Restriction map for
pFN21AE6447
(Linear) (Six-base) MAPSORT of: KIEE6447.seq Check: 5420 from: 1 to: 1518
KIEE6447.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
Acc65I G'GTAC_C
Cuts at: 0 184 1518
Size: 184 1334
AclI AA'CG_TT
Cuts at: 0 447 1518
Size: 447 1071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 373 1518
Size: 319 54 1145
Fragments arranged by size:
1145 319 54
AleI CACnn'nnGTG
Cuts at: 0 1364 1518
Size: 1364 154
AlwNI CAG_nnn'CTG
Cuts at: 0 57 888 1518
Size: 57 831 630
Fragments arranged by size:
831 630 57
ApaLI G'TGCA_C
Cuts at: 0 78 1518
Size: 78 1440
ApoI r'AATT_y
Cuts at: 0 159 227 1479 1493 1518
Size: 159 68 1252 14 25
Fragments arranged by size:
1252 159 68 25 14
AvaI C'yCGr_G
Cuts at: 0 977 1518
Size: 977 541
BamHI G'GATC_C
Cuts at: 0 858 1518
Size: 858 660
BanI G'GyrC_C
Cuts at: 0 184 1131 1518
Size: 184 947 387
Fragments arranged by size:
947 387 184
BanII G_rGCy'C
Cuts at: 0 341 484 1055 1518
Size: 341 143 571 463
Fragments arranged by size:
571 463 341 143
BbsI GAAGACnn'nnnn_
Cuts at: 0 1201 1518
Size: 1201 317
BbvCI CC'TCA_GC
Cuts at: 0 203 967 1518
Size: 203 764 551
Fragments arranged by size:
764 551 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 50 84 1518
Size: 50 34 1434
Fragments arranged by size:
1434 50 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 843 1518
Size: 843 675
BglII A'GATC_T
Cuts at: 0 953 1518
Size: 953 565
BlpI GC'TnA_GC
Cuts at: 0 46 1518
Size: 46 1472
Bme1580I G_kGCm'C
Cuts at: 0 82 1518
Size: 82 1436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 640 1518
Size: 640 878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1518
Size: 910 608
Bpu10I CC'TnA_GC
Cuts at: 0 203 967 1518
Size: 203 764 551
Fragments arranged by size:
764 551 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1214 1518
Size: 1214 304
BsaI GGTCTCn'nnnn_
Cuts at: 0 896 1518
Size: 896 622
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 699 1063 1471 1518
Size: 699 364 408 47
Fragments arranged by size:
699 408 364 47
BseYI C'CCAG_C
Cuts at: 0 285 1042 1352 1518
Size: 285 757 310 166
Fragments arranged by size:
757 310 285 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 1518
Size: 1342 176
BsiEI CG_ry'CG
Cuts at: 0 64 1518
Size: 64 1454
BsiHKAI G_wGCw'C
Cuts at: 0 82 1375 1518
Size: 82 1293 143
Fragments arranged by size:
1293 143 82
BsmI GAATG_Cn'
Cuts at: 0 1379 1518
Size: 1379 139
Bsp1286I G_dGCh'C
Cuts at: 0 82 341 484 1055 1375 1518
Size: 82 259 143 571 320 143
Fragments arranged by size:
571 320 259 143 143 82
BsrDI GCAATG_nn'
Cuts at: 0 533 1518
Size: 533 985
BsrFI r'CCGG_y
Cuts at: 0 641 1518
Size: 641 877
BstXI CCAn_nnnn'nTGG
Cuts at: 0 823 1518
Size: 823 695
BstYI r'GATC_y
Cuts at: 0 192 858 953 1433 1518
Size: 192 666 95 480 85
Fragments arranged by size:
666 480 192 95 85
Bsu36I CC'TnA_GG
Cuts at: 0 785 1518
Size: 785 733
BtsI GCAGTG_nn'
Cuts at: 0 125 1022 1074 1518
Size: 125 897 52 444
Fragments arranged by size:
897 444 125 52
DraI TTT'AAA
Cuts at: 0 255 1518
Size: 255 1263
DraIII CAC_nnn'GTG
Cuts at: 0 1131 1518
Size: 1131 387
EaeI y'GGCC_r
Cuts at: 0 419 1241 1445 1463 1518
Size: 419 822 204 18 55
Fragments arranged by size:
822 419 204 55 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 660 1518
Size: 660 858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 373 910 1518
Size: 319 54 537 608
Fragments arranged by size:
608 537 319 54
EcoO109I rG'GnC_Cy
Cuts at: 0 29 885 894 981 1518
Size: 29 856 9 87 537
Fragments arranged by size:
856 537 87 29 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 975 1518
Size: 975 543
HaeII r_GCGC'y
Cuts at: 0 1089 1518
Size: 1089 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 940 972 1020 1052 1518
Size: 361 32 547 32 48 32 466
Fragments arranged by size:
547 466 361 48 32 32 32
HincII GTy'rAC
Cuts at: 0 125 1518
Size: 125 1393
HindIII A'AGCT_T
Cuts at: 0 1279 1518
Size: 1279 239
HpaI GTT'AAC
Cuts at: 0 125 1518
Size: 125 1393
KpnI G_GTAC'C
Cuts at: 0 188 1518
Size: 188 1330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1518
Size: 438 1080
MscI TGG'CCA
Cuts at: 0 421 1243 1447 1465 1518
Size: 421 822 204 18 53
Fragments arranged by size:
822 421 204 53 18
MslI CAynn'nnrTG
Cuts at: 0 1364 1518
Size: 1364 154
MspA1I CmG'CkG
Cuts at: 0 248 1356 1518
Size: 248 1108 162
Fragments arranged by size:
1108 248 162
NdeI CA'TA_TG
Cuts at: 0 209 1518
Size: 209 1309
NspI r_CATG'y
Cuts at: 0 1110 1518
Size: 1110 408
PstI C_TGCA'G
Cuts at: 0 1028 1518
Size: 1028 490
PvuII CAG'CTG
Cuts at: 0 248 1356 1518
Size: 248 1108 162
Fragments arranged by size:
1108 248 162
RsrII CG'GwC_CG
Cuts at: 0 90 1518
Size: 90 1428
SfcI C'TryA_G
Cuts at: 0 1024 1220 1518
Size: 1024 196 298
Fragments arranged by size:
1024 298 196
SmlI C'TyrA_G
Cuts at: 0 1229 1518
Size: 1229 289
StuI AGG'CCT
Cuts at: 0 776 1518
Size: 776 742
StyI C'CwwG_G
Cuts at: 0 495 1118 1518
Size: 495 623 400
Fragments arranged by size:
623 495 400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1365 1397 1518
Size: 1365 32 121
Fragments arranged by size:
1365 121 32
Tth111I GACn'n_nGTC
Cuts at: 0 811 1518
Size: 811 707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 823 1518
Size: 823 695
Enzymes that do cut:
Acc65I AclI AcuI AleI AlwNI ApaLI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BglII
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrDI BsrFI
BstXI BstYI Bsu36I BtsI DraI DraIII EaeI EciI
Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII HpaI
KpnI MmeI MscI MslI MspA1I NdeI NspI PstI
PvuII RsrII SfcI SmlI StuI StyI TstI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BbeI BclI BfrBI BglI BmgBI BmrI BmtI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgI BtgZI ClaI CspCI DrdI EagI EarI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI KasI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI XbaI XhoI
XmaI XmnI ZraI