Restriction map for
pFN21AE6429
(Linear) (Six-base) MAPSORT of: KIEE6429.seq Check: 2358 from: 1 to: 1785
KIEE6429.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 711 1785
Size: 711 1074
AccI GT'mk_AC
Cuts at: 0 871 1785
Size: 871 914
AflIII A'CryG_T
Cuts at: 0 313 1785
Size: 313 1472
AhdI GACnn_n'nnGTC
Cuts at: 0 867 1785
Size: 867 918
AlwNI CAG_nnn'CTG
Cuts at: 0 38 1785
Size: 38 1747
ApaI G_GGCC'C
Cuts at: 0 173 1785
Size: 173 1612
ApoI r'AATT_y
Cuts at: 0 255 1785
Size: 255 1530
AvaI C'yCGr_G
Cuts at: 0 6 1785
Size: 6 1779
AvrII C'CTAG_G
Cuts at: 0 1671 1785
Size: 1671 114
BanI G'GyrC_C
Cuts at: 0 542 1785
Size: 542 1243
BanII G_rGCy'C
Cuts at: 0 7 173 1785
Size: 7 166 1612
Fragments arranged by size:
1612 166 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 893 1754 1785
Size: 893 861 31
Fragments arranged by size:
893 861 31
BclI T'GATC_A
Cuts at: 0 534 1785
Size: 534 1251
BglII A'GATC_T
Cuts at: 0 1421 1785
Size: 1421 364
Bme1580I G_kGCm'C
Cuts at: 0 173 321 1785
Size: 173 148 1464
Fragments arranged by size:
1464 173 148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1587 1785
Size: 1587 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1785
Size: 110 1675
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1164 1419 1785
Size: 1096 68 255 366
Fragments arranged by size:
1096 366 255 68
BsaBI GATnn'nnATC
Cuts at: 0 812 1785
Size: 812 973
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1597 1627 1785
Size: 1597 30 158
Fragments arranged by size:
1597 158 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 234 1033 1785
Size: 234 799 752
Fragments arranged by size:
799 752 234
BseYI C'CCAG_C
Cuts at: 0 47 80 116 1785
Size: 47 33 36 1669
Fragments arranged by size:
1669 47 36 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 587 1785
Size: 208 379 1198
Fragments arranged by size:
1198 379 208
BsiHKAI G_wGCw'C
Cuts at: 0 7 224 1785
Size: 7 217 1561
Fragments arranged by size:
1561 217 7
BsmI GAATG_Cn'
Cuts at: 0 1316 1785
Size: 1316 469
BsmBI CGTCTCn'nnnn_
Cuts at: 0 16 1785
Size: 16 1769
Bsp1286I G_dGCh'C
Cuts at: 0 7 173 224 321 1785
Size: 7 166 51 97 1464
Fragments arranged by size:
1464 166 97 51 7
BspHI T'CATG_A
Cuts at: 0 1509 1785
Size: 1509 276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 711 1305 1785
Size: 711 594 480
Fragments arranged by size:
711 594 480
BstYI r'GATC_y
Cuts at: 0 807 1421 1785
Size: 807 614 364
Fragments arranged by size:
807 614 364
Bsu36I CC'TnA_GG
Cuts at: 0 551 1785
Size: 551 1234
BtsI GCAGTG_nn'
Cuts at: 0 76 90 816 1785
Size: 76 14 726 969
Fragments arranged by size:
969 726 76 14
DraI TTT'AAA
Cuts at: 0 302 1785
Size: 302 1483
EarI CTCTTCn'nnn_
Cuts at: 0 1226 1526 1785
Size: 1226 300 259
Fragments arranged by size:
1226 300 259
EcoICRI GAG'CTC
Cuts at: 0 5 1785
Size: 5 1780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1785
Size: 110 1675
EcoO109I rG'GnC_Cy
Cuts at: 0 158 1436 1785
Size: 158 1278 349
Fragments arranged by size:
1278 349 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 853 880 885 912 1033 1065 1647 1679
Size: 853 27 5 27 121 32 582 32
Cuts at: 1679 1785
Size: 106
Fragments arranged by size:
853 582 121 106 32 32 27 27
5
HindIII A'AGCT_T
Cuts at: 0 1504 1521 1785
Size: 1504 17 264
Fragments arranged by size:
1504 264 17
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 394 603 863 1008 1785
Size: 319 75 209 260 145 777
Fragments arranged by size:
777 319 260 209 145 75
MslI CAynn'nnrTG
Cuts at: 0 1384 1460 1785
Size: 1384 76 325
Fragments arranged by size:
1384 325 76
MspA1I CmG'CkG
Cuts at: 0 38 1785
Size: 38 1747
NspI r_CATG'y
Cuts at: 0 317 1785
Size: 317 1468
PciI A'CATG_T
Cuts at: 0 313 1785
Size: 313 1472
PflMI CCAn_nnn'nTGG
Cuts at: 0 258 1200 1785
Size: 258 942 585
Fragments arranged by size:
942 585 258
PpuMI rG'GwC_Cy
Cuts at: 0 158 1436 1785
Size: 158 1278 349
Fragments arranged by size:
1278 349 158
PshAI GACnn'nnGTC
Cuts at: 0 1434 1785
Size: 1434 351
PspOMI G'GGCC_C
Cuts at: 0 169 1785
Size: 169 1616
PspXI vC'TCGA_Gb
Cuts at: 0 6 1785
Size: 6 1779
PstI C_TGCA'G
Cuts at: 0 71 956 1429 1785
Size: 71 885 473 356
Fragments arranged by size:
885 473 356 71
PvuII CAG'CTG
Cuts at: 0 38 1785
Size: 38 1747
SacI G_AGCT'C
Cuts at: 0 7 1785
Size: 7 1778
ScaI AGT'ACT
Cuts at: 0 327 1785
Size: 327 1458
SexAI A'CCwGG_T
Cuts at: 0 1707 1785
Size: 1707 78
SfcI C'TryA_G
Cuts at: 0 67 162 872 952 1425 1785
Size: 67 95 710 80 473 360
Fragments arranged by size:
710 473 360 95 80 67
SmlI C'TyrA_G
Cuts at: 0 6 1075 1179 1398 1785
Size: 6 1069 104 219 387
Fragments arranged by size:
1069 387 219 104 6
SpeI A'CTAG_T
Cuts at: 0 987 1785
Size: 987 798
SspI AAT'ATT
Cuts at: 0 1270 1785
Size: 1270 515
StuI AGG'CCT
Cuts at: 0 193 1676 1785
Size: 193 1483 109
Fragments arranged by size:
1483 193 109
StyI C'CwwG_G
Cuts at: 0 194 1671 1785
Size: 194 1477 114
Fragments arranged by size:
1477 194 114
TatI w'GTAC_w
Cuts at: 0 325 420 1649 1785
Size: 325 95 1229 136
Fragments arranged by size:
1229 325 136 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 150 182 1785
Size: 150 32 1603
Fragments arranged by size:
1603 150 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 1615 1785
Size: 180 1435 170
Fragments arranged by size:
1435 180 170
XhoI C'TCGA_G
Cuts at: 0 6 1785
Size: 6 1779
Enzymes that do cut:
AarI AccI AflIII AhdI AlwNI ApaI ApoI AvaI
AvrII BanI BanII BbsI BclI BglII Bme1580I BplI
BpmI BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BstYI Bsu36I BtsI
DraI EarI EcoICRI Eco57MI EcoO109I Hin4I HindIII MmeI
MslI MspA1I NspI PciI PflMI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI ScaI SexAI SfcI SmlI
SpeI SspI StuI StyI TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI
BmrI BmtI Bpu10I BsaI BsaAI BsaHI BsaWI BsiEI
BsiWI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PsiI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI TaqII Tth111I
XbaI XmaI XmnI ZraI