Restriction map for
pFN21AE6423
(Linear) (Six-base) MAPSORT of: KIEE6423.seq Check: 4430 from: 1 to: 1395
KIEE6423.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 222 1395
Size: 222 1173
AccI GT'mk_AC
Cuts at: 0 317 1217 1395
Size: 317 900 178
Fragments arranged by size:
900 317 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 475 513 871 1395
Size: 289 186 38 358 524
Fragments arranged by size:
524 358 289 186 38
AfeI AGC'GCT
Cuts at: 0 301 460 1395
Size: 301 159 935
Fragments arranged by size:
935 301 159
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1159 1191 1395
Size: 1159 32 204
Fragments arranged by size:
1159 204 32
AlwNI CAG_nnn'CTG
Cuts at: 0 210 861 1395
Size: 210 651 534
Fragments arranged by size:
651 534 210
ApaI G_GGCC'C
Cuts at: 0 1035 1395
Size: 1035 360
ApoI r'AATT_y
Cuts at: 0 586 880 1395
Size: 586 294 515
Fragments arranged by size:
586 515 294
BamHI G'GATC_C
Cuts at: 0 78 1395
Size: 78 1317
BanI G'GyrC_C
Cuts at: 0 384 1395
Size: 384 1011
BanII G_rGCy'C
Cuts at: 0 122 242 453 1035 1395
Size: 122 120 211 582 360
Fragments arranged by size:
582 360 211 122 120
BbsI GAAGACnn'nnnn_
Cuts at: 0 336 1395
Size: 336 1059
BbvCI CC'TCA_GC
Cuts at: 0 53 1395
Size: 53 1342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 789 823 886 920 1143 1177 1395
Size: 789 34 63 34 223 34 218
Fragments arranged by size:
789 223 218 63 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 901 1395
Size: 901 494
BglII A'GATC_T
Cuts at: 0 93 753 1395
Size: 93 660 642
Fragments arranged by size:
660 642 93
BlpI GC'TnA_GC
Cuts at: 0 1318 1395
Size: 1318 77
Bme1580I G_kGCm'C
Cuts at: 0 1035 1277 1395
Size: 1035 242 118
Fragments arranged by size:
1035 242 118
BmgBI CAC'GTC
Cuts at: 0 936 1215 1395
Size: 936 279 180
Fragments arranged by size:
936 279 180
BmrI ACTGGGnnnn_n'
Cuts at: 0 146 1395
Size: 146 1249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1135 1395
Size: 1135 260
Bpu10I CC'TnA_GC
Cuts at: 0 53 1395
Size: 53 1342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1395
Size: 496 899
BsaAI yAC'GTr
Cuts at: 0 839 1395
Size: 839 556
BsaHI Gr'CG_yC
Cuts at: 0 88 1395
Size: 88 1307
BsaWI w'CCGG_w
Cuts at: 0 246 674 1395
Size: 246 428 721
Fragments arranged by size:
721 428 246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1159 1189 1395
Size: 1159 30 206
Fragments arranged by size:
1159 206 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 459 1395
Size: 85 374 936
Fragments arranged by size:
936 374 85
BseYI C'CCAG_C
Cuts at: 0 144 411 630 1395
Size: 144 267 219 765
Fragments arranged by size:
765 267 219 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 250 346 565 890 1021 1174 1395
Size: 154 96 96 219 325 131 153 221
Fragments arranged by size:
325 221 219 154 153 131 96 96
BsiHKAI G_wGCw'C
Cuts at: 0 242 1366 1395
Size: 242 1124 29
Fragments arranged by size:
1124 242 29
BsmI GAATG_Cn'
Cuts at: 0 164 413 1395
Size: 164 249 982
Fragments arranged by size:
982 249 164
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1192 1395
Size: 1192 203
Bsp1286I G_dGCh'C
Cuts at: 0 122 242 453 1035 1277 1366 1395
Size: 122 120 211 582 242 89 29
Fragments arranged by size:
582 242 211 122 120 89 29
BspEI T'CCGG_A
Cuts at: 0 246 674 1395
Size: 246 428 721
Fragments arranged by size:
721 428 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 129 222 1229 1395
Size: 129 93 1007 166
Fragments arranged by size:
1007 166 129 93
BsrBI CCG'CTC
Cuts at: 0 720 823 996 1395
Size: 720 103 173 399
Fragments arranged by size:
720 399 173 103
BstAPI GCAn_nnn'nTGC
Cuts at: 0 210 1395
Size: 210 1185
BstYI r'GATC_y
Cuts at: 0 78 93 753 963 1265 1395
Size: 78 15 660 210 302 130
Fragments arranged by size:
660 302 210 130 78 15
BstZ17I GTA'TAC
Cuts at: 0 318 1395
Size: 318 1077
Bsu36I CC'TnA_GG
Cuts at: 0 1306 1395
Size: 1306 89
BtgI C'CryG_G
Cuts at: 0 29 1103 1395
Size: 29 1074 292
Fragments arranged by size:
1074 292 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 28 1395
Size: 28 1367
DrdI GACnn_nn'nnGTC
Cuts at: 0 340 1395
Size: 340 1055
EaeI y'GGCC_r
Cuts at: 0 6 215 1395
Size: 6 209 1180
Fragments arranged by size:
1180 209 6
EarI CTCTTCn'nnn_
Cuts at: 0 268 523 920 961 1051 1395
Size: 268 255 397 41 90 344
Fragments arranged by size:
397 344 268 255 90 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 73 91 1395
Size: 73 18 1304
Fragments arranged by size:
1304 73 18
EcoICRI GAG'CTC
Cuts at: 0 240 1395
Size: 240 1155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 475 513 871 1135 1395
Size: 289 186 38 358 264 260
Fragments arranged by size:
358 289 264 260 186 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 853 1192 1395
Size: 853 339 203
Fragments arranged by size:
853 339 203
EcoO109I rG'GnC_Cy
Cuts at: 0 141 258 845 984 1032 1395
Size: 141 117 587 139 48 363
Fragments arranged by size:
587 363 141 139 117 48
EcoRI G'AATT_C
Cuts at: 0 586 880 1395
Size: 586 294 515
Fragments arranged by size:
586 515 294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 279 1395
Size: 279 1116
HaeII r_GCGC'y
Cuts at: 0 303 462 1076 1395
Size: 303 159 614 319
Fragments arranged by size:
614 319 303 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1084 1116 1395
Size: 1084 32 279
Fragments arranged by size:
1084 279 32
HincII GTy'rAC
Cuts at: 0 468 1257 1395
Size: 468 789 138
Fragments arranged by size:
789 468 138
HindIII A'AGCT_T
Cuts at: 0 700 1395
Size: 700 695
MscI TGG'CCA
Cuts at: 0 217 1395
Size: 217 1178
MspA1I CmG'CkG
Cuts at: 0 24 31 52 183 533 634 807 1029
Size: 24 7 21 131 350 101 173 222
Cuts at: 1029 1113 1322 1395
Size: 84 209 73
Fragments arranged by size:
350 222 209 173 131 101 84 73
24 21 7
NruI TCG'CGA
Cuts at: 0 707 1395
Size: 707 688
NspI r_CATG'y
Cuts at: 0 277 1395
Size: 277 1118
PfoI T'CCnGG_A
Cuts at: 0 347 1395
Size: 347 1048
PmlI CAC'GTG
Cuts at: 0 839 1395
Size: 839 556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1159 1191 1395
Size: 1159 32 204
Fragments arranged by size:
1159 204 32
PpuMI rG'GwC_Cy
Cuts at: 0 141 845 984 1395
Size: 141 704 139 411
Fragments arranged by size:
704 411 141 139
PspOMI G'GGCC_C
Cuts at: 0 1031 1395
Size: 1031 364
PstI C_TGCA'G
Cuts at: 0 39 206 974 1395
Size: 39 167 768 421
Fragments arranged by size:
768 421 167 39
PvuII CAG'CTG
Cuts at: 0 52 634 1113 1322 1395
Size: 52 582 479 209 73
Fragments arranged by size:
582 479 209 73 52
SacI G_AGCT'C
Cuts at: 0 242 1395
Size: 242 1153
SacII CC_GC'GG
Cuts at: 0 32 1395
Size: 32 1363
SanDI GG'GwC_CC
Cuts at: 0 845 1395
Size: 845 550
SapI GCTCTTCn'nnn_
Cuts at: 0 523 1395
Size: 523 872
SbfI CC_TGCA'GG
Cuts at: 0 206 1395
Size: 206 1189
SexAI A'CCwGG_T
Cuts at: 0 470 1395
Size: 470 925
SfcI C'TryA_G
Cuts at: 0 35 202 970 1395
Size: 35 167 768 425
Fragments arranged by size:
768 425 167 35
SmlI C'TyrA_G
Cuts at: 0 511 1395
Size: 511 884
StuI AGG'CCT
Cuts at: 0 869 1395
Size: 869 526
StyI C'CwwG_G
Cuts at: 0 617 1269 1395
Size: 617 652 126
Fragments arranged by size:
652 617 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 414 1395
Size: 414 981
TatI w'GTAC_w
Cuts at: 0 1043 1181 1395
Size: 1043 138 214
Fragments arranged by size:
1043 214 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1369 1395
Size: 1369 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 408 1395
Size: 408 987
XmnI GAAnn'nnTTC
Cuts at: 0 460 955 1171 1395
Size: 460 495 216 224
Fragments arranged by size:
495 460 224 216
Enzymes that do cut:
AarI AccI AcuI AfeI AloI AlwNI ApaI ApoI
BamHI BanI BanII BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BstAPI BstYI BstZ17I
Bsu36I BtgI BtgZI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII MscI MspA1I NruI NspI PfoI PmlI PpiI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SbfI SexAI SfcI SmlI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BciVI BclI BfrBI BmtI BplI BsaI BsaBI
BsiEI BsiWI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BtsI ClaI CspCI DraI DraIII
EagI EcoRV FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI PacI PasI PciI PflMI
PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
ZraI