Restriction map for
pFN21AE6421
(Linear) (Six-base) MAPSORT of: KIEE6421.seq Check: 4532 from: 1 to: 1674
KIEE6421.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 153 1674
Size: 153 1521
AccI GT'mk_AC
Cuts at: 0 44 1195 1674
Size: 44 1151 479
Fragments arranged by size:
1151 479 44
Acc65I G'GTAC_C
Cuts at: 0 1599 1674
Size: 1599 75
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1674
Size: 351 1323
AfeI AGC'GCT
Cuts at: 0 697 1292 1674
Size: 697 595 382
Fragments arranged by size:
697 595 382
AflIII A'CryG_T
Cuts at: 0 1244 1246 1674
Size: 1244 2 428
Fragments arranged by size:
1244 428 2
AgeI A'CCGG_T
Cuts at: 0 1596 1624 1674
Size: 1596 28 50
Fragments arranged by size:
1596 50 28
AhdI GACnn_n'nnGTC
Cuts at: 0 426 642 912 1674
Size: 426 216 270 762
Fragments arranged by size:
762 426 270 216
AleI CACnn'nnGTG
Cuts at: 0 850 1231 1674
Size: 850 381 443
Fragments arranged by size:
850 443 381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1120 1674
Size: 1120 554
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 1674
Size: 196 32 1446
Fragments arranged by size:
1446 196 32
AlwNI CAG_nnn'CTG
Cuts at: 0 609 1475 1674
Size: 609 866 199
Fragments arranged by size:
866 609 199
ApaI G_GGCC'C
Cuts at: 0 1538 1674
Size: 1538 136
ApaLI G'TGCA_C
Cuts at: 0 121 1674
Size: 121 1553
ApoI r'AATT_y
Cuts at: 0 544 1674
Size: 544 1130
AvaI C'yCGr_G
Cuts at: 0 52 175 1342 1674
Size: 52 123 1167 332
Fragments arranged by size:
1167 332 123 52
BanI G'GyrC_C
Cuts at: 0 165 316 390 852 1032 1395 1599 1674
Size: 165 151 74 462 180 363 204 75
Fragments arranged by size:
462 363 204 180 165 151 75 74
BanII G_rGCy'C
Cuts at: 0 34 761 864 872 1487 1538 1658 1674
Size: 34 727 103 8 615 51 120 16
Fragments arranged by size:
727 615 120 103 51 34 16 8
BbeI G_GCGC'C
Cuts at: 0 394 1036 1399 1674
Size: 394 642 363 275
Fragments arranged by size:
642 394 363 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1437 1471 1674
Size: 1437 34 203
Fragments arranged by size:
1437 203 34
BglI GCCn_nnn'nGGC
Cuts at: 0 64 1674
Size: 64 1610
BlpI GC'TnA_GC
Cuts at: 0 1378 1674
Size: 1378 296
Bme1580I G_kGCm'C
Cuts at: 0 125 170 319 857 1538 1674
Size: 125 45 149 538 681 136
Fragments arranged by size:
681 538 149 136 125 45
BmgBI CAC'GTC
Cuts at: 0 39 131 1674
Size: 39 92 1543
Fragments arranged by size:
1543 92 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 9 483 1096 1674
Size: 9 474 613 578
Fragments arranged by size:
613 578 474 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1215 1674
Size: 1215 459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 787 829 1674
Size: 631 156 42 845
Fragments arranged by size:
845 631 156 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 886 955 1528 1674
Size: 100 786 69 573 146
Fragments arranged by size:
786 573 146 100 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 839 1674
Size: 839 835
BsaAI yAC'GTr
Cuts at: 0 1164 1247 1674
Size: 1164 83 427
Fragments arranged by size:
1164 427 83
BsaHI Gr'CG_yC
Cuts at: 0 338 391 1033 1396 1674
Size: 338 53 642 363 278
Fragments arranged by size:
642 363 338 278 53
BsaWI w'CCGG_w
Cuts at: 0 80 149 641 1596 1624 1674
Size: 80 69 492 955 28 50
Fragments arranged by size:
955 492 80 69 50 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 478 508 1674
Size: 196 30 252 30 1166
Fragments arranged by size:
1166 252 196 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1666 1674
Size: 1666 8
BseYI C'CCAG_C
Cuts at: 0 194 416 1212 1537 1674
Size: 194 222 796 325 137
Fragments arranged by size:
796 325 222 194 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1222 1311 1605 1674
Size: 1111 111 89 294 69
Fragments arranged by size:
1111 294 111 89 69
BsiEI CG_ry'CG
Cuts at: 0 276 1674
Size: 276 1398
BsiHKAI G_wGCw'C
Cuts at: 0 125 761 864 872 1109 1674
Size: 125 636 103 8 237 565
Fragments arranged by size:
636 565 237 125 103 8
BsiWI C'GTAC_G
Cuts at: 0 1160 1674
Size: 1160 514
Bsp1286I G_dGCh'C
Cuts at: 0 34 125 170 319 761 857 864 872
Size: 34 91 45 149 442 96 7 8
Cuts at: 872 1109 1487 1538 1658 1674
Size: 237 378 51 120 16
Fragments arranged by size:
442 378 237 149 120 96 91 51
45 34 16 8 7
BspEI T'CCGG_A
Cuts at: 0 149 1674
Size: 149 1525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 153 749 1674
Size: 153 596 925
Fragments arranged by size:
925 596 153
BsrBI CCG'CTC
Cuts at: 0 1049 1674
Size: 1049 625
BsrDI GCAATG_nn'
Cuts at: 0 1036 1674
Size: 1036 638
BsrFI r'CCGG_y
Cuts at: 0 1596 1624 1674
Size: 1596 28 50
Fragments arranged by size:
1596 50 28
BssHII G'CGCG_C
Cuts at: 0 456 566 568 570 1179 1181 1674
Size: 456 110 2 2 609 2 493
Fragments arranged by size:
609 493 456 110 2 2 2
BssSI C'ACGA_G
Cuts at: 0 403 1674
Size: 403 1271
BstXI CCAn_nnnn'nTGG
Cuts at: 0 807 1230 1281 1674
Size: 807 423 51 393
Fragments arranged by size:
807 423 393 51
BstYI r'GATC_y
Cuts at: 0 617 1674
Size: 617 1057
BstZ17I GTA'TAC
Cuts at: 0 1196 1674
Size: 1196 478
Bsu36I CC'TnA_GG
Cuts at: 0 1468 1674
Size: 1468 206
BtgI C'CryG_G
Cuts at: 0 227 383 794 1559 1674
Size: 227 156 411 765 115
Fragments arranged by size:
765 411 227 156 115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 463 534 1674
Size: 463 71 1140
Fragments arranged by size:
1140 463 71
BtsI GCAGTG_nn'
Cuts at: 0 1134 1674
Size: 1134 540
DraIII CAC_nnn'GTG
Cuts at: 0 1140 1365 1674
Size: 1140 225 309
Fragments arranged by size:
1140 309 225
EaeI y'GGCC_r
Cuts at: 0 236 273 440 496 554 797 1265 1674
Size: 236 37 167 56 58 243 468 409
Fragments arranged by size:
468 409 243 236 167 58 56 37
EagI C'GGCC_G
Cuts at: 0 273 1674
Size: 273 1401
EarI CTCTTCn'nnn_
Cuts at: 0 793 1118 1393 1674
Size: 793 325 275 281
Fragments arranged by size:
793 325 281 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 914 1232 1429 1674
Size: 914 318 197 245
Fragments arranged by size:
914 318 245 197
EcoICRI GAG'CTC
Cuts at: 0 759 862 870 1674
Size: 759 103 8 804
Fragments arranged by size:
804 759 103 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 631 787 829 1674
Size: 351 280 156 42 845
Fragments arranged by size:
845 351 280 156 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 442 1390 1674
Size: 442 948 284
Fragments arranged by size:
948 442 284
EcoO109I rG'GnC_Cy
Cuts at: 0 510 1472 1534 1674
Size: 510 962 62 140
Fragments arranged by size:
962 510 140 62
EcoRI G'AATT_C
Cuts at: 0 544 1674
Size: 544 1130
FspI TGC'GCA
Cuts at: 0 602 748 1674
Size: 602 146 926
Fragments arranged by size:
926 602 146
HaeII r_GCGC'y
Cuts at: 0 394 699 1036 1294 1399 1530 1674
Size: 394 305 337 258 105 131 144
Fragments arranged by size:
394 337 305 258 144 131 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 628 660 898 930 1012 1044
Size: 412 32 184 32 238 32 82 32
Cuts at: 1044 1674
Size: 630
Fragments arranged by size:
630 412 238 184 82 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 390 1032 1395 1674
Size: 390 642 363 279
Fragments arranged by size:
642 390 363 279
KpnI G_GTAC'C
Cuts at: 0 1603 1674
Size: 1603 71
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1533 1674
Size: 1533 141
MscI TGG'CCA
Cuts at: 0 238 799 1674
Size: 238 561 875
Fragments arranged by size:
875 561 238
MslI CAynn'nnrTG
Cuts at: 0 850 1231 1333 1674
Size: 850 381 102 341
Fragments arranged by size:
850 381 341 102
MspA1I CmG'CkG
Cuts at: 0 229 272 609 1451 1606 1674
Size: 229 43 337 842 155 68
Fragments arranged by size:
842 337 229 155 68 43
NarI GG'CG_CC
Cuts at: 0 391 1033 1396 1674
Size: 391 642 363 278
Fragments arranged by size:
642 391 363 278
NcoI C'CATG_G
Cuts at: 0 383 1674
Size: 383 1291
NotI GC'GGCC_GC
Cuts at: 0 273 1674
Size: 273 1401
NspI r_CATG'y
Cuts at: 0 1506 1674
Size: 1506 168
PasI CC'CwG_GG
Cuts at: 0 1531 1674
Size: 1531 143
PflMI CCAn_nnn'nTGG
Cuts at: 0 1140 1674
Size: 1140 534
PfoI T'CCnGG_A
Cuts at: 0 806 1508 1674
Size: 806 702 166
Fragments arranged by size:
806 702 166
PmlI CAC'GTG
Cuts at: 0 1247 1674
Size: 1247 427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 1674
Size: 196 32 1446
Fragments arranged by size:
1446 196 32
PshAI GACnn'nnGTC
Cuts at: 0 91 928 1674
Size: 91 837 746
Fragments arranged by size:
837 746 91
PspOMI G'GGCC_C
Cuts at: 0 1534 1674
Size: 1534 140
PstI C_TGCA'G
Cuts at: 0 485 1674
Size: 485 1189
PvuII CAG'CTG
Cuts at: 0 609 1451 1674
Size: 609 842 223
Fragments arranged by size:
842 609 223
RsrII CG'GwC_CG
Cuts at: 0 152 1674
Size: 152 1522
SacI G_AGCT'C
Cuts at: 0 761 864 872 1674
Size: 761 103 8 802
Fragments arranged by size:
802 761 103 8
SacII CC_GC'GG
Cuts at: 0 230 1674
Size: 230 1444
SapI GCTCTTCn'nnn_
Cuts at: 0 793 1118 1674
Size: 793 325 556
Fragments arranged by size:
793 556 325
SfcI C'TryA_G
Cuts at: 0 481 1674
Size: 481 1193
SfoI GGC'GCC
Cuts at: 0 392 1034 1397 1674
Size: 392 642 363 277
Fragments arranged by size:
642 392 363 277
SgrAI Cr'CCGG_yG
Cuts at: 0 1624 1674
Size: 1624 50
SmlI C'TyrA_G
Cuts at: 0 115 865 970 1543 1674
Size: 115 750 105 573 131
Fragments arranged by size:
750 573 131 115 105
SpeI A'CTAG_T
Cuts at: 0 1401 1674
Size: 1401 273
StuI AGG'CCT
Cuts at: 0 719 1674
Size: 719 955
StyI C'CwwG_G
Cuts at: 0 383 1674
Size: 383 1291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 751 1156 1287 1315 1674
Size: 751 405 131 28 359
Fragments arranged by size:
751 405 359 131 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 115 147 478 510 1674
Size: 115 32 331 32 1164
Fragments arranged by size:
1164 331 115 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 40 664 978 1674
Size: 40 624 314 696
Fragments arranged by size:
696 624 314 40
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1071 1674
Size: 1071 603
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NarI NcoI NotI NspI PasI PflMI PfoI
PmlI PpiI PshAI PspOMI PstI PvuII RsrII SacI
SacII SapI SfcI SfoI SgrAI SmlI SpeI StuI
StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII AclI AflII AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmtI
Bpu10I BsaBI BsmI BsmBI BspHI BsrGI BstAPI BstBI
BstEII ClaI CspCI DraI DrdI EcoRV FalI FseI
FspAI HincII HindIII HpaI MfeI MluI NaeI NdeI
NgoMIV NheI NruI NsiI PacI PciI PmeI PpuMI
PsiI PspXI PsrI PvuI SalI SanDI SbfI ScaI
SexAI SfiI SgfI SmaI SnaBI SphI SrfI SspI
SwaI TatI XbaI XhoI XmaI XmnI ZraI