 168 1428
Size: 41 127 1260
Fragments arranged by size:
1260 127 41
SphI G_CATG'C
Cuts at: 0 917 1000 1428
Size: 917 83 428
Fragments arranged by size:
917 428 83
StyI C'CwwG_G
Cuts at: 0 156 340 573 962 1428
Size: 156 184 233 389 466
Fragments arranged by size:
466 389 233 184 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 776 1428
Size: 776 652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 747 1428
Size: 747 681
Enzymes that do cut:
Acc65I AcuI AflIII AhdI ApoI AseI BanI BbsI
BcgI BciVI BglII Bme1580I Bpu10I BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BstAPI BstYI BtgI BtsI ClaI DraI EaeI EarI
Eco57MI Hin4I HincII HindIII KpnI MmeI NcoI NspI
PciI PflMI PsiI PsrI SfcI SphI StyI TaqII
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI MfeI MluI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI