Restriction map for
pFN21AE6455
(Linear) (Six-base) MAPSORT of: KIEE6455.seq Check: 8204 from: 1 to: 1707
KIEE6455.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AatII G_ACGT'C
Cuts at: 0 1685 1707
Size: 1685 22
AccI GT'mk_AC
Cuts at: 0 671 1707
Size: 671 1036
AclI AA'CG_TT
Cuts at: 0 1221 1707
Size: 1221 486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 1490 1707
Size: 588 902 217
Fragments arranged by size:
902 588 217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 761 1707
Size: 761 946
ApaI G_GGCC'C
Cuts at: 0 212 406 1707
Size: 212 194 1301
Fragments arranged by size:
1301 212 194
ApoI r'AATT_y
Cuts at: 0 1611 1707
Size: 1611 96
AvaI C'yCGr_G
Cuts at: 0 70 217 399 476 658 1707
Size: 70 147 182 77 182 1049
Fragments arranged by size:
1049 182 182 147 77 70
AvrII C'CTAG_G
Cuts at: 0 762 1707
Size: 762 945
BamHI G'GATC_C
Cuts at: 0 892 1707
Size: 892 815
BanI G'GyrC_C
Cuts at: 0 244 441 701 758 1707
Size: 244 197 260 57 949
Fragments arranged by size:
949 260 244 197 57
BanII G_rGCy'C
Cuts at: 0 212 406 420 1707
Size: 212 194 14 1287
Fragments arranged by size:
1287 212 194 14
BbeI G_GCGC'C
Cuts at: 0 248 445 762 1707
Size: 248 197 317 945
Fragments arranged by size:
945 317 248 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 1380 1707
Size: 1380 327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 15 49 273 307 1707
Size: 15 34 224 34 1400
Fragments arranged by size:
1400 224 34 34 15
BfrBI ATG'CAT
Cuts at: 0 1540 1573 1707
Size: 1540 33 134
Fragments arranged by size:
1540 134 33
BglI GCCn_nnn'nGGC
Cuts at: 0 253 661 1707
Size: 253 408 1046
Fragments arranged by size:
1046 408 253
BlpI GC'TnA_GC
Cuts at: 0 158 526 1707
Size: 158 368 1181
Fragments arranged by size:
1181 368 158
Bme1580I G_kGCm'C
Cuts at: 0 212 406 1707
Size: 212 194 1301
Fragments arranged by size:
1301 212 194
BmrI ACTGGGnnnn_n'
Cuts at: 0 206 1707
Size: 206 1501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 408 1269 1707
Size: 127 281 861 438
Fragments arranged by size:
861 438 281 127
Bpu10I CC'TnA_GC
Cuts at: 0 553 1707
Size: 553 1154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1707
Size: 817 890
BsaI GGTCTCn'nnnn_
Cuts at: 0 226 1707
Size: 226 1481
BsaHI Gr'CG_yC
Cuts at: 0 245 442 625 759 1682 1707
Size: 245 197 183 134 923 25
Fragments arranged by size:
923 245 197 183 134 25
BseYI C'CCAG_C
Cuts at: 0 65 540 681 1707
Size: 65 475 141 1026
Fragments arranged by size:
1026 475 141 65
BsiEI CG_ry'CG
Cuts at: 0 343 671 1707
Size: 343 328 1036
Fragments arranged by size:
1036 343 328
BsiHKAI G_wGCw'C
Cuts at: 0 794 1707
Size: 794 913
Bsp1286I G_dGCh'C
Cuts at: 0 212 406 420 794 1707
Size: 212 194 14 374 913
Fragments arranged by size:
913 374 212 194 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 260 1707
Size: 260 1447
BsrBI CCG'CTC
Cuts at: 0 98 422 734 1707
Size: 98 324 312 973
Fragments arranged by size:
973 324 312 98
BsrDI GCAATG_nn'
Cuts at: 0 137 1575 1707
Size: 137 1438 132
Fragments arranged by size:
1438 137 132
BsrFI r'CCGG_y
Cuts at: 0 294 338 698 1707
Size: 294 44 360 1009
Fragments arranged by size:
1009 360 294 44
BstXI CCAn_nnnn'nTGG
Cuts at: 0 853 1707
Size: 853 854
BstYI r'GATC_y
Cuts at: 0 892 1707
Size: 892 815
BtgI C'CryG_G
Cuts at: 0 436 569 665 715 1707
Size: 436 133 96 50 992
Fragments arranged by size:
992 436 133 96 50
BtsI GCAGTG_nn'
Cuts at: 0 28 118 1707
Size: 28 90 1589
Fragments arranged by size:
1589 90 28
EaeI y'GGCC_r
Cuts at: 0 254 296 317 329 340 572 650 839
Size: 254 42 21 12 11 232 78 189
Cuts at: 839 855 1707
Size: 16 852
Fragments arranged by size:
852 254 232 189 78 42 21 16
12 11
EagI C'GGCC_G
Cuts at: 0 340 1707
Size: 340 1367
EarI CTCTTCn'nnn_
Cuts at: 0 85 1707
Size: 85 1622
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 372 509 1707
Size: 372 137 1198
Fragments arranged by size:
1198 372 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 408 588 1269 1490 1707
Size: 127 281 180 681 221 217
Fragments arranged by size:
681 281 221 217 180 127
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 832 1707
Size: 256 576 875
Fragments arranged by size:
875 576 256
EcoO109I rG'GnC_Cy
Cuts at: 0 208 354 549 645 1131 1275 1707
Size: 208 146 195 96 486 144 432
Fragments arranged by size:
486 432 208 195 146 144 96
EcoRI G'AATT_C
Cuts at: 0 1611 1707
Size: 1611 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 702 1707
Size: 702 1005
FspI TGC'GCA
Cuts at: 0 268 1707
Size: 268 1439
HaeII r_GCGC'y
Cuts at: 0 248 432 445 598 762 1707
Size: 248 184 13 153 164 945
Fragments arranged by size:
945 248 184 164 153 13
HincII GTy'rAC
Cuts at: 0 672 1428 1707
Size: 672 756 279
Fragments arranged by size:
756 672 279
KasI G'GCGC_C
Cuts at: 0 244 441 758 1707
Size: 244 197 317 949
Fragments arranged by size:
949 317 244 197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 920 1707
Size: 49 871 787
Fragments arranged by size:
871 787 49
MscI TGG'CCA
Cuts at: 0 857 1707
Size: 857 850
MspA1I CmG'CkG
Cuts at: 0 162 279 302 438 447 582 630 667
Size: 162 117 23 136 9 135 48 37
Cuts at: 667 717 1085 1707
Size: 50 368 622
Fragments arranged by size:
622 368 162 136 135 117 50 48
37 23 9
NaeI GCC'GGC
Cuts at: 0 296 700 1707
Size: 296 404 1007
Fragments arranged by size:
1007 404 296
NarI GG'CG_CC
Cuts at: 0 245 442 759 1707
Size: 245 197 317 948
Fragments arranged by size:
948 317 245 197
NgoMIV G'CCGG_C
Cuts at: 0 294 698 1707
Size: 294 404 1009
Fragments arranged by size:
1009 404 294
NsiI A_TGCA'T
Cuts at: 0 1542 1575 1707
Size: 1542 33 132
Fragments arranged by size:
1542 132 33
NspI r_CATG'y
Cuts at: 0 366 1707
Size: 366 1341
PflMI CCAn_nnn'nTGG
Cuts at: 0 864 1707
Size: 864 843
PfoI T'CCnGG_A
Cuts at: 0 356 1707
Size: 356 1351
PpuMI rG'GwC_Cy
Cuts at: 0 354 645 1131 1707
Size: 354 291 486 576
Fragments arranged by size:
576 486 354 291
PspOMI G'GGCC_C
Cuts at: 0 208 402 1707
Size: 208 194 1305
Fragments arranged by size:
1305 208 194
PvuII CAG'CTG
Cuts at: 0 1085 1707
Size: 1085 622
SacII CC_GC'GG
Cuts at: 0 439 668 718 1707
Size: 439 229 50 989
Fragments arranged by size:
989 439 229 50
SalI G'TCGA_C
Cuts at: 0 670 1707
Size: 670 1037
SanDI GG'GwC_CC
Cuts at: 0 354 1131 1707
Size: 354 777 576
Fragments arranged by size:
777 576 354
ScaI AGT'ACT
Cuts at: 0 1442 1707
Size: 1442 265
SfcI C'TryA_G
Cuts at: 0 882 1707
Size: 882 825
SfoI GGC'GCC
Cuts at: 0 246 443 760 1707
Size: 246 197 317 947
Fragments arranged by size:
947 317 246 197
SmaI CCC'GGG
Cuts at: 0 401 478 660 1707
Size: 401 77 182 1047
Fragments arranged by size:
1047 401 182 77
SmlI C'TyrA_G
Cuts at: 0 832 1707
Size: 832 875
StuI AGG'CCT
Cuts at: 0 508 1707
Size: 508 1199
StyI C'CwwG_G
Cuts at: 0 239 762 1707
Size: 239 523 945
Fragments arranged by size:
945 523 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 1385 1707
Size: 79 1306 322
Fragments arranged by size:
1306 322 79
TatI w'GTAC_w
Cuts at: 0 1440 1707
Size: 1440 267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 1147 1707
Size: 306 841 560
Fragments arranged by size:
841 560 306
XmaI C'CCGG_G
Cuts at: 0 399 476 658 1707
Size: 399 77 182 1049
Fragments arranged by size:
1049 399 182 77
XmnI GAAnn'nnTTC
Cuts at: 0 1328 1475 1707
Size: 1328 147 232
Fragments arranged by size:
1328 232 147
ZraI GAC'GTC
Cuts at: 0 1683 1707
Size: 1683 24
Enzymes that do cut:
AatII AccI AclI AcuI AlfI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BfrBI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstXI BstYI BtgI BtsI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
HincII KasI MmeI MscI MspA1I NaeI NarI NgoMIV
NsiI NspI PflMI PfoI PpuMI PspOMI PvuII SacII
SalI SanDI ScaI SfcI SfoI SmaI SmlI StuI
StyI TaqII TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AflIII AgeI AhdI AleI
AloI AlwNI ApaLI AscI AseI AsiSI BaeI BbvCI
BciVI BclI BglII BmgBI BmtI BplI BsaAI BsaBI
BsaWI BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoRV FseI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MslI NcoI NdeI NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SapI SbfI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI