(Linear) (Six-base) MAPSORT of: KIEE6445.seq  Check: 394  from: 1  to: 1563



KIEE6445.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:31  ..

AccI GT'mk_AC

Cuts at:      0     404     482    1563 
   Size:        404      78    1081

  Fragments arranged by size: 

                1081     404      78
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583     683    1055    1563 
   Size:        583     100     372     508

  Fragments arranged by size: 

                 583     508     372     100
 
AflIII A'CryG_T

Cuts at:      0     654    1563 
   Size:        654     909
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     298    1563 
   Size:        298    1265
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1482    1514    1563 
   Size:       1482      32      49

  Fragments arranged by size: 

                1482      49      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     606    1342    1563 
   Size:        606     736     221

  Fragments arranged by size: 

                 736     606     221
 
ApaLI G'TGCA_C

Cuts at:      0     449    1563 
   Size:        449    1114
 
ApoI r'AATT_y

Cuts at:      0      87     184     614    1563 
   Size:         87      97     430     949

  Fragments arranged by size: 

                 949     430      97      87
 
BanII G_rGCy'C

Cuts at:      0     801    1239    1563 
   Size:        801     438     324

  Fragments arranged by size: 

                 801     438     324
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     680    1563 
   Size:        680     883
 
BfrBI ATG'CAT

Cuts at:      0     299    1563 
   Size:        299    1264
 
Bme1580I G_kGCm'C

Cuts at:      0     453    1563 
   Size:        453    1110
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     581    1563 
   Size:        581     982
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1278    1563 
   Size:       1278     285
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1187    1563 
   Size:       1187     376
 
Bpu10I CC'TnA_GC

Cuts at:      0    1304    1563 
   Size:       1304     259
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1273    1563 
   Size:       1273     290
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1521    1563 
   Size:       1521      42
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      19      49    1563 
   Size:         19      30    1514

  Fragments arranged by size: 

                1514      30      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     217    1563 
   Size:        217    1346
 
BseYI C'CCAG_C

Cuts at:      0     458     800    1420    1563 
   Size:        458     342     620     143

  Fragments arranged by size: 

                 620     458     342     143
 
BsiHKAI G_wGCw'C

Cuts at:      0     453    1563 
   Size:        453    1110
 
Bsp1286I G_dGCh'C

Cuts at:      0     453     801    1239    1563 
   Size:        453     348     438     324

  Fragments arranged by size: 

                 453     438     348     324
 
BsrDI GCAATG_nn'

Cuts at:      0     823     853    1563 
   Size:        823      30     710

  Fragments arranged by size: 

                 823     710      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     296    1563 
   Size:        296    1267
 
BstYI r'GATC_y

Cuts at:      0     759    1563 
   Size:        759     804
 
BstZ17I GTA'TAC

Cuts at:      0     405     483    1563 
   Size:        405      78    1080

  Fragments arranged by size: 

                1080     405      78
 
BtgI C'CryG_G

Cuts at:      0     431    1317    1563 
   Size:        431     886     246

  Fragments arranged by size: 

                 886     431     246
 
BtsI GCAGTG_nn'

Cuts at:      0     201     297    1563 
   Size:        201      96    1266

  Fragments arranged by size: 

                1266     201      96
 
DraIII CAC_nnn'GTG

Cuts at:      0     148    1563 
   Size:        148    1415
 
EarI CTCTTCn'nnn_

Cuts at:      0     314     602    1264    1563 
   Size:        314     288     662     299

  Fragments arranged by size: 

                 662     314     299     288
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583     683    1055    1187    1563 
   Size:        583     100     372     132     376

  Fragments arranged by size: 

                 583     376     372     132     100
 
EcoRI G'AATT_C

Cuts at:      0     184    1563 
   Size:        184    1379
 
EcoRV GAT'ATC

Cuts at:      0     643    1031    1563 
   Size:        643     388     532

  Fragments arranged by size: 

                 643     532     388
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     328    1486    1563 
   Size:        328    1158      77

  Fragments arranged by size: 

                1158     328      77
 
HincII GTy'rAC

Cuts at:      0     342     542     722    1041    1563 
   Size:        342     200     180     319     522

  Fragments arranged by size: 

                 522     342     319     200     180
 
HindIII A'AGCT_T

Cuts at:      0    1468    1563 
   Size:       1468      95
 
HpaI GTT'AAC

Cuts at:      0     722    1563 
   Size:        722     841
 
MfeI C'AATT_G

Cuts at:      0    1107    1563 
   Size:       1107     456
 
MslI CAynn'nnrTG

Cuts at:      0     290    1563 
   Size:        290    1273
 
NcoI C'CATG_G

Cuts at:      0     431    1317    1563 
   Size:        431     886     246

  Fragments arranged by size: 

                 886     431     246
 
NsiI A_TGCA'T

Cuts at:      0     301    1563 
   Size:        301    1262
 
NspI r_CATG'y

Cuts at:      0     585     658     960    1563 
   Size:        585      73     302     603

  Fragments arranged by size: 

                 603     585     302      73
 
PciI A'CATG_T

Cuts at:      0     654    1563 
   Size:        654     909
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1323    1563 
   Size:       1323     240
 
PfoI T'CCnGG_A

Cuts at:      0     134    1563 
   Size:        134    1429
 
PsiI TTA'TAA

Cuts at:      0     746    1563 
   Size:        746     817
 
SapI GCTCTTCn'nnn_

Cuts at:      0     314    1563 
   Size:        314    1249
 
SfcI C'TryA_G

Cuts at:      0    1187    1563 
   Size:       1187     376
 
SmlI C'TyrA_G

Cuts at:      0    1252    1563 
   Size:       1252     311
 
StyI C'CwwG_G

Cuts at:      0     431    1317    1563 
   Size:        431     886     246

  Fragments arranged by size: 

                 886     431     246
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     695     727    1563 
   Size:        695      32     836

  Fragments arranged by size: 

                 836     695      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     245    1563 
   Size:        245    1318
 
XmnI GAAnn'nnTTC

Cuts at:      0     308    1563 
   Size:        308    1255
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI     AloI    AlwNI    ApaLI     ApoI
    BanII    BciVI    BfrBI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BsrDI
   BstAPI    BstYI  BstZ17I     BtgI     BtsI   DraIII     EarI  Eco57MI
    EcoRI    EcoRV     FalI   HincII  HindIII     HpaI     MfeI     MslI
     NcoI     NsiI     NspI     PciI    PflMI     PfoI     PsiI     SapI
     SfcI     SmlI     StyI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI     BclI     BglI
    BglII     BlpI    BmgBI     BmtI    BsaAI    BsaBI    BsaHI    BsaWI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I     FseI     FspI    FspAI    HaeII
    Hin4I     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     ZraI