Restriction map for
pFN21AE6428
(Linear) (Six-base) MAPSORT of: KIEE6428.seq Check: 7096 from: 1 to: 1380
KIEE6428.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 575 1380
Size: 575 805
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 251 976 1380
Size: 69 182 725 404
Fragments arranged by size:
725 404 182 69
AhdI GACnn_n'nnGTC
Cuts at: 0 668 1380
Size: 668 712
AleI CACnn'nnGTG
Cuts at: 0 335 1380
Size: 335 1045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1140 1380
Size: 1140 240
AlwNI CAG_nnn'CTG
Cuts at: 0 601 1380
Size: 601 779
ApaI G_GGCC'C
Cuts at: 0 884 1380
Size: 884 496
ApoI r'AATT_y
Cuts at: 0 286 1380
Size: 286 1094
AseI AT'TA_AT
Cuts at: 0 416 1380
Size: 416 964
AvaI C'yCGr_G
Cuts at: 0 6 1380
Size: 6 1374
BanII G_rGCy'C
Cuts at: 0 29 884 1380
Size: 29 855 496
Fragments arranged by size:
855 496 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1149 1183 1380
Size: 1149 34 197
Fragments arranged by size:
1149 197 34
BglII A'GATC_T
Cuts at: 0 236 1380
Size: 236 1144
BlpI GC'TnA_GC
Cuts at: 0 100 1380
Size: 100 1280
Bme1580I G_kGCm'C
Cuts at: 0 884 1380
Size: 884 496
BmtI G_CTAG'C
Cuts at: 0 82 1141 1380
Size: 82 1059 239
Fragments arranged by size:
1059 239 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 175 1217 1380
Size: 127 48 1042 163
Fragments arranged by size:
1042 163 127 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1380
Size: 698 682
BsaHI Gr'CG_yC
Cuts at: 0 185 1380
Size: 185 1195
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1040 1070 1380
Size: 1040 30 310
Fragments arranged by size:
1040 310 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 71 421 1042 1380
Size: 71 350 621 338
Fragments arranged by size:
621 350 338 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 960 1380
Size: 190 770 420
Fragments arranged by size:
770 420 190
BsiEI CG_ry'CG
Cuts at: 0 44 1380
Size: 44 1336
BsiHKAI G_wGCw'C
Cuts at: 0 1084 1380
Size: 1084 296
BsmI GAATG_Cn'
Cuts at: 0 647 1314 1380
Size: 647 667 66
Fragments arranged by size:
667 647 66
Bsp1286I G_dGCh'C
Cuts at: 0 29 884 1084 1380
Size: 29 855 200 296
Fragments arranged by size:
855 296 200 29
BspHI T'CATG_A
Cuts at: 0 1298 1380
Size: 1298 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 753 1380
Size: 753 627
BsrFI r'CCGG_y
Cuts at: 0 46 1380
Size: 46 1334
BssSI C'ACGA_G
Cuts at: 0 1039 1380
Size: 1039 341
BstAPI GCAn_nnn'nTGC
Cuts at: 0 745 778 1380
Size: 745 33 602
Fragments arranged by size:
745 602 33
BstYI r'GATC_y
Cuts at: 0 236 1380
Size: 236 1144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 944 1380
Size: 944 436
ClaI AT'CG_AT
Cuts at: 0 950 1380
Size: 950 430
EaeI y'GGCC_r
Cuts at: 0 20 41 198 1380
Size: 20 21 157 1182
Fragments arranged by size:
1182 157 21 20
EagI C'GGCC_G
Cuts at: 0 41 1380
Size: 41 1339
EarI CTCTTCn'nnn_
Cuts at: 0 227 1380
Size: 227 1153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 79 1380
Size: 79 1301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 127 175 251 976 1217 1380
Size: 69 58 48 76 725 241 163
Fragments arranged by size:
725 241 163 76 69 58 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1024 1380
Size: 1024 356
EcoO109I rG'GnC_Cy
Cuts at: 0 880 1380
Size: 880 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 402 432 434 464 654 686 1068 1100
Size: 402 30 2 30 190 32 382 32
Cuts at: 1100 1380
Size: 280
Fragments arranged by size:
402 382 280 190 32 32 30 30
2
HincII GTy'rAC
Cuts at: 0 576 1322 1380
Size: 576 746 58
Fragments arranged by size:
746 576 58
HpaI GTT'AAC
Cuts at: 0 1322 1380
Size: 1322 58
MfeI C'AATT_G
Cuts at: 0 319 1380
Size: 319 1061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1380
Size: 561 819
MscI TGG'CCA
Cuts at: 0 200 1380
Size: 200 1180
MslI CAynn'nnrTG
Cuts at: 0 335 1153 1380
Size: 335 818 227
Fragments arranged by size:
818 335 227
MspA1I CmG'CkG
Cuts at: 0 204 1380
Size: 204 1176
NaeI GCC'GGC
Cuts at: 0 48 1380
Size: 48 1332
NdeI CA'TA_TG
Cuts at: 0 666 1380
Size: 666 714
NgoMIV G'CCGG_C
Cuts at: 0 46 1380
Size: 46 1334
NheI G'CTAG_C
Cuts at: 0 78 1137 1380
Size: 78 1059 243
Fragments arranged by size:
1059 243 78
NotI GC'GGCC_GC
Cuts at: 0 41 1380
Size: 41 1339
NspI r_CATG'y
Cuts at: 0 767 1121 1206 1380
Size: 767 354 85 174
Fragments arranged by size:
767 354 174 85
PfoI T'CCnGG_A
Cuts at: 0 188 1380
Size: 188 1192
PspOMI G'GGCC_C
Cuts at: 0 880 1380
Size: 880 500
PvuII CAG'CTG
Cuts at: 0 204 1380
Size: 204 1176
SalI G'TCGA_C
Cuts at: 0 574 1380
Size: 574 806
ScaI AGT'ACT
Cuts at: 0 1005 1380
Size: 1005 375
SfcI C'TryA_G
Cuts at: 0 835 1380
Size: 835 545
SmaI CCC'GGG
Cuts at: 0 8 1380
Size: 8 1372
SmlI C'TyrA_G
Cuts at: 0 713 1380
Size: 713 667
SspI AAT'ATT
Cuts at: 0 1179 1380
Size: 1179 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 347 1380
Size: 347 1033
TatI w'GTAC_w
Cuts at: 0 1003 1380
Size: 1003 377
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1040 1072 1380
Size: 1040 32 308
Fragments arranged by size:
1040 308 32
Tth111I GACn'n_nGTC
Cuts at: 0 368 1380
Size: 368 1012
XmaI C'CCGG_G
Cuts at: 0 6 1380
Size: 6 1374
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI AlwNI ApaI ApoI
AseI AvaI BanII BcgI BglII BlpI Bme1580I BmtI
BpmI BpuEI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrFI BssSI BstAPI BstYI
BtgZI ClaI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I Hin4I HincII HpaI MfeI MmeI MscI MslI
MspA1I NaeI NdeI NgoMIV NheI NotI NspI PfoI
PspOMI PvuII SalI ScaI SfcI SmaI SmlI SspI
TaqII TatI TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AloI ApaLI AscI AsiSI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BmgBI
BmrI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BseYI
BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtsI CspCI DraI
DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII KasI KpnI MluI NarI NcoI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI
StyI SwaI XbaI XcmI XhoI XmnI ZraI