Restriction map for
pFN21AE6449
(Linear) (Six-base) MAPSORT of: KIEE6449.seq Check: 6945 from: 1 to: 1602
KIEE6449.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 428 1430 1602
Size: 428 1002 172
Fragments arranged by size:
1002 428 172
Acc65I G'GTAC_C
Cuts at: 0 1451 1602
Size: 1451 151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1283 1602
Size: 1283 319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 440 1602
Size: 440 1162
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2 34 1602
Size: 2 32 1568
Fragments arranged by size:
1568 32 2
AlwNI CAG_nnn'CTG
Cuts at: 0 119 1602
Size: 119 1483
ApoI r'AATT_y
Cuts at: 0 262 325 868 941 1602
Size: 262 63 543 73 661
Fragments arranged by size:
661 543 262 73 63
AscI GG'CGCG_CC
Cuts at: 0 1412 1602
Size: 1412 190
AseI AT'TA_AT
Cuts at: 0 884 1602
Size: 884 718
BanI G'GyrC_C
Cuts at: 0 99 416 1451 1602
Size: 99 317 1035 151
Fragments arranged by size:
1035 317 151 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 813 1602
Size: 813 789
BclI T'GATC_A
Cuts at: 0 365 410 1188 1602
Size: 365 45 778 414
Fragments arranged by size:
778 414 365 45
BfrBI ATG'CAT
Cuts at: 0 754 1602
Size: 754 848
BlpI GC'TnA_GC
Cuts at: 0 124 1602
Size: 124 1478
Bme1580I G_kGCm'C
Cuts at: 0 104 553 1602
Size: 104 449 1049
Fragments arranged by size:
1049 449 104
BmgBI CAC'GTC
Cuts at: 0 1473 1602
Size: 1473 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 914 1250 1317 1602
Size: 208 706 336 67 285
Fragments arranged by size:
706 336 285 208 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 1602
Size: 537 1065
BsaI GGTCTCn'nnnn_
Cuts at: 0 939 1602
Size: 939 663
BsaWI w'CCGG_w
Cuts at: 0 1544 1602
Size: 1544 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 556 586 1217 1247 1602
Size: 556 30 631 30 355
Fragments arranged by size:
631 556 355 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 1602
Size: 46 1556
BseYI C'CCAG_C
Cuts at: 0 279 1602
Size: 279 1323
Bsp1286I G_dGCh'C
Cuts at: 0 104 553 1602
Size: 104 449 1049
Fragments arranged by size:
1049 449 104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 428 1430 1602
Size: 428 1002 172
Fragments arranged by size:
1002 428 172
BsrDI GCAATG_nn'
Cuts at: 0 1158 1602
Size: 1158 444
BsrGI T'GTAC_A
Cuts at: 0 200 1602
Size: 200 1402
BssHII G'CGCG_C
Cuts at: 0 1412 1602
Size: 1412 190
BstAPI GCAn_nnn'nTGC
Cuts at: 0 700 1602
Size: 700 902
BstEII G'GTnAC_C
Cuts at: 0 1087 1602
Size: 1087 515
BstYI r'GATC_y
Cuts at: 0 738 1602
Size: 738 864
BtgI C'CryG_G
Cuts at: 0 1128 1163 1602
Size: 1128 35 439
Fragments arranged by size:
1128 439 35
DraI TTT'AAA
Cuts at: 0 1524 1602
Size: 1524 78
DraIII CAC_nnn'GTG
Cuts at: 0 171 557 1602
Size: 171 386 1045
Fragments arranged by size:
1045 386 171
EaeI y'GGCC_r
Cuts at: 0 2 35 1373 1490 1602
Size: 2 33 1338 117 112
Fragments arranged by size:
1338 117 112 33 2
EarI CTCTTCn'nnn_
Cuts at: 0 206 1602
Size: 206 1396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 914 1250 1283 1317 1602
Size: 208 706 336 33 34 285
Fragments arranged by size:
706 336 285 208 34 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1342 1602
Size: 1342 260
EcoO109I rG'GnC_Cy
Cuts at: 0 147 405 1602
Size: 147 258 1197
Fragments arranged by size:
1197 258 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 917 949 1602
Size: 917 32 653
Fragments arranged by size:
917 653 32
KpnI G_GTAC'C
Cuts at: 0 1455 1602
Size: 1455 147
MfeI C'AATT_G
Cuts at: 0 965 1602
Size: 965 637
MscI TGG'CCA
Cuts at: 0 4 37 1602
Size: 4 33 1565
Fragments arranged by size:
1565 33 4
MslI CAynn'nnrTG
Cuts at: 0 422 759 1168 1602
Size: 422 337 409 434
Fragments arranged by size:
434 422 409 337
MspA1I CmG'CkG
Cuts at: 0 33 1365 1602
Size: 33 1332 237
Fragments arranged by size:
1332 237 33
NcoI C'CATG_G
Cuts at: 0 1128 1163 1602
Size: 1128 35 439
Fragments arranged by size:
1128 439 35
NdeI CA'TA_TG
Cuts at: 0 1147 1602
Size: 1147 455
NsiI A_TGCA'T
Cuts at: 0 756 1602
Size: 756 846
NspI r_CATG'y
Cuts at: 0 132 427 642 1602
Size: 132 295 215 960
Fragments arranged by size:
960 295 215 132
PasI CC'CwG_GG
Cuts at: 0 71 1602
Size: 71 1531
PflMI CCAn_nnn'nTGG
Cuts at: 0 171 174 248 1467 1602
Size: 171 3 74 1219 135
Fragments arranged by size:
1219 171 135 74 3
PpuMI rG'GwC_Cy
Cuts at: 0 147 1602
Size: 147 1455
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 628 660 1602
Size: 628 32 942
Fragments arranged by size:
942 628 32
PvuII CAG'CTG
Cuts at: 0 33 1602
Size: 33 1569
SexAI A'CCwGG_T
Cuts at: 0 167 1447 1602
Size: 167 1280 155
Fragments arranged by size:
1280 167 155
SmlI C'TyrA_G
Cuts at: 0 516 1602
Size: 516 1086
SphI G_CATG'C
Cuts at: 0 132 427 1602
Size: 132 295 1175
Fragments arranged by size:
1175 295 132
StuI AGG'CCT
Cuts at: 0 139 1602
Size: 139 1463
StyI C'CwwG_G
Cuts at: 0 1128 1163 1466 1602
Size: 1128 35 303 136
Fragments arranged by size:
1128 303 136 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1129 1374 1602
Size: 1129 245 228
Fragments arranged by size:
1129 245 228
TatI w'GTAC_w
Cuts at: 0 200 1602
Size: 200 1402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 543 575 1217 1249 1602
Size: 543 32 642 32 353
Fragments arranged by size:
642 543 353 32 32
XbaI T'CTAG_A
Cuts at: 0 331 1602
Size: 331 1271
XmnI GAAnn'nnTTC
Cuts at: 0 220 314 1602
Size: 220 94 1288
Fragments arranged by size:
1288 220 94
Enzymes that do cut:
AarI Acc65I AcuI AlfI AloI AlwNI ApoI AscI
AseI BanI BbsI BclI BfrBI BlpI Bme1580I BmgBI
BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI Bsp1286I
BspMI BsrDI BsrGI BssHII BstAPI BstEII BstYI BtgI
DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I
KpnI MfeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PasI PflMI PpuMI PsrI PvuII SexAI SmlI
SphI StuI StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI ApaI ApaLI AsiSI AvaI AvrII BaeI BamHI
BanII BbeI BbvCI BcgI BciVI BglI BglII BmrI
BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MluI MmeI NaeI NarI NgoMIV NheI NotI NruI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I
XcmI XhoI XmaI ZraI