Restriction map for
pFN21AE6418
(Linear) (Six-base) MAPSORT of: KIEE6418.seq Check: 3436 from: 1 to: 1869
KIEE6418.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 663 1869
Size: 663 1206
AccI GT'mk_AC
Cuts at: 0 494 1213 1869
Size: 494 719 656
Fragments arranged by size:
719 656 494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 669 928 1339 1701 1869
Size: 669 259 411 362 168
Fragments arranged by size:
669 411 362 259 168
AleI CACnn'nnGTG
Cuts at: 0 1273 1869
Size: 1273 596
ApaI G_GGCC'C
Cuts at: 0 1292 1869
Size: 1292 577
ApoI r'AATT_y
Cuts at: 0 1249 1745 1869
Size: 1249 496 124
Fragments arranged by size:
1249 496 124
AvaI C'yCGr_G
Cuts at: 0 209 443 1869
Size: 209 234 1426
Fragments arranged by size:
1426 234 209
BanII G_rGCy'C
Cuts at: 0 575 726 1292 1537 1841 1869
Size: 575 151 566 245 304 28
Fragments arranged by size:
575 566 304 245 151 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 949 1087 1869
Size: 949 138 782
Fragments arranged by size:
949 782 138
BclI T'GATC_A
Cuts at: 0 930 1869
Size: 930 939
BglI GCCn_nnn'nGGC
Cuts at: 0 607 1054 1063 1588 1869
Size: 607 447 9 525 281
Fragments arranged by size:
607 525 447 281 9
BglII A'GATC_T
Cuts at: 0 1425 1869
Size: 1425 444
Bme1580I G_kGCm'C
Cuts at: 0 410 1292 1869
Size: 410 882 577
Fragments arranged by size:
882 577 410
BmrI ACTGGGnnnn_n'
Cuts at: 0 652 996 1526 1869
Size: 652 344 530 343
Fragments arranged by size:
652 530 344 343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 607 717 979 1352 1805 1869
Size: 535 72 110 262 373 453 64
Fragments arranged by size:
535 453 373 262 110 72 64
Bpu10I CC'TnA_GC
Cuts at: 0 416 1869
Size: 416 1453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1798 1869
Size: 1798 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 1516 1642 1869
Size: 139 1377 126 227
Fragments arranged by size:
1377 227 139 126
BsaHI Gr'CG_yC
Cuts at: 0 224 1658 1869
Size: 224 1434 211
Fragments arranged by size:
1434 224 211
BsaWI w'CCGG_w
Cuts at: 0 1541 1869
Size: 1541 328
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 83 113 1869
Size: 83 30 1756
Fragments arranged by size:
1756 83 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 604 976 1849 1869
Size: 604 372 873 20
Fragments arranged by size:
873 604 372 20
BseYI C'CCAG_C
Cuts at: 0 1841 1869
Size: 1841 28
BsiHKAI G_wGCw'C
Cuts at: 0 422 575 1811 1869
Size: 422 153 1236 58
Fragments arranged by size:
1236 422 153 58
BsmBI CGTCTCn'nnnn_
Cuts at: 0 846 1869
Size: 846 1023
Bsp1286I G_dGCh'C
Cuts at: 0 410 422 575 726 1292 1537 1811 1841
Size: 410 12 153 151 566 245 274 30
Cuts at: 1841 1869
Size: 28
Fragments arranged by size:
566 410 274 245 153 151 30 28
12
BspEI T'CCGG_A
Cuts at: 0 1541 1869
Size: 1541 328
BspHI T'CATG_A
Cuts at: 0 540 1869
Size: 540 1329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 663 1722 1869
Size: 663 1059 147
Fragments arranged by size:
1059 663 147
BsrBI CCG'CTC
Cuts at: 0 504 531 1869
Size: 504 27 1338
Fragments arranged by size:
1338 504 27
BsrDI GCAATG_nn'
Cuts at: 0 1022 1869
Size: 1022 847
BssSI C'ACGA_G
Cuts at: 0 1271 1869
Size: 1271 598
BstXI CCAn_nnnn'nTGG
Cuts at: 0 774 1638 1827 1869
Size: 774 864 189 42
Fragments arranged by size:
864 774 189 42
BstYI r'GATC_y
Cuts at: 0 629 1169 1425 1869
Size: 629 540 256 444
Fragments arranged by size:
629 540 444 256
BstZ17I GTA'TAC
Cuts at: 0 1214 1869
Size: 1214 655
BtsI GCAGTG_nn'
Cuts at: 0 1050 1869
Size: 1050 819
DraI TTT'AAA
Cuts at: 0 356 1869
Size: 356 1513
DraIII CAC_nnn'GTG
Cuts at: 0 1162 1778 1866 1869
Size: 1162 616 88 3
Fragments arranged by size:
1162 616 88 3
EaeI y'GGCC_r
Cuts at: 0 14 1869
Size: 14 1855
EarI CTCTTCn'nnn_
Cuts at: 0 970 1319 1618 1869
Size: 970 349 299 251
Fragments arranged by size:
970 349 299 251
EcoICRI GAG'CTC
Cuts at: 0 573 1869
Size: 573 1296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 607 669 717 928 979 1339 1352
Size: 535 72 62 48 211 51 360 13
Cuts at: 1352 1701 1805 1869
Size: 349 104 64
Fragments arranged by size:
535 360 349 211 104 72 64 62
51 48 13
EcoO109I rG'GnC_Cy
Cuts at: 0 620 1288 1856 1869
Size: 620 668 568 13
Fragments arranged by size:
668 620 568 13
EcoRV GAT'ATC
Cuts at: 0 1644 1869
Size: 1644 225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 489 846 1869
Size: 489 357 1023
Fragments arranged by size:
1023 489 357
HaeII r_GCGC'y
Cuts at: 0 585 1869
Size: 585 1284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 83 115 1020 1052 1496 1528 1869
Size: 83 32 905 32 444 32 341
Fragments arranged by size:
905 444 341 83 32 32 32
HincII GTy'rAC
Cuts at: 0 495 1310 1869
Size: 495 815 559
Fragments arranged by size:
815 559 495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 779 1869
Size: 32 747 1090
Fragments arranged by size:
1090 747 32
MscI TGG'CCA
Cuts at: 0 16 1869
Size: 16 1853
MslI CAynn'nnrTG
Cuts at: 0 545 889 1043 1189 1273 1373 1869
Size: 545 344 154 146 84 100 496
Fragments arranged by size:
545 496 344 154 146 100 84
MspA1I CmG'CkG
Cuts at: 0 63 1416 1869
Size: 63 1353 453
Fragments arranged by size:
1353 453 63
NspI r_CATG'y
Cuts at: 0 894 1611 1869
Size: 894 717 258
Fragments arranged by size:
894 717 258
PflMI CCAn_nnn'nTGG
Cuts at: 0 1162 1869
Size: 1162 707
PfoI T'CCnGG_A
Cuts at: 0 752 1616 1869
Size: 752 864 253
Fragments arranged by size:
864 752 253
PpuMI rG'GwC_Cy
Cuts at: 0 1856 1869
Size: 1856 13
PspOMI G'GGCC_C
Cuts at: 0 1288 1869
Size: 1288 581
PspXI vC'TCGA_Gb
Cuts at: 0 209 1869
Size: 209 1660
PvuII CAG'CTG
Cuts at: 0 1416 1869
Size: 1416 453
SacI G_AGCT'C
Cuts at: 0 575 1869
Size: 575 1294
SalI G'TCGA_C
Cuts at: 0 493 1869
Size: 493 1376
SapI GCTCTTCn'nnn_
Cuts at: 0 1618 1869
Size: 1618 251
ScaI AGT'ACT
Cuts at: 0 249 1869
Size: 249 1620
SfcI C'TryA_G
Cuts at: 0 1087 1869
Size: 1087 782
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1063 1588 1869
Size: 1063 525 281
Fragments arranged by size:
1063 525 281
SmlI C'TyrA_G
Cuts at: 0 209 443 1813 1869
Size: 209 234 1370 56
Fragments arranged by size:
1370 234 209 56
StyI C'CwwG_G
Cuts at: 0 170 1869
Size: 170 1699
TatI w'GTAC_w
Cuts at: 0 247 1442 1869
Size: 247 1195 427
Fragments arranged by size:
1195 427 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 1801 1833 1869
Size: 538 32 1231 32 36
Fragments arranged by size:
1231 538 36 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1140 1869
Size: 1140 729
XbaI T'CTAG_A
Cuts at: 0 1245 1869
Size: 1245 624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1635 1659 1869
Size: 1635 24 210
Fragments arranged by size:
1635 210 24
XhoI C'TCGA_G
Cuts at: 0 209 443 1869
Size: 209 234 1426
Fragments arranged by size:
1426 234 209
Enzymes that do cut:
AarI AccI AcuI AleI ApaI ApoI AvaI BanII
BbsI BclI BglI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BssSI
BstXI BstYI BstZ17I BtsI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NspI PflMI PfoI PpuMI
PspOMI PspXI PvuII SacI SalI SapI ScaI SfcI
SfiI SmlI StyI TatI TstI Tth111I XbaI XcmI
XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BlpI BmgBI BmtI BplI BsaAI BsaBI BsgI BsiEI
BsiWI BsmI BsrFI BsrGI BssHII BstAPI BstBI BstEII
Bsu36I BtgI BtgZI ClaI CspCI DrdI EagI EciI
EcoNI EcoRI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PsrI PstI PvuI RsrII
SacII SanDI SbfI SexAI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
XmaI XmnI ZraI