Restriction map for
pFN21AE6433
(Linear) (Six-base) MAPSORT of: KIEE6433.seq Check: 623 from: 1 to: 1341
KIEE6433.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 510 1341
Size: 510 831
AccI GT'mk_AC
Cuts at: 0 469 1158 1341
Size: 469 689 183
Fragments arranged by size:
689 469 183
Acc65I G'GTAC_C
Cuts at: 0 269 1341
Size: 269 1072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1341
Size: 853 488
AflIII A'CryG_T
Cuts at: 0 206 1214 1341
Size: 206 1008 127
Fragments arranged by size:
1008 206 127
AgeI A'CCGG_T
Cuts at: 0 272 1341
Size: 272 1069
AhdI GACnn_n'nnGTC
Cuts at: 0 465 1341
Size: 465 876
AlwNI CAG_nnn'CTG
Cuts at: 0 309 990 1341
Size: 309 681 351
Fragments arranged by size:
681 351 309
ApaI G_GGCC'C
Cuts at: 0 342 1341
Size: 342 999
ApaLI G'TGCA_C
Cuts at: 0 999 1341
Size: 999 342
ApoI r'AATT_y
Cuts at: 0 501 676 737 1078 1103 1341
Size: 501 175 61 341 25 238
Fragments arranged by size:
501 341 238 175 61 25
AvaI C'yCGr_G
Cuts at: 0 244 344 1341
Size: 244 100 997
Fragments arranged by size:
997 244 100
AvrII C'CTAG_G
Cuts at: 0 1108 1341
Size: 1108 233
BanI G'GyrC_C
Cuts at: 0 145 269 331 1341
Size: 145 124 62 1010
Fragments arranged by size:
1010 145 124 62
BanII G_rGCy'C
Cuts at: 0 342 1341
Size: 342 999
BbsI GAAGACnn'nnnn_
Cuts at: 0 804 1063 1338 1341
Size: 804 259 275 3
Fragments arranged by size:
804 275 259 3
BciVI GTATCCnnnnn_n'
Cuts at: 0 653 1341
Size: 653 688
BclI T'GATC_A
Cuts at: 0 300 1341
Size: 300 1041
BfrBI ATG'CAT
Cuts at: 0 1140 1341
Size: 1140 201
Bme1580I G_kGCm'C
Cuts at: 0 342 850 1003 1179 1341
Size: 342 508 153 176 162
Fragments arranged by size:
508 342 176 162 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 1044 1341
Size: 1044 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 1145 1341
Size: 93 1052 196
Fragments arranged by size:
1052 196 93
BsaI GGTCTCn'nnnn_
Cuts at: 0 204 587 1341
Size: 204 383 754
Fragments arranged by size:
754 383 204
BsaHI Gr'CG_yC
Cuts at: 0 128 1341
Size: 128 1213
BsaWI w'CCGG_w
Cuts at: 0 272 510 1341
Size: 272 238 831
Fragments arranged by size:
831 272 238
BseYI C'CCAG_C
Cuts at: 0 239 1341
Size: 239 1102
BsiEI CG_ry'CG
Cuts at: 0 156 473 1341
Size: 156 317 868
Fragments arranged by size:
868 317 156
BsiHKAI G_wGCw'C
Cuts at: 0 950 1003 1341
Size: 950 53 338
Fragments arranged by size:
950 338 53
BsmI GAATG_Cn'
Cuts at: 0 539 612 981 1341
Size: 539 73 369 360
Fragments arranged by size:
539 369 360 73
Bsp1286I G_dGCh'C
Cuts at: 0 342 850 950 1003 1179 1341
Size: 342 508 100 53 176 162
Fragments arranged by size:
508 342 176 162 100 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 510 605 1341
Size: 510 95 736
Fragments arranged by size:
736 510 95
BsrDI GCAATG_nn'
Cuts at: 0 87 1341
Size: 87 1254
BsrFI r'CCGG_y
Cuts at: 0 148 272 377 1341
Size: 148 124 105 964
Fragments arranged by size:
964 148 124 105
BssSI C'ACGA_G
Cuts at: 0 43 1341
Size: 43 1298
BstBI TT'CG_AA
Cuts at: 0 414 1341
Size: 414 927
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1082 1341
Size: 1082 259
BstYI r'GATC_y
Cuts at: 0 1112 1341
Size: 1112 229
Bsu36I CC'TnA_GG
Cuts at: 0 310 1341
Size: 310 1031
BtgI C'CryG_G
Cuts at: 0 156 1341
Size: 156 1185
EaeI y'GGCC_r
Cuts at: 0 50 153 657 1341
Size: 50 103 504 684
Fragments arranged by size:
684 504 103 50
EagI C'GGCC_G
Cuts at: 0 153 1341
Size: 153 1188
EarI CTCTTCn'nnn_
Cuts at: 0 770 1052 1341
Size: 770 282 289
Fragments arranged by size:
770 289 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 265 1341
Size: 265 1076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 853 1145 1341
Size: 93 760 292 196
Fragments arranged by size:
760 292 196 93
EcoNI CCTnn'n_nnAGG
Cuts at: 0 775 1341
Size: 775 566
EcoO109I rG'GnC_Cy
Cuts at: 0 105 339 875 1341
Size: 105 234 536 466
Fragments arranged by size:
536 466 234 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 451 483 678 710 850 882
Size: 157 32 262 32 195 32 140 32
Cuts at: 882 1341
Size: 459
Fragments arranged by size:
459 262 195 157 140 32 32 32
32
HincII GTy'rAC
Cuts at: 0 470 1341
Size: 470 871
KpnI G_GTAC'C
Cuts at: 0 273 1341
Size: 273 1068
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1341
Size: 227 1114
MspA1I CmG'CkG
Cuts at: 0 158 291 486 1341
Size: 158 133 195 855
Fragments arranged by size:
855 195 158 133
NaeI GCC'GGC
Cuts at: 0 150 1341
Size: 150 1191
NgoMIV G'CCGG_C
Cuts at: 0 148 1341
Size: 148 1193
NotI GC'GGCC_GC
Cuts at: 0 153 1341
Size: 153 1188
NsiI A_TGCA'T
Cuts at: 0 1142 1341
Size: 1142 199
NspI r_CATG'y
Cuts at: 0 1218 1341
Size: 1218 123
PasI CC'CwG_GG
Cuts at: 0 423 870 1341
Size: 423 447 471
Fragments arranged by size:
471 447 423
PciI A'CATG_T
Cuts at: 0 1214 1341
Size: 1214 127
PpuMI rG'GwC_Cy
Cuts at: 0 875 1341
Size: 875 466
PsiI TTA'TAA
Cuts at: 0 1031 1341
Size: 1031 310
PspOMI G'GGCC_C
Cuts at: 0 338 1341
Size: 338 1003
PstI C_TGCA'G
Cuts at: 0 521 1340 1341
Size: 521 819 1
Fragments arranged by size:
819 521 1
PvuII CAG'CTG
Cuts at: 0 291 486 1341
Size: 291 195 855
Fragments arranged by size:
855 291 195
SacII CC_GC'GG
Cuts at: 0 159 1341
Size: 159 1182
SalI G'TCGA_C
Cuts at: 0 468 1341
Size: 468 873
SfcI C'TryA_G
Cuts at: 0 517 879 1155 1336 1341
Size: 517 362 276 181 5
Fragments arranged by size:
517 362 276 181 5
SmaI CCC'GGG
Cuts at: 0 346 1341
Size: 346 995
StyI C'CwwG_G
Cuts at: 0 1108 1341
Size: 1108 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 487 1341
Size: 487 854
TatI w'GTAC_w
Cuts at: 0 1015 1341
Size: 1015 326
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 867 899 1341
Size: 867 32 442
Fragments arranged by size:
867 442 32
Tth111I GACn'n_nGTC
Cuts at: 0 436 1341
Size: 436 905
XmaI C'CCGG_G
Cuts at: 0 344 1341
Size: 344 997
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AvaI AvrII BanI BanII BbsI
BciVI BclI BfrBI Bme1580I BmrI BpmI BsaI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrFI BssSI BstBI BstXI BstYI Bsu36I BtgI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I Hin4I HincII
KpnI MmeI MspA1I NaeI NgoMIV NotI NsiI NspI
PasI PciI PpuMI PsiI PspOMI PstI PvuII SacII
SalI SfcI SmaI StyI TaqII TatI TstI Tth111I
XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AleI AlfI AloI AscI
AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaXI BseRI BsgI BsiWI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BstAPI BstEII BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
MfeI MluI MscI MslI NarI NcoI NdeI NheI
NruI PacI PflMI PfoI PmeI PmlI PpiI PshAI
PspXI PsrI PvuI RsrII SacI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XcmI
XhoI XmnI ZraI