Restriction map for
pFN21AE6448
(Linear) (Six-base) MAPSORT of: KIEE6448.seq Check: 4110 from: 1 to: 1623
KIEE6448.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1435 1623
Size: 1435 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 514 815 1623
Size: 83 431 301 808
Fragments arranged by size:
808 431 301 83
AhdI GACnn_n'nnGTC
Cuts at: 0 261 1623
Size: 261 1362
AleI CACnn'nnGTG
Cuts at: 0 469 1148 1316 1623
Size: 469 679 168 307
Fragments arranged by size:
679 469 307 168
AlwNI CAG_nnn'CTG
Cuts at: 0 129 1623
Size: 129 1494
ApaI G_GGCC'C
Cuts at: 0 93 425 692 1055 1063 1533 1573 1623
Size: 93 332 267 363 8 470 40 50
Fragments arranged by size:
470 363 332 267 93 50 40 8
AvaI C'yCGr_G
Cuts at: 0 85 483 1565 1623
Size: 85 398 1082 58
Fragments arranged by size:
1082 398 85 58
BanI G'GyrC_C
Cuts at: 0 780 844 1039 1214 1623
Size: 780 64 195 175 409
Fragments arranged by size:
780 409 195 175 64
BanII G_rGCy'C
Cuts at: 0 93 425 692 1055 1063 1533 1564 1573
Size: 93 332 267 363 8 470 31 9
Cuts at: 1573 1623
Size: 50
Fragments arranged by size:
470 363 332 267 93 50 31 9
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 71 1144 1623
Size: 71 1073 479
Fragments arranged by size:
1073 479 71
BbvCI CC'TCA_GC
Cuts at: 0 558 1623
Size: 558 1065
BciVI GTATCCnnnnn_n'
Cuts at: 0 51 1623
Size: 51 1572
BglI GCCn_nnn'nGGC
Cuts at: 0 438 1623
Size: 438 1185
BlpI GC'TnA_GC
Cuts at: 0 250 1623
Size: 250 1373
Bme1580I G_kGCm'C
Cuts at: 0 93 282 425 692 785 1055 1063 1255
Size: 93 189 143 267 93 270 8 192
Cuts at: 1255 1533 1573 1623
Size: 278 40 50
Fragments arranged by size:
278 270 267 192 189 143 93 93
50 40 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 749 1058 1458 1623
Size: 749 309 400 165
Fragments arranged by size:
749 400 309 165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 219 1623
Size: 219 1404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 988 1052 1623
Size: 151 837 64 571
Fragments arranged by size:
837 571 151 64
Bpu10I CC'TnA_GC
Cuts at: 0 558 1623
Size: 558 1065
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1623
Size: 1276 347
BsaI GGTCTCn'nnnn_
Cuts at: 0 1513 1623
Size: 1513 110
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 169 319 689 985 1037 1623
Size: 154 15 150 370 296 52 586
Fragments arranged by size:
586 370 296 154 150 52 15
BseYI C'CCAG_C
Cuts at: 0 351 563 990 1526 1623
Size: 351 212 427 536 97
Fragments arranged by size:
536 427 351 212 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 1623
Size: 447 1176
BsiEI CG_ry'CG
Cuts at: 0 276 1623
Size: 276 1347
BsiHKAI G_wGCw'C
Cuts at: 0 1564 1623
Size: 1564 59
Bsp1286I G_dGCh'C
Cuts at: 0 93 282 425 692 785 1055 1063 1255
Size: 93 189 143 267 93 270 8 192
Cuts at: 1255 1533 1564 1573 1623
Size: 278 31 9 50
Fragments arranged by size:
278 270 267 192 189 143 93 93
50 31 9 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 952 1435 1623
Size: 952 483 188
Fragments arranged by size:
952 483 188
BstAPI GCAn_nnn'nTGC
Cuts at: 0 286 1623
Size: 286 1337
BstXI CCAn_nnnn'nTGG
Cuts at: 0 367 1122 1623
Size: 367 755 501
Fragments arranged by size:
755 501 367
BstYI r'GATC_y
Cuts at: 0 126 1623
Size: 126 1497
Bsu36I CC'TnA_GG
Cuts at: 0 661 1623
Size: 661 962
BtgI C'CryG_G
Cuts at: 0 436 1439 1485 1623
Size: 436 1003 46 138
Fragments arranged by size:
1003 436 138 46
BtsI GCAGTG_nn'
Cuts at: 0 627 916 1623
Size: 627 289 707
Fragments arranged by size:
707 627 289
DraIII CAC_nnn'GTG
Cuts at: 0 286 841 1147 1623
Size: 286 555 306 476
Fragments arranged by size:
555 476 306 286
EaeI y'GGCC_r
Cuts at: 0 273 540 1283 1482 1623
Size: 273 267 743 199 141
Fragments arranged by size:
743 273 267 199 141
EagI C'GGCC_G
Cuts at: 0 273 1623
Size: 273 1350
EarI CTCTTCn'nnn_
Cuts at: 0 140 146 1623
Size: 140 6 1477
Fragments arranged by size:
1477 140 6
EcoICRI GAG'CTC
Cuts at: 0 1562 1623
Size: 1562 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 151 514 815 988 1052 1623
Size: 83 68 363 301 173 64 571
Fragments arranged by size:
571 363 301 173 83 68 64
EcoO109I rG'GnC_Cy
Cuts at: 0 82 89 90 421 422 688 689 891
Size: 82 7 1 331 1 266 1 202
Cuts at: 891 1051 1052 1059 1060 1501 1530 1569 1623
Size: 160 1 7 1 441 29 39 54
Fragments arranged by size:
441 331 266 202 160 82 54 39
29 7 7 1 1 1 1 1
HaeII r_GCGC'y
Cuts at: 0 1014 1467 1623
Size: 1014 453 156
Fragments arranged by size:
1014 453 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 654 686 1623
Size: 247 32 375 32 937
Fragments arranged by size:
937 375 247 32 32
HincII GTy'rAC
Cuts at: 0 573 1623
Size: 573 1050
MscI TGG'CCA
Cuts at: 0 1285 1484 1623
Size: 1285 199 139
Fragments arranged by size:
1285 199 139
MslI CAynn'nnrTG
Cuts at: 0 469 1148 1316 1623
Size: 469 679 168 307
Fragments arranged by size:
679 469 307 168
MspA1I CmG'CkG
Cuts at: 0 363 518 536 545 620 725 873 1166
Size: 363 155 18 9 75 105 148 293
Cuts at: 1166 1175 1355 1418 1623
Size: 9 180 63 205
Fragments arranged by size:
363 293 205 180 155 148 105 75
63 18 9 9
NspI r_CATG'y
Cuts at: 0 507 1020 1389 1623
Size: 507 513 369 234
Fragments arranged by size:
513 507 369 234
PasI CC'CwG_GG
Cuts at: 0 1056 1581 1623
Size: 1056 525 42
Fragments arranged by size:
1056 525 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 129 186 841 865 1623
Size: 129 57 655 24 758
Fragments arranged by size:
758 655 129 57 24
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 514 546 1623
Size: 514 32 1077
Fragments arranged by size:
1077 514 32
PpuMI rG'GwC_Cy
Cuts at: 0 82 1623
Size: 82 1541
PspOMI G'GGCC_C
Cuts at: 0 89 421 688 1051 1059 1529 1569 1623
Size: 89 332 267 363 8 470 40 54
Fragments arranged by size:
470 363 332 267 89 54 40 8
PspXI vC'TCGA_Gb
Cuts at: 0 483 1623
Size: 483 1140
PstI C_TGCA'G
Cuts at: 0 646 1623
Size: 646 977
PvuII CAG'CTG
Cuts at: 0 363 873 1355 1623
Size: 363 510 482 268
Fragments arranged by size:
510 482 363 268
SacI G_AGCT'C
Cuts at: 0 1564 1623
Size: 1564 59
SexAI A'CCwGG_T
Cuts at: 0 837 1623
Size: 837 786
SfcI C'TryA_G
Cuts at: 0 642 1083 1350 1623
Size: 642 441 267 273
Fragments arranged by size:
642 441 273 267
SmaI CCC'GGG
Cuts at: 0 87 1623
Size: 87 1536
SmlI C'TyrA_G
Cuts at: 0 483 1291 1623
Size: 483 808 332
Fragments arranged by size:
808 483 332
SphI G_CATG'C
Cuts at: 0 1020 1623
Size: 1020 603
StuI AGG'CCT
Cuts at: 0 231 1623
Size: 231 1392
StyI C'CwwG_G
Cuts at: 0 937 1623
Size: 937 686
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 1623
Size: 610 1013
TatI w'GTAC_w
Cuts at: 0 446 730 1623
Size: 446 284 893
Fragments arranged by size:
893 446 284
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1095 1127 1623
Size: 1095 32 496
Fragments arranged by size:
1095 496 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1477 1623
Size: 1477 146
XhoI C'TCGA_G
Cuts at: 0 483 1623
Size: 483 1140
XmaI C'CCGG_G
Cuts at: 0 85 1623
Size: 85 1538
XmnI GAAnn'nnTTC
Cuts at: 0 529 1159 1411 1623
Size: 529 630 252 212
Fragments arranged by size:
630 529 252 212
Enzymes that do cut:
AarI AcuI AhdI AleI AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BciVI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII MscI MslI MspA1I NspI
PasI PflMI PpiI PpuMI PspOMI PspXI PstI PvuII
SacI SexAI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BclI BfrBI BglII BmgBI
BmtI BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI ZraI