Restriction map for
pFN21AE6450
(Linear) (Six-base) MAPSORT of: KIEE6450.seq Check: 5719 from: 1 to: 1581
KIEE6450.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
Acc65I G'GTAC_C
Cuts at: 0 555 1581
Size: 555 1026
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 1581
Size: 13 1568
AfeI AGC'GCT
Cuts at: 0 1275 1581
Size: 1275 306
AflIII A'CryG_T
Cuts at: 0 662 1581
Size: 662 919
AhdI GACnn_n'nnGTC
Cuts at: 0 609 1581
Size: 609 972
ApaLI G'TGCA_C
Cuts at: 0 170 1581
Size: 170 1411
ApoI r'AATT_y
Cuts at: 0 491 633 755 787 874 1334 1546 1581
Size: 491 142 122 32 87 460 212 35
Fragments arranged by size:
491 460 212 142 122 87 35 32
AseI AT'TA_AT
Cuts at: 0 1292 1581
Size: 1292 289
BanI G'GyrC_C
Cuts at: 0 225 555 1581
Size: 225 330 1026
Fragments arranged by size:
1026 330 225
BanII G_rGCy'C
Cuts at: 0 128 889 1581
Size: 128 761 692
Fragments arranged by size:
761 692 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 723 1368 1581
Size: 723 645 213
Fragments arranged by size:
723 645 213
BbvCI CC'TCA_GC
Cuts at: 0 969 1581
Size: 969 612
BciVI GTATCCnnnnn_n'
Cuts at: 0 1055 1581
Size: 1055 526
BclI T'GATC_A
Cuts at: 0 1185 1344 1581
Size: 1185 159 237
Fragments arranged by size:
1185 237 159
BglII A'GATC_T
Cuts at: 0 371 1581
Size: 371 1210
BlpI GC'TnA_GC
Cuts at: 0 1270 1581
Size: 1270 311
Bme1580I G_kGCm'C
Cuts at: 0 174 230 1581
Size: 174 56 1351
Fragments arranged by size:
1351 174 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 536 1581
Size: 536 1045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1581
Size: 506 1075
Bpu10I CC'TnA_GC
Cuts at: 0 888 969 1581
Size: 888 81 612
Fragments arranged by size:
888 612 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1581
Size: 531 1050
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1214 1244 1581
Size: 1214 30 337
Fragments arranged by size:
1214 337 30
BsiHKAI G_wGCw'C
Cuts at: 0 128 174 889 1581
Size: 128 46 715 692
Fragments arranged by size:
715 692 128 46
BsmI GAATG_Cn'
Cuts at: 0 1320 1581
Size: 1320 261
Bsp1286I G_dGCh'C
Cuts at: 0 128 174 230 889 1581
Size: 128 46 56 659 692
Fragments arranged by size:
692 659 128 56 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 852 1581
Size: 852 729
BsrDI GCAATG_nn'
Cuts at: 0 1199 1581
Size: 1199 382
BstYI r'GATC_y
Cuts at: 0 371 1130 1581
Size: 371 759 451
Fragments arranged by size:
759 451 371
Bsu36I CC'TnA_GG
Cuts at: 0 860 1581
Size: 860 721
BtgI C'CryG_G
Cuts at: 0 1149 1581
Size: 1149 432
BtsI GCAGTG_nn'
Cuts at: 0 834 1581
Size: 834 747
DraI TTT'AAA
Cuts at: 0 482 1581
Size: 482 1099
DraIII CAC_nnn'GTG
Cuts at: 0 360 1581
Size: 360 1221
EaeI y'GGCC_r
Cuts at: 0 76 332 1581
Size: 76 256 1249
Fragments arranged by size:
1249 256 76
EcoICRI GAG'CTC
Cuts at: 0 126 887 1581
Size: 126 761 694
Fragments arranged by size:
761 694 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 506 1581
Size: 13 493 1075
Fragments arranged by size:
1075 493 13
EcoRI G'AATT_C
Cuts at: 0 491 1334 1581
Size: 491 843 247
Fragments arranged by size:
843 491 247
HaeII r_GCGC'y
Cuts at: 0 1277 1581
Size: 1277 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 306 338 595 627 914 946 1581
Size: 306 32 257 32 287 32 635
Fragments arranged by size:
635 306 287 257 32 32 32
HindIII A'AGCT_T
Cuts at: 0 707 1100 1581
Size: 707 393 481
Fragments arranged by size:
707 481 393
KpnI G_GTAC'C
Cuts at: 0 559 1581
Size: 559 1022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 1581
Size: 804 777
MscI TGG'CCA
Cuts at: 0 78 334 1581
Size: 78 256 1247
Fragments arranged by size:
1247 256 78
MspA1I CmG'CkG
Cuts at: 0 402 1228 1581
Size: 402 826 353
Fragments arranged by size:
826 402 353
NspI r_CATG'y
Cuts at: 0 666 1581
Size: 666 915
PciI A'CATG_T
Cuts at: 0 662 1581
Size: 662 919
PflMI CCAn_nnn'nTGG
Cuts at: 0 1464 1581
Size: 1464 117
PsiI TTA'TAA
Cuts at: 0 1307 1581
Size: 1307 274
PvuII CAG'CTG
Cuts at: 0 402 1228 1581
Size: 402 826 353
Fragments arranged by size:
826 402 353
SacI G_AGCT'C
Cuts at: 0 128 889 1581
Size: 128 761 692
Fragments arranged by size:
761 692 128
SexAI A'CCwGG_T
Cuts at: 0 1444 1581
Size: 1444 137
SfcI C'TryA_G
Cuts at: 0 652 699 1581
Size: 652 47 882
Fragments arranged by size:
882 652 47
SmlI C'TyrA_G
Cuts at: 0 510 1581
Size: 510 1071
TatI w'GTAC_w
Cuts at: 0 503 1581
Size: 503 1078
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1214 1246 1581
Size: 1214 32 335
Fragments arranged by size:
1214 335 32
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AhdI ApaLI ApoI AseI
BanI BanII BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaXI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BstYI Bsu36I BtgI BtsI DraI
DraIII EaeI EcoICRI Eco57MI EcoRI HaeII Hin4I HindIII
KpnI MmeI MscI MspA1I NspI PciI PflMI PsiI
PvuII SacI SexAI SfcI SmlI TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AleI AlfI
AloI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BcgI BfrBI BglI BmgBI BmrI BmtI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DrdI EagI EarI EciI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI