Restriction map for
pFN21AE6424
(Linear) (Six-base) MAPSORT of: KIEE6424.seq Check: 8695 from: 1 to: 1659
KIEE6424.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1393 1659
Size: 1393 266
AccI GT'mk_AC
Cuts at: 0 1409 1659
Size: 1409 250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 175 779 1110 1657 1659
Size: 82 93 604 331 547 2
Fragments arranged by size:
604 547 331 93 82 2
AfeI AGC'GCT
Cuts at: 0 591 1159 1659
Size: 591 568 500
Fragments arranged by size:
591 568 500
AflIII A'CryG_T
Cuts at: 0 113 1478 1659
Size: 113 1365 181
Fragments arranged by size:
1365 181 113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 109 301 481 1659
Size: 109 192 180 1178
Fragments arranged by size:
1178 192 180 109
AlwNI CAG_nnn'CTG
Cuts at: 0 105 474 1519 1549 1659
Size: 105 369 1045 30 110
Fragments arranged by size:
1045 369 110 105 30
ApaI G_GGCC'C
Cuts at: 0 668 763 773 1659
Size: 668 95 10 886
Fragments arranged by size:
886 668 95 10
ApaLI G'TGCA_C
Cuts at: 0 1378 1659
Size: 1378 281
AvaI C'yCGr_G
Cuts at: 0 303 735 1279 1659
Size: 303 432 544 380
Fragments arranged by size:
544 432 380 303
BamHI G'GATC_C
Cuts at: 0 867 903 1659
Size: 867 36 756
Fragments arranged by size:
867 756 36
BanI G'GyrC_C
Cuts at: 0 59 234 705 1623 1659
Size: 59 175 471 918 36
Fragments arranged by size:
918 471 175 59 36
BanII G_rGCy'C
Cuts at: 0 559 620 668 763 773 950 1580 1659
Size: 559 61 48 95 10 177 630 79
Fragments arranged by size:
630 559 177 95 79 61 48 10
BbeI G_GCGC'C
Cuts at: 0 63 1659
Size: 63 1596
BbsI GAAGACnn'nnnn_
Cuts at: 0 1065 1659
Size: 1065 594
BbvCI CC'TCA_GC
Cuts at: 0 1550 1659
Size: 1550 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 72 106 1659
Size: 72 34 1553
Fragments arranged by size:
1553 72 34
BglI GCCn_nnn'nGGC
Cuts at: 0 777 830 1632 1659
Size: 777 53 802 27
Fragments arranged by size:
802 777 53 27
BlpI GC'TnA_GC
Cuts at: 0 619 1659
Size: 619 1040
Bme1580I G_kGCm'C
Cuts at: 0 239 460 668 708 763 773 1382 1628
Size: 239 221 208 40 55 10 609 246
Cuts at: 1628 1659
Size: 31
Fragments arranged by size:
609 246 239 221 208 55 40 31
10
BmgBI CAC'GTC
Cuts at: 0 1053 1461 1659
Size: 1053 408 198
Fragments arranged by size:
1053 408 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 400 1659
Size: 400 1259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 172 247 804 848 1006 1174 1435
Size: 127 45 75 557 44 158 168 261
Cuts at: 1435 1450 1659
Size: 15 209
Fragments arranged by size:
557 261 209 168 158 127 75 45
44 15
Bpu10I CC'TnA_GC
Cuts at: 0 1550 1574 1659
Size: 1550 24 85
Fragments arranged by size:
1550 85 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1060 1659
Size: 654 406 599
Fragments arranged by size:
654 599 406
BsaAI yAC'GTr
Cuts at: 0 116 1659
Size: 116 1543
BsaHI Gr'CG_yC
Cuts at: 0 60 79 1659
Size: 60 19 1580
Fragments arranged by size:
1580 60 19
BsaWI w'CCGG_w
Cuts at: 0 642 1659
Size: 642 1017
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 100 130 1659
Size: 100 30 1529
Fragments arranged by size:
1529 100 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 180 1003 1275 1408 1567 1623 1659
Size: 180 823 272 133 159 56 36
Fragments arranged by size:
823 272 180 159 133 56 36
BseYI C'CCAG_C
Cuts at: 0 474 872 908 1070 1225 1595 1659
Size: 474 398 36 162 155 370 64
Fragments arranged by size:
474 398 370 162 155 64 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1511 1659
Size: 1511 148
BsiEI CG_ry'CG
Cuts at: 0 57 1197 1370 1457 1659
Size: 57 1140 173 87 202
Fragments arranged by size:
1140 202 173 87 57
BsiHKAI G_wGCw'C
Cuts at: 0 88 176 620 680 700 1382 1659
Size: 88 88 444 60 20 682 277
Fragments arranged by size:
682 444 277 88 88 60 20
Bsp1286I G_dGCh'C
Cuts at: 0 88 176 239 460 559 620 668 680
Size: 88 88 63 221 99 61 48 12
Cuts at: 680 700 708 763 773 950 1382 1580 1628
Size: 20 8 55 10 177 432 198 48
Cuts at: 1628 1659
Size: 31
Fragments arranged by size:
432 221 198 177 99 88 88 63
61 55 48 48 31 20 12 10
8
BspEI T'CCGG_A
Cuts at: 0 642 1659
Size: 642 1017
BspHI T'CATG_A
Cuts at: 0 512 1659
Size: 512 1147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 1393 1659
Size: 215 1178 266
Fragments arranged by size:
1178 266 215
BsrBI CCG'CTC
Cuts at: 0 894 900 924 930 993 1086 1372 1659
Size: 894 6 24 6 63 93 286 287
Fragments arranged by size:
894 287 286 93 63 24 6 6
BsrDI GCAATG_nn'
Cuts at: 0 1507 1659
Size: 1507 152
BsrFI r'CCGG_y
Cuts at: 0 56 119 963 1398 1644 1659
Size: 56 63 844 435 246 15
Fragments arranged by size:
844 435 246 63 56 15
BsrGI T'GTAC_A
Cuts at: 0 416 1659
Size: 416 1243
BssSI C'ACGA_G
Cuts at: 0 420 774 1659
Size: 420 354 885
Fragments arranged by size:
885 420 354
BstEII G'GTnAC_C
Cuts at: 0 1113 1659
Size: 1113 546
BstYI r'GATC_y
Cuts at: 0 867 903 1659
Size: 867 36 756
Fragments arranged by size:
867 756 36
Bsu36I CC'TnA_GG
Cuts at: 0 660 1659
Size: 660 999
BtgI C'CryG_G
Cuts at: 0 29 530 624 935 950 1659
Size: 29 501 94 311 15 709
Fragments arranged by size:
709 501 311 94 29 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 264 1659
Size: 264 1395
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 584 619 1659
Size: 584 35 1040
Fragments arranged by size:
1040 584 35
DraIII CAC_nnn'GTG
Cuts at: 0 69 1659
Size: 69 1590
DrdI GACnn_nn'nnGTC
Cuts at: 0 468 1201 1659
Size: 468 733 458
Fragments arranged by size:
733 468 458
EaeI y'GGCC_r
Cuts at: 0 54 573 1006 1367 1454 1469 1646 1659
Size: 54 519 433 361 87 15 177 13
Fragments arranged by size:
519 433 361 177 87 54 15 13
EagI C'GGCC_G
Cuts at: 0 54 1367 1454 1659
Size: 54 1313 87 205
Fragments arranged by size:
1313 205 87 54
EarI CTCTTCn'nnn_
Cuts at: 0 1366 1600 1659
Size: 1366 234 59
Fragments arranged by size:
1366 234 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 35 1210 1659
Size: 35 1175 449
Fragments arranged by size:
1175 449 35
EcoICRI GAG'CTC
Cuts at: 0 618 1659
Size: 618 1041
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 127 172 175 247 779 804 848
Size: 82 45 45 3 72 532 25 44
Cuts at: 848 1006 1110 1174 1435 1450 1657 1659
Size: 158 104 64 261 15 207 2
Fragments arranged by size:
532 261 207 158 104 82 72 64
45 45 44 25 15 3 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1348 1618 1659
Size: 1348 270 41
Fragments arranged by size:
1348 270 41
EcoO109I rG'GnC_Cy
Cuts at: 0 259 664 665 759 760 832 1182 1496
Size: 259 405 1 94 1 72 350 314
Cuts at: 1496 1659
Size: 163
Fragments arranged by size:
405 350 314 259 163 94 72 1
1
HaeII r_GCGC'y
Cuts at: 0 63 497 593 1095 1161 1564 1659
Size: 63 434 96 502 66 403 95
Fragments arranged by size:
502 434 403 96 95 66 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 1659
Size: 286 32 1341
Fragments arranged by size:
1341 286 32
KasI G'GCGC_C
Cuts at: 0 59 1659
Size: 59 1600
MscI TGG'CCA
Cuts at: 0 575 1008 1471 1659
Size: 575 433 463 188
Fragments arranged by size:
575 463 433 188
MspA1I CmG'CkG
Cuts at: 0 31 291 626 715 1012 1546 1659
Size: 31 260 335 89 297 534 113
Fragments arranged by size:
534 335 297 260 113 89 31
NaeI GCC'GGC
Cuts at: 0 58 121 1400 1646 1659
Size: 58 63 1279 246 13
Fragments arranged by size:
1279 246 63 58 13
NarI GG'CG_CC
Cuts at: 0 60 1659
Size: 60 1599
NgoMIV G'CCGG_C
Cuts at: 0 56 119 1398 1644 1659
Size: 56 63 1279 246 15
Fragments arranged by size:
1279 246 63 56 15
NspI r_CATG'y
Cuts at: 0 1438 1482 1659
Size: 1438 44 177
Fragments arranged by size:
1438 177 44
PasI CC'CwG_GG
Cuts at: 0 1585 1659
Size: 1585 74
PciI A'CATG_T
Cuts at: 0 1478 1659
Size: 1478 181
PfoI T'CCnGG_A
Cuts at: 0 244 1322 1659
Size: 244 1078 337
Fragments arranged by size:
1078 337 244
PmlI CAC'GTG
Cuts at: 0 116 1659
Size: 116 1543
PpuMI rG'GwC_Cy
Cuts at: 0 1182 1496 1659
Size: 1182 314 163
Fragments arranged by size:
1182 314 163
PspOMI G'GGCC_C
Cuts at: 0 664 759 769 1659
Size: 664 95 10 890
Fragments arranged by size:
890 664 95 10
PspXI vC'TCGA_Gb
Cuts at: 0 303 1659
Size: 303 1356
PstI C_TGCA'G
Cuts at: 0 386 470 1001 1659
Size: 386 84 531 658
Fragments arranged by size:
658 531 386 84
PvuII CAG'CTG
Cuts at: 0 291 715 1546 1659
Size: 291 424 831 113
Fragments arranged by size:
831 424 291 113
SacI G_AGCT'C
Cuts at: 0 620 1659
Size: 620 1039
SacII CC_GC'GG
Cuts at: 0 32 627 1659
Size: 32 595 1032
Fragments arranged by size:
1032 595 32
SanDI GG'GwC_CC
Cuts at: 0 1496 1659
Size: 1496 163
SfcI C'TryA_G
Cuts at: 0 382 466 997 1207 1659
Size: 382 84 531 210 452
Fragments arranged by size:
531 452 382 210 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 777 1659
Size: 777 882
SfoI GGC'GCC
Cuts at: 0 61 1659
Size: 61 1598
SmlI C'TyrA_G
Cuts at: 0 303 633 1075 1659
Size: 303 330 442 584
Fragments arranged by size:
584 442 330 303
SphI G_CATG'C
Cuts at: 0 1438 1659
Size: 1438 221
StuI AGG'CCT
Cuts at: 0 780 1003 1123 1659
Size: 780 223 120 536
Fragments arranged by size:
780 536 223 120
StyI C'CwwG_G
Cuts at: 0 602 1109 1118 1659
Size: 602 507 9 541
Fragments arranged by size:
602 541 507 9
TatI w'GTAC_w
Cuts at: 0 416 1659
Size: 416 1243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 100 132 1659
Size: 100 32 1527
Fragments arranged by size:
1527 100 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 228 1233 1659
Size: 228 1005 426
Fragments arranged by size:
1005 426 228
XhoI C'TCGA_G
Cuts at: 0 303 1659
Size: 303 1356
XmnI GAAnn'nnTTC
Cuts at: 0 1360 1659
Size: 1360 299
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstEII BstYI Bsu36I BtgI BtgZI
CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmlI
PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI
SfcI SfiI SfoI SmlI SphI StuI StyI TatI
TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BciVI BclI
BfrBI BglII BmtI BplI BsaI BsaBI BsiWI BsmI
BsmBI BssHII BstAPI BstBI BstXI BstZ17I BtsI ClaI
DraI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PmeI
PpiI PshAI PsiI PsrI PvuI RsrII SalI SapI
SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TaqII Tth111I XbaI XmaI ZraI