Restriction map for
pFN21AE6442
(Linear) (Six-base) MAPSORT of: KIEE6442.seq Check: 7723 from: 1 to: 1365
KIEE6442.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 833 1365
Size: 833 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1232 1365
Size: 1232 133
AfeI AGC'GCT
Cuts at: 0 898 1365
Size: 898 467
AleI CACnn'nnGTG
Cuts at: 0 161 1365
Size: 161 1204
AlwNI CAG_nnn'CTG
Cuts at: 0 303 1332 1365
Size: 303 1029 33
Fragments arranged by size:
1029 303 33
ApaI G_GGCC'C
Cuts at: 0 574 1026 1235 1272 1365
Size: 574 452 209 37 93
Fragments arranged by size:
574 452 209 93 37
AvaI C'yCGr_G
Cuts at: 0 139 858 1365
Size: 139 719 507
Fragments arranged by size:
719 507 139
AvrII C'CTAG_G
Cuts at: 0 1206 1365
Size: 1206 159
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 237 270 1365
Size: 237 33 1095
Fragments arranged by size:
1095 237 33
BamHI G'GATC_C
Cuts at: 0 986 1365
Size: 986 379
BanI G'GyrC_C
Cuts at: 0 347 606 693 970 1038 1128 1365
Size: 347 259 87 277 68 90 237
Fragments arranged by size:
347 277 259 237 90 87 68
BanII G_rGCy'C
Cuts at: 0 505 574 1026 1235 1272 1301 1365
Size: 505 69 452 209 37 29 64
Fragments arranged by size:
505 452 209 69 64 37 29
BbeI G_GCGC'C
Cuts at: 0 351 697 1365
Size: 351 346 668
Fragments arranged by size:
668 351 346
BglI GCCn_nnn'nGGC
Cuts at: 0 430 824 1365
Size: 430 394 541
Fragments arranged by size:
541 430 394
BlpI GC'TnA_GC
Cuts at: 0 736 805 877 1365
Size: 736 69 72 488
Fragments arranged by size:
736 488 72 69
Bme1580I G_kGCm'C
Cuts at: 0 168 574 1026 1043 1133 1183 1235 1272
Size: 168 406 452 17 90 50 52 37
Cuts at: 1272 1365
Size: 93
Fragments arranged by size:
452 406 168 93 90 52 50 37
17
BmrI ACTGGGnnnn_n'
Cuts at: 0 329 565 1365
Size: 329 236 800
Fragments arranged by size:
800 329 236
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 820 1365
Size: 820 545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 703 815 886 1365
Size: 420 283 112 71 479
Fragments arranged by size:
479 420 283 112 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 1365
Size: 1339 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 274 1365
Size: 274 1091
BsaAI yAC'GTr
Cuts at: 0 1289 1365
Size: 1289 76
BsaHI Gr'CG_yC
Cuts at: 0 348 694 1365
Size: 348 346 671
Fragments arranged by size:
671 348 346
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 1365
Size: 229 30 1106
Fragments arranged by size:
1106 229 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1042 1365
Size: 1042 323
BseYI C'CCAG_C
Cuts at: 0 1061 1083 1365
Size: 1061 22 282
Fragments arranged by size:
1061 282 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 1365
Size: 9 1356
BsiEI CG_ry'CG
Cuts at: 0 162 1365
Size: 162 1203
BsiHKAI G_wGCw'C
Cuts at: 0 1215 1365
Size: 1215 150
BsmI GAATG_Cn'
Cuts at: 0 283 1365
Size: 283 1082
BsmBI CGTCTCn'nnnn_
Cuts at: 0 664 1365
Size: 664 701
Bsp1286I G_dGCh'C
Cuts at: 0 168 505 574 1026 1043 1133 1183 1215
Size: 168 337 69 452 17 90 50 32
Cuts at: 1215 1235 1272 1301 1365
Size: 20 37 29 64
Fragments arranged by size:
452 337 168 90 69 64 50 37
32 29 20 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 650 1365
Size: 650 715
BssSI C'ACGA_G
Cuts at: 0 773 1365
Size: 773 592
BstEII G'GTnAC_C
Cuts at: 0 72 1365
Size: 72 1293
BstYI r'GATC_y
Cuts at: 0 986 1365
Size: 986 379
BtgI C'CryG_G
Cuts at: 0 156 479 953 1145 1226 1365
Size: 156 323 474 192 81 139
Fragments arranged by size:
474 323 192 156 139 81
BtsI GCAGTG_nn'
Cuts at: 0 1195 1365
Size: 1195 170
DraIII CAC_nnn'GTG
Cuts at: 0 1289 1365
Size: 1289 76
EaeI y'GGCC_r
Cuts at: 0 159 380 482 777 956 1217 1241 1262
Size: 159 221 102 295 179 261 24 21
Cuts at: 1262 1365
Size: 103
Fragments arranged by size:
295 261 221 179 159 103 102 24
21
EagI C'GGCC_G
Cuts at: 0 159 1365
Size: 159 1206
EarI CTCTTCn'nnn_
Cuts at: 0 235 631 853 1365
Size: 235 396 222 512
Fragments arranged by size:
512 396 235 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 999 1365
Size: 999 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 703 815 886 1232 1365
Size: 420 283 112 71 346 133
Fragments arranged by size:
420 346 283 133 112 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 919 1365
Size: 919 446
EcoO109I rG'GnC_Cy
Cuts at: 0 571 783 1023 1231 1232 1268 1365
Size: 571 212 240 208 1 36 97
Fragments arranged by size:
571 240 212 208 97 36 1
FspI TGC'GCA
Cuts at: 0 1201 1365
Size: 1201 164
HaeII r_GCGC'y
Cuts at: 0 66 351 697 900 1365
Size: 66 285 346 203 465
Fragments arranged by size:
465 346 285 203 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 775 807 1365
Size: 487 32 256 32 558
Fragments arranged by size:
558 487 256 32 32
KasI G'GCGC_C
Cuts at: 0 347 693 1365
Size: 347 346 672
Fragments arranged by size:
672 347 346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 662 1365
Size: 128 534 703
Fragments arranged by size:
703 534 128
MscI TGG'CCA
Cuts at: 0 382 779 958 1219 1365
Size: 382 397 179 261 146
Fragments arranged by size:
397 382 261 179 146
MslI CAynn'nnrTG
Cuts at: 0 161 226 541 1186 1365
Size: 161 65 315 645 179
Fragments arranged by size:
645 315 179 161 65
MspA1I CmG'CkG
Cuts at: 0 1228 1365
Size: 1228 137
NarI GG'CG_CC
Cuts at: 0 348 694 1365
Size: 348 346 671
Fragments arranged by size:
671 348 346
NcoI C'CATG_G
Cuts at: 0 953 1365
Size: 953 412
NdeI CA'TA_TG
Cuts at: 0 390 1365
Size: 390 975
PasI CC'CwG_GG
Cuts at: 0 567 708 1365
Size: 567 141 657
Fragments arranged by size:
657 567 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 319 793 1365
Size: 319 474 572
Fragments arranged by size:
572 474 319
PmlI CAC'GTG
Cuts at: 0 1289 1365
Size: 1289 76
PpuMI rG'GwC_Cy
Cuts at: 0 783 1365
Size: 783 582
PspOMI G'GGCC_C
Cuts at: 0 570 1022 1231 1268 1365
Size: 570 452 209 37 97
Fragments arranged by size:
570 452 209 97 37
PstI C_TGCA'G
Cuts at: 0 299 1328 1365
Size: 299 1029 37
Fragments arranged by size:
1029 299 37
SacII CC_GC'GG
Cuts at: 0 1229 1365
Size: 1229 136
SapI GCTCTTCn'nnn_
Cuts at: 0 631 1365
Size: 631 734
SfcI C'TryA_G
Cuts at: 0 295 916 1324 1365
Size: 295 621 408 41
Fragments arranged by size:
621 408 295 41
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 430 1365
Size: 430 935
SfoI GGC'GCC
Cuts at: 0 349 695 1365
Size: 349 346 670
Fragments arranged by size:
670 349 346
SmaI CCC'GGG
Cuts at: 0 141 1365
Size: 141 1224
SmlI C'TyrA_G
Cuts at: 0 1354 1365
Size: 1354 11
SrfI GCCC'GGGC
Cuts at: 0 141 1365
Size: 141 1224
StuI AGG'CCT
Cuts at: 0 1276 1365
Size: 1276 89
StyI C'CwwG_G
Cuts at: 0 953 1206 1365
Size: 953 253 159
Fragments arranged by size:
953 253 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 54 364 1365
Size: 54 310 1001
Fragments arranged by size:
1001 310 54
TatI w'GTAC_w
Cuts at: 0 869 1253 1365
Size: 869 384 112
Fragments arranged by size:
869 384 112
XmaI C'CCGG_G
Cuts at: 0 139 1365
Size: 139 1226
Enzymes that do cut:
AccI AcuI AfeI AleI AlwNI ApaI AvaI AvrII
BaeI BamHI BanI BanII BbeI BglI BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BssSI BstEII BstYI BtgI BtsI DraIII EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI
NdeI PasI PflMI PmlI PpuMI PspOMI PstI SacII
SapI SfcI SfiI SfoI SmaI SmlI SrfI StuI
StyI TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
Bpu10I BsaBI BsaWI BsiWI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI NaeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI