Restriction map for
pFN21AE6417
(Linear) (Six-base) MAPSORT of: KIEE6417.seq Check: 3431 from: 1 to: 1680
KIEE6417.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1585 1680
Size: 1585 95
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1680
Size: 202 1478
AflIII A'CryG_T
Cuts at: 0 35 434 1680
Size: 35 399 1246
Fragments arranged by size:
1246 399 35
AleI CACnn'nnGTG
Cuts at: 0 898 1306 1680
Size: 898 408 374
Fragments arranged by size:
898 408 374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 679 1680
Size: 679 1001
AlwNI CAG_nnn'CTG
Cuts at: 0 746 1002 1237 1680
Size: 746 256 235 443
Fragments arranged by size:
746 443 256 235
ApaI G_GGCC'C
Cuts at: 0 732 1573 1680
Size: 732 841 107
Fragments arranged by size:
841 732 107
AvaI C'yCGr_G
Cuts at: 0 616 1445 1680
Size: 616 829 235
Fragments arranged by size:
829 616 235
BamHI G'GATC_C
Cuts at: 0 542 1680
Size: 542 1138
BanI G'GyrC_C
Cuts at: 0 3 119 516 643 1117 1456 1680
Size: 3 116 397 127 474 339 224
Fragments arranged by size:
474 397 339 224 127 116 3
BanII G_rGCy'C
Cuts at: 0 167 403 732 926 1359 1562 1573 1680
Size: 167 236 329 194 433 203 11 107
Fragments arranged by size:
433 329 236 203 194 167 107 11
BbeI G_GCGC'C
Cuts at: 0 123 1680
Size: 123 1557
BbsI GAAGACnn'nnnn_
Cuts at: 0 669 969 1680
Size: 669 300 711
Fragments arranged by size:
711 669 300
BglI GCCn_nnn'nGGC
Cuts at: 0 19 98 263 1680
Size: 19 79 165 1417
Fragments arranged by size:
1417 165 79 19
BlpI GC'TnA_GC
Cuts at: 0 402 1032 1680
Size: 402 630 648
Fragments arranged by size:
648 630 402
Bme1580I G_kGCm'C
Cuts at: 0 646 732 1573 1680
Size: 646 86 841 107
Fragments arranged by size:
841 646 107 86
BmgBI CAC'GTC
Cuts at: 0 36 1590 1680
Size: 36 1554 90
Fragments arranged by size:
1554 90 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 487 1362 1680
Size: 487 875 318
Fragments arranged by size:
875 487 318
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 591 1680
Size: 591 1089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 195 862 1011 1087 1483 1680
Size: 179 16 667 149 76 396 197
Fragments arranged by size:
667 396 197 179 149 76 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 1680
Size: 129 1551
BsaI GGTCTCn'nnnn_
Cuts at: 0 213 336 361 1474 1663 1680
Size: 213 123 25 1113 189 17
Fragments arranged by size:
1113 213 189 123 25 17
BsaBI GATnn'nnATC
Cuts at: 0 190 547 1680
Size: 190 357 1133
Fragments arranged by size:
1133 357 190
BsaHI Gr'CG_yC
Cuts at: 0 120 266 1680
Size: 120 146 1414
Fragments arranged by size:
1414 146 120
BsaWI w'CCGG_w
Cuts at: 0 223 428 1680
Size: 223 205 1252
Fragments arranged by size:
1252 223 205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 85 524 538 580 1014 1630 1680
Size: 46 39 439 14 42 434 616 50
Fragments arranged by size:
616 439 434 50 46 42 39 14
BseYI C'CCAG_C
Cuts at: 0 441 666 705 876 1389 1517 1680
Size: 441 225 39 171 513 128 163
Fragments arranged by size:
513 441 225 171 163 128 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 604 1629 1680
Size: 60 544 1025 51
Fragments arranged by size:
1025 544 60 51
BsiEI CG_ry'CG
Cuts at: 0 1086 1680
Size: 1086 594
BsiHKAI G_wGCw'C
Cuts at: 0 167 403 917 1301 1562 1680
Size: 167 236 514 384 261 118
Fragments arranged by size:
514 384 261 236 167 118
Bsp1286I G_dGCh'C
Cuts at: 0 167 403 646 732 917 926 1301 1359
Size: 167 236 243 86 185 9 375 58
Cuts at: 1359 1562 1573 1680
Size: 203 11 107
Fragments arranged by size:
375 243 236 203 185 167 107 86
58 11 9
BspEI T'CCGG_A
Cuts at: 0 223 428 1680
Size: 223 205 1252
Fragments arranged by size:
1252 223 205
BspHI T'CATG_A
Cuts at: 0 395 770 1680
Size: 395 375 910
Fragments arranged by size:
910 395 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1585 1680
Size: 1585 95
BsrBI CCG'CTC
Cuts at: 0 1608 1680
Size: 1608 72
BsrDI GCAATG_nn'
Cuts at: 0 824 1340 1680
Size: 824 516 340
Fragments arranged by size:
824 516 340
BsrFI r'CCGG_y
Cuts at: 0 1085 1273 1680
Size: 1085 188 407
Fragments arranged by size:
1085 407 188
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1002 1680
Size: 1002 678
BstXI CCAn_nnnn'nTGG
Cuts at: 0 157 303 1131 1525 1680
Size: 157 146 828 394 155
Fragments arranged by size:
828 394 157 155 146
BstYI r'GATC_y
Cuts at: 0 542 836 1680
Size: 542 294 844
Fragments arranged by size:
844 542 294
BtgI C'CryG_G
Cuts at: 0 275 371 692 896 918 984 1112 1286
Size: 275 96 321 204 22 66 128 174
Cuts at: 1286 1307 1680
Size: 21 373
Fragments arranged by size:
373 321 275 204 174 128 96 66
22 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 665 993 1077 1158 1680
Size: 665 328 84 81 522
Fragments arranged by size:
665 522 328 84 81
BtsI GCAGTG_nn'
Cuts at: 0 1039 1139 1235 1641 1680
Size: 1039 100 96 406 39
Fragments arranged by size:
1039 406 100 96 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 477 512 1680
Size: 477 35 1168
Fragments arranged by size:
1168 477 35
EaeI y'GGCC_r
Cuts at: 0 1016 1160 1206 1680
Size: 1016 144 46 474
Fragments arranged by size:
1016 474 144 46
EarI CTCTTCn'nnn_
Cuts at: 0 1531 1680
Size: 1531 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 60 555 1680
Size: 60 495 1125
Fragments arranged by size:
1125 495 60
EcoICRI GAG'CTC
Cuts at: 0 165 401 1560 1680
Size: 165 236 1159 120
Fragments arranged by size:
1159 236 165 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 195 202 862 1011 1087 1483 1680
Size: 179 16 7 660 149 76 396 197
Fragments arranged by size:
660 396 197 179 149 76 16 7
EcoO109I rG'GnC_Cy
Cuts at: 0 113 528 728 729 1570 1680
Size: 113 415 200 1 841 110
Fragments arranged by size:
841 415 200 113 110 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 897 1680
Size: 897 783
FseI GG_CCGG'CC
Cuts at: 0 1277 1680
Size: 1277 403
HaeII r_GCGC'y
Cuts at: 0 123 1680
Size: 123 1557
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 1680
Size: 44 32 1604
Fragments arranged by size:
1604 44 32
HincII GTy'rAC
Cuts at: 0 1185 1350 1680
Size: 1185 165 330
Fragments arranged by size:
1185 330 165
KasI G'GCGC_C
Cuts at: 0 119 1680
Size: 119 1561
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 1411 1680
Size: 398 1013 269
Fragments arranged by size:
1013 398 269
MscI TGG'CCA
Cuts at: 0 1018 1208 1680
Size: 1018 190 472
Fragments arranged by size:
1018 472 190
MslI CAynn'nnrTG
Cuts at: 0 301 325 661 898 1129 1306 1680
Size: 301 24 336 237 231 177 374
Fragments arranged by size:
374 336 301 237 231 177 24
MspA1I CmG'CkG
Cuts at: 0 725 986 1258 1408 1627 1680
Size: 725 261 272 150 219 53
Fragments arranged by size:
725 272 261 219 150 53
NaeI GCC'GGC
Cuts at: 0 1275 1680
Size: 1275 405
NarI GG'CG_CC
Cuts at: 0 120 1680
Size: 120 1560
NcoI C'CATG_G
Cuts at: 0 692 1286 1680
Size: 692 594 394
Fragments arranged by size:
692 594 394
NgoMIV G'CCGG_C
Cuts at: 0 1273 1680
Size: 1273 407
NspI r_CATG'y
Cuts at: 0 13 438 1680
Size: 13 425 1242
Fragments arranged by size:
1242 425 13
PasI CC'CwG_GG
Cuts at: 0 857 1566 1680
Size: 857 709 114
Fragments arranged by size:
857 709 114
PciI A'CATG_T
Cuts at: 0 434 1680
Size: 434 1246
PfoI T'CCnGG_A
Cuts at: 0 560 839 1499 1680
Size: 560 279 660 181
Fragments arranged by size:
660 560 279 181
PpuMI rG'GwC_Cy
Cuts at: 0 113 1680
Size: 113 1567
PshAI GACnn'nnGTC
Cuts at: 0 1675 1680
Size: 1675 5
PspOMI G'GGCC_C
Cuts at: 0 728 1569 1680
Size: 728 841 111
Fragments arranged by size:
841 728 111
PstI C_TGCA'G
Cuts at: 0 998 1242 1680
Size: 998 244 438
Fragments arranged by size:
998 438 244
PvuII CAG'CTG
Cuts at: 0 1258 1680
Size: 1258 422
SacI G_AGCT'C
Cuts at: 0 167 403 1562 1680
Size: 167 236 1159 118
Fragments arranged by size:
1159 236 167 118
SacII CC_GC'GG
Cuts at: 0 987 1680
Size: 987 693
SbfI CC_TGCA'GG
Cuts at: 0 998 1680
Size: 998 682
SfcI C'TryA_G
Cuts at: 0 994 1238 1680
Size: 994 244 442
Fragments arranged by size:
994 442 244
SfoI GGC'GCC
Cuts at: 0 121 1680
Size: 121 1559
SmaI CCC'GGG
Cuts at: 0 1447 1680
Size: 1447 233
SmlI C'TyrA_G
Cuts at: 0 108 1680
Size: 108 1572
SphI G_CATG'C
Cuts at: 0 13 1680
Size: 13 1667
StuI AGG'CCT
Cuts at: 0 1552 1680
Size: 1552 128
StyI C'CwwG_G
Cuts at: 0 509 692 1286 1680
Size: 509 183 594 394
Fragments arranged by size:
594 509 394 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 359 706 756 1105 1381 1680
Size: 359 347 50 349 276 299
Fragments arranged by size:
359 349 347 299 276 50
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 568 1128 1680
Size: 568 560 552
Fragments arranged by size:
568 560 552
XmaI C'CCGG_G
Cuts at: 0 1445 1680
Size: 1445 235
XmnI GAAnn'nnTTC
Cuts at: 0 436 1680
Size: 436 1244
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI
BtgI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI FseI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SbfI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BciVI BclI BfrBI BglII BmtI Bpu10I
BsaAI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I ClaI DraI DraIII DrdI
EagI EcoNI EcoRI EcoRV FspI FspAI HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SapI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI ZraI